Data organization protocol

Protocol for: Protocol for data organization
Prepared by: Rong Yuan
Reviewed by: David Harrison
Last revision date: 05/01/2006

Protocol for data organization

1. – Data constitutes:

  • All the hardcopies and electronic copies of the experiment records, which include all the relevant mouse information such as strain name, sex, DOB, chip # for the mouse
  • Also the results obtained from performing the experiments
  • And plasma stock information

2. – Methods for backing up data:

2.a – Hard copies of all the experiment records and templates are organized into folders.

2.b – All data is in the shared folder.

2.c – A back-up CD is burnt every Friday.

2.d – A new file is created each time the master inventory file is updated. All the older versions of the inventory file are saved. Hