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STOCK Ntn5tm1.1Rwb/Rwb
Stock No: 005592
  • Targeted Mutation
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  • Overview
  • Details
    • Detailed Description
    • Development
    • Control Suggestions
    • Selected References
  • Genetics
  • Disease/Phenotype
    • Disease Terms
    • Research Areas By Phenotype
    • Mammalian Phenotype Terms by Genotype
    • References
  • Technical Support
    • Genotyping Protocols
    • Breeding Considerations
    • Mating System
    • Breeder Pairs
    • Citation
    • Animal Health Reports
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  • Pricing & Availability
    • Price
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  • Terms Of Use
  • Related Strains

Overview

Mice homozygous for this deletion have ectopic motor neurons that migrate out of the ventral horn and into the motor roots, with more ectopias in the rostral than the caudal end. This strain is valuable in the characterization of netrin family signaling and neuronal development.

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Genetic overview

Genetic Background Generation

Ntn5tm1.1Rwb

Allele Type Gene Symbol Gene Name
Targeted (Null/Knockout) Ntn5 netrin 5
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Research Applications

  • Neurobiology Research
  • Developmental Biology Research
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Base Price

Starting at:

$50.00 Domestic price for female
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Details

Detailed Description

Mice homozygous for this deletion have ectopic motor neurons that migrate out of the ventral horn and into the motor roots, with more ectopias in the rostral than the caudal end. These ectopic neurons do not express neuropilin 2. Despite the ectopic motor neurons, homozygotes are overtly normal, with normal body size, life span, fertility, gait, grip strength, and general motor activity, and no aberrant phenotypes detected in the Knockout Mouse Phenotyping (KOMP2) assessment performed at The Jackson Laboratory. Although Ntn5 is expressed in boundary cap cells, it is not required for their development as boundary cells are present in homozygotes. The trigeminal nerve is normal, dorsal root ganglia and their sensory inputs into the dorsal spinal cord are normal, and no nociceptive defects were detected by either von Frey or hot plate testing.

Development

Using homologous recombination in R1 ES cells, exons 2-6 were replaced with farnysylated YFP fused to the 5-prime sequence of exon2 and an FRT-flanked neomycin resistance cassette and the resulting mice were bred to B6.Cg-Tg(ACTFLPe)9205Dym/J to excise the neomycin resistance cassette. Although sequencing confirmed that the integrated YFPF is in-frame with the start site, no expression was detected by either endogenous fluorescence or antibody staining. This strain was crossed to C57BL/6J for approximately 3 generations before being maintained by sibling intercrossing primarily homozygotes.

Control Suggestions

Approximate Controls

  • 000664 C57BL/6J

Additional Information

  • Considerations for Choosing Controls

Selected References

  • Garrett AM; Jucius TJ; Sigaud LP; Tang FL; Xiong WC; Ackerman SL; Burgess RW. 2016. Analysis of Expression Pattern and Genetic Deletion of Netrin5 in the Developing Mouse. Front Mol Neurosci 9:3PubMed: 26858598MGI: J:227617
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Genetics

Ntn5tm1.1Rwb

Allele Symbol: Ntn5tm1.1Rwb

Allele Name targeted mutation 1.1, Robert W Burgess
Allele Type Targeted (Null/Knockout)
Allele Synonym(s)
Gene Symbol and Name Ntn5, netrin 5
Gene Synonym(s)
Strain of Origin (129X1/SvJ x 129S1/Sv)F1-Kitl+
Chromosome 7
Molecular Note Exons 2-6 were replaced with a cassette encoding a farnysylated YFP fused to the 5-prime sequence of exon2 and an FRT-flanked neomycin resistance cassette, and subsequent flpe-mediated excision deleted the neomycin selection cassette. Although sequencing confirmed that the integrated YFPF is in-frame with the start site, no expression was detected by either endogenous fluorescence or antibody staining. Although this deletion is also in intron 1 of Sec1, Q-RT-PCR did not detect any reduction in Sec1 expression.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Ntn5tm1.1Rwb related

  • Neurobiology Research
  • Developmental Biology Research
    • Neurodevelopmental Defects

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: Ntn5tm1.1Rwb/Ntn5tm1.1Rwb
STOCK Ntn5/Rwb

mammalian phenotype

  • behavior/neurological phenotype
    • Normal - grip strength, gait, and general motor activity, including characterization in the Knockout Mouse Phenotyping (KOMP2) project, are all normal
    • (MGI Ref ID J:227617)
  • nervous system phenotype
    • Normal - boundary cap cells are present, and there are no abnormalities in cranial nerve guidance, dorsal root organization, sensory projections, and no nociceptive defects were detected by either von Frey or hot plate testing
    • (MGI Ref ID J:227617)

nervous system phenotype

  • ectopic neuron
    • homozygotes have ectopic motor neurons that migrate out of the ventral horn and into the motor roots, with more ectopias in the rostral than the caudal end, and these ectopic neurons do not express neuropilin 2
    • (MGI Ref ID J:227617)

References

  • Garrett AM; Jucius TJ; Sigaud LP; Tang FL; Xiong WC; Ackerman SL; Burgess RW. 2016. Analysis of Expression Pattern and Genetic Deletion of Netrin5 in the Developing Mouse. Front Mol Neurosci 9:3PubMed: 26858598MGI: J:227617

Additional - Ntn5tm1.1Rwb related

Technical Support

CONTACT TECHNICAL SUPPORT
  • Genotyping Protocols

    • Genotyping resources and troubleshooting
  • Breeding Considerations

    homozygotes are viable and fertile

    • Additional Breeding and Husbandry Support
  • Mating System

    • Heterozygote x Heterozygote
  • Citation

    When using the STOCK Ntn5tm1.1Rwb/Rwb mouse strain in a publication, please cite the originating article(s) and include JAX stock #005592 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

A6 (Intermediate)

Pricing & Availability

Availability Varies
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Live Mouse

Age Genotype Price
weeks

Cryorecovery - Pricing

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Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

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Licensing Information

Phone: 207-288-6470
Email: TechTran@jax.org

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