Optogenetics Strains

JAX# COMMON NAME PROMOTER EFFECTOR EXPRESSION STRAIN NOMENCLATURE (Nov 2017)
             
Chemogenetic Lines STRAIN NOMENCLATURE
014093 TRE-hM3Dq TRE activation Gq-DREADD (hM3Dq) Tet- & CNO-inducible Gq pathway (burst firing) STOCK Tg(tetO-CHRM3*)1Blr/J
026220 R26-LSL-Gq-DREADD R26 :: CAG activ. Gq-DREADD (hM3Dq) :: mCitrine Cre-inducible mCitrine ; then CNO-inducible Gq pathway  B6N;129-Tg(CAG-CHRM3*,-mCitrine)1Ute/J
026942 RC::FL-hM3Dq R26 :: CAG activ. Gq-DREADD (hM3Dq) / mCherry FLP-induc. eGFP ; then Cre-induc. mCherry ; then CNO-induc. Gq pathway B6.Cg-Gt(ROSA)26Sortm3.2(CAG-EGFP,-CHRM3*/mCherry/Htr2a)Pjen/J
026943 RC::L-hM3Dq R26 :: CAG activ. Gq-DREADD (hM3Dq) / mCherry Cre-inducible mCherry ; then CNO-inducible Gq pathway B6.Cg-Gt(ROSA)26Sortm3.3(CAG-EGFP,-CHRM3*/mCherry/Htr2a)Pjen/J
024114 TRE-hM4Di TRE inhibitory Gi-DREADD (hM4Di) Tet- & CNO-inducible Gi pathway (neuron silencing) B6.Cg-Tg(tetO-CHRM4*)2Blr/J
026219 R26-LSL-Gi-DREADD R26 :: CAG inhib. Gi-DREADD (hM4Di) :: mCitrine Cre-inducible mCitrine ; then CNO-inducible Gi pathway B6N.129-Gt(ROSA)26Sortm1(CAG-CHRM4*,-mCitrine)Ute/J
029040 RC::FPDi R26 :: CAG inhib. Gi-DREADD (hM4Di) :: mCherry FLP-induc. mCherry, then Cre- & CNO-induc. Gi pathway B6;129S6-Gt(ROSA)26Sortm9(CAG-mCherry,-CHRM4*)Dym/J
029301 TRE-GlyCl line 9531 TRE glycine-insensitive (IVM-inducible) chloride channel :: eYFP Tet- & IVM-inducible hyperpolarizing Cl current FVB.Cg-Tg(tetO/Prnp-GLRA1*,-EYFP)9531Jjan/J
             
Thy1-GCaMP6 Lines from GENIE Project (Janelia/HHMI) STRAIN NOMENCLATURE
024275 GP4.3 thymus cell antigen 1 GCaMP6s high (hippocampus); mod (M1 S1 V1) C57BL/6J-Tg(Thy1-GCaMP6s)GP4.3Dkim/J
025776 GP4.12 thymus cell antigen 1 GCaMP6s high in M1, amygdala, S1, hippocampus C57BL/6J-Tg(Thy1-GCaMP6s)GP4.12Dkim/J
028279 GP5.1 thymus cell antigen 1 GCaMP6f high (hippocampus); mod (M1 S1 V1 pirif. ob) C57BL/6J-Tg(Thy1-GCaMP6f)GP5.1Dkim/J
024276 GP5.5 thymus cell antigen 1 GCaMP6f high (hippocampus); mod (M1 S1 V1 amyg ob) C57BL/6J-Tg(Thy1-GCaMP6f)GP5.5Dkim/J
024339 GP5.11 thymus cell antigen 1 GCaMP6f moderate in hippocampus, M1, S1, V1, olf. bulb C57BL/6J-Tg(Thy1-GCaMP6f)GP5.11Dkim/J
025393 GP5.17 thymus cell antigen 1 GCaMP6f high in hippocampus, M1, S1, V1, amygdala, olf. bulb C57BL/6J-Tg(Thy1-GCaMP6f)GP5.17Dkim/J
             
Voltage Indicators : OptoPatch and CaViAr STRAIN NOMENCLATURE
028678 Floxopatch, Optopatch2 R26 :: CAG CheRiff-eGFP :: QuasAr2-mOrange2 Cre-dependent ; all-optical electrophysiology B6;129S6-Gt(ROSA)26Sortm1Acoh/J
029677 Optopatch2 Ai141 TIGRE :: TRE CheRiff-eGFP :: QuasAr2-mOrange2 Cre- & Tet-dependent ; all-optical electrophysiology B6;129S6-Igs7tm2(tetO-Optopatch2)Acoh/J
029679 Optopatch3 Ai155 TIGRE :: TRE CheRiff-eGFP :: QuasAr3-mCitrine Cre- & Tet-dependent ; all-optical electrophysiology B6;129S6-Igs7tm3(tetO-Optopatch3,CAG-tTA)Acoh/J
029678 CaViAr TIGRE :: TRE QuasAr2-mOrange2 ::
GCaMP6f [membrane]
Cre- & Tet-dependent ; Ca2+ & voltage imaging B6;129S6-Igs7tm1(tetO-QuasAr2/GCaMP6f)Acoh/J
             
Specific Promoter Lines STRAIN NOMENCLATURE
022486 UBC PA-GFP human ubiquitin C PA-GFP widespread B6.Cg-Ptprca Tg(UBC-PA-GFP)1Mnz/J
007612 Thy1-ChR2-EYFP line 18 thymus cell antigen 1 ChR2 / EYFP widespread brain B6.Cg-Tg(Thy1-COP4/EYFP)18Gfng/J
007615 Thy1-ChR2-EYFP line 9 thymus cell antigen 1 ChR2 / EYFP widespread brain B6.Cg-Tg(Thy1-COP4/EYFP)9Gfng/J
012355 PV-hChR2(H134R)-EYFP line 15 parvalbumin mhChR2 / EYFP PV+ GABAergic interneurons B6;SJL-Tg(Pvalb-COP4*H134R/EYFP)15Gfng/J
014546 ChAT-ChR2(H134R)-EYFP line 6 choline acetyltransferase  mhChR2 / EYFP cholinergic neurons B6.Cg-Tg(Chat-COP4*H134R/EYFP,Slc18a3)6Gfng/J
014548 VGAT-ChR2(H134R)-EYFP vesicular GABA transporter mhChR2 / EYFP GABAergic interneurons B6.Cg-Tg(Slc32a1-COP4*H134R/EYFP)8Gfng/J
021206 M72-IRES-ChR2-YFP olfactory receptor 160 ChR2*H134R / EYFP M72+ olfactory sensory neurons STOCK Olfr160tm1.1(COP4*/EYFP)Tboz/J
029860 Thy1-GCaMP3 line 6 (C57BL/6) thymus cell antigen 1 GCaMP3 increased GFP in activated neurons B6.Cg-Tg(Thy1-GCaMP3)6Gfng/J
024742 TRE-GCaMP6s line G6s2 TRE GCaMP6s Tet-inducible B6;DBA-Tg(tetO-GCaMP6s)2Niell/J
025111 Snap25-2A-GCaMP6s-D synaptosomal-assoc. prot.25 GCaMP6s widespread brain B6.Cg-Snap25tm3.1Hze/J
030613 hArl13b-mCherry-GECO1.2tg CAG GECO1.2 / mCherry primary cilia C57BL/6J-Tg(CAG-ARL13B/mCherry/GECO1.2)1Clph/J
030525 Thy1-jRGECO1a-WPRE, line GP8.20 thymus cell antigen 1 jRGECO1a brain (denser cortex) C57BL/6J-Tg(Thy1-jRGECO1a)GP8.20Dkim/J
030526 Thy1-jRGECO1a-WPRE, line GP8.31 thymus cell antigen 1 jRGECO1a brain (sparser cortex) C57BL/6J-Tg(Thy1-jRGECO1a)GP8.31Dkim/J
030527 Thy1-jRGECO1a-WPRE, line GP8.58 thymus cell antigen 1 jRGECO1a brain C57BL/6J-Tg(Thy1-jRGECO1a)GP8.58Dkim/J
030528 Thy1-jRGECO1a-WPRE, line GP8.62 thymus cell antigen 1 jRGECO1a brain C57BL/6J-Tg(Thy1-jRGECO1a)GP8.62Dkim/J
             
Cre-Inducible Lines STRAIN NOMENCLATURE
012735 Ai35D R26 :: CAG Arch / GFP Cre-dependent B6;129S-Gt(ROSA)26Sortm35.1(CAG-aop3/GFP)Hze/J
021188 Ai40D R26 :: CAG ArchT / EGFP Cre-dependent B6.Cg-Gt(ROSA)26Sortm40.1(CAG-aop3/EGFP)Hze/J
017455 2xChETA-tdT R26 :: CAG ChETA/ChR2*H134R and tdT Cre-dependent B6;129-Gt(ROSA)26Sortm1(CAG-COP4*E123T*H134R,-tdTomato)Gfng/J
024109 Ai32 R26 :: CAG ChR2*H134R / EYFP Cre-dependent B6.Cg-Gt(ROSA)26Sortm32(CAG-COP4*H134R/EYFP)Hze/J
012567 Ai27D R26 :: CAG hChR2*H134R / tdTomato Cre-dependent B6.Cg-Gt(ROSA)26Sortm1(CAG-COP4*E123T*H134R,-tdTomato)Gfng/J
026294 Rosa26 CAG-LSL-ReaChR-mCit R26 :: CAG ReaChR / mCitrine Cre-dependent B6.Cg-Gt(ROSA)26Sortm2.2Ksvo/J
014539 Ai39 R26 :: CAG eNpHR3.0 / EYFP Cre-dependent B6;129S-Gt(ROSA)26Sortm39(CAG-hop/EYFP)Hze/J
014538 Ai38 R26 :: CAG GCaMP3 Cre-dependent B6;129S-Gt(ROSA)26Sortm38(CAG-GCaMP3)Hze/J
024477 PC::G5-tdT RNA pol II :: CAG GCaMP5G Cre-dependent B6;129S6-Polr2atm1(CAG-GCaMP5g,-tdTomato)Tvrd/J
029626 R26-Lck-GCaMP6f flox R26 :: CAG GCaMP6f [membrane] Cre-dependent C57BL/6N-Gt(ROSA)26Sortm1(CAG-GCaMP6f)Khakh/J
028865 Ai95D (C57BL/6J) R26 :: CAG GCaMP6f Cre-dependent B6J.Cg-Gt(ROSA)26Sortm95.1(CAG-GCaMP6f)Hze/MwarJ
028866 Ai96 (C57BL/6J) R26 :: CAG GCaMP6s Cre-dependent B6J.Cg-Gt(ROSA)26Sortm96(CAG-GCaMP6s)Hze/MwarJ
028583 RGBow reporter R26 :: CAG mOrange2 ; EGFP ; mKate2 Cre-dependent B6;129S4-Gt(ROSA)26Sortm4(CAG-mOrange2,-EGFP,-mKate2)Zjh/J
012570 Ai34D R26 :: CAG Synaptophysin / tdTomato Cre-dependent B6;129S-Gt(ROSA)26Sortm34.1(CAG-Syp/tdTomato)Hze/J
031102 Ai35-hVOS1.5 R26 :: CAG hVOS1.5 hybrid voltage sensor  Cre-dependent C57BL/6-Gt(ROSA)26Sortm1(CAG-hVOS1.5)Mbja/J
         
Cre:Tet-Inducible Lines from Allen Institute STRAIN NOMENCLATURE
024103 Ai93D TIGRE :: TRE GCaMP6f Cre- and Tet-dependent B6;129S6-Igs7tm93.1(tetO-GCaMP6f)Hze/J
024107 Ai93D;ROSA26-ZtTA TIGRE :: TRE ; R26 GCaMP6f + tTA widespread before dox STOCK Gt(ROSA)26Sortm5(ACTB-tTA)Luo Igs7tm93.1(tetO-GCaMP6f)Hze/HzeJ
024108 Ai93D;CaMK2a-tTA TIGRE :: TRE ; CaMK2a GCaMP6f + tTA forebrain before dox STOCK Igs7tm93.1(tetO-GCaMP6f)Hze Tg(Camk2a-tTA)1Mmay/J
030328 Ai148D TIGRE :: TRE2 + CAG GCaMP6f + tTA2 Cre- and Tet-dependent B6.Cg-Igs7tm148.1(tetO-GCaMP6f,CAG-tTA2)Hze/J
024104 Ai94D TIGRE :: TRE GCaMP6s Cre- and Tet-dependent B6.Cg-Igs7tm94.1(tetO-GCaMP6s)Hze/J
024112 Ai94D;ROSA26-ZtTA TIGRE :: TRE ; R26 GCaMP6s + tTA widespread before dox STOCK Gt(ROSA)26Sortm5(ACTB-tTA)Luo Igs7tm94.1(tetO-GCaMP6s)Hze/J
024115 Ai94D;CaMK2a-tTA TIGRE :: TRE ; CaMK2a GCaMP6s + tTA forebrain before dox B6.Cg-Igs7tm94.1(tetO-GCaMP6s)Hze Tg(Camk2a-tTA)1Mmay/J
030217 Ai143D TIGRE :: TRE RCaMP1.07 Cre- and Tet-dependent B6.Cg-Igs7tm143.1(tetO-RCaMP1.07)Hze/J
026260 Ai85D TIGRE :: TRE iGluSnFr Cre- and Tet-dependent B6;129S-Igs7tm85.1(tetO-gltI/GFP*)Hze/J
023529 Ai79D TIGRE :: TRE Jaws (Halo57*K200R*W214F) / EGFP Cre- and Tet-dependent B6;129S-Igs7tm79.1(tetO-hop/EGFP)Hze/J
030216 Ai136D TIGRE :: TRE ReaChR / EYFP Cre- and Tet-dependent B6.Cg-Igs7tm136.1(tetO-COP3*/COP4*/EYFP)Hze/J
024100 Ai90D TIGRE :: TRE ShChR (Chronos) Cre- and Tet-dependent B6.Cg-Igs7tm90.1(tetO-COP4*/EGFP)Hze/J
030213 Ai133D TIGRE :: TRE ssAPEX2tm Cre- and Tet-dependent B6.Cg-Igs7tm133.1(tetO-apx1*)Hze/J
030219 Ai139D TIGRE :: TRE2 + CAG EGFP + tdT + tTA2 Cre- and Tet-dependent B6.Cg-Igs7tm139.1(tetO-EGFP,CAG-tdTomato,-tTA2)Hze/J
030220 Ai140D TIGRE :: TRE2 + CAG EGFP + tTA2 Cre- and Tet-dependent B6.Cg-Igs7tm140.1(tetO-EGFP,CAG-tTA2)Hze/J
             
Cre:FLP Dual Inducible Lines STRAIN NOMENCLATURE
025109 Ai80D R26 :: CAG CatCh (ChR2*L132C) / EYFP Cre- and FLP-dependent B6.Cg-Gt(ROSA)26Sortm80.1(CAG-COP4*L132C/EYFP)Hze/J
024846 Rosa26 CAG-FSF-LSL-ReaChR-mCit R26 :: CAG ReaChR / mCitrine Cre- and FLP-dependent B6;129S-Gt(ROSA)26Sortm2.1Ksvo/J
030206 RC::FPSit R26 :: CAG Synaptophysin / EGFP + tdTomato Cre- and FLP-dependent B6;129S6-Gt(ROSA)26Sortm10(CAG-Syp/EGFP*,-tdTomato)Dym/J
021875 Ai65D R26 :: CAG tdTomato Cre- and FLP-dependent B6;129S-Gt(ROSA)26Sortm65.1(CAG-tdTomato)Hze/J
026932 RC::FLTG R26 :: CAG tdTomato ; EGFP FLP-dependent tdT ; then Cre-dependent EGFP B6.Cg-Gt(ROSA)26Sortm1.3(CAG-tdTomato,-EGFP)Pjen/J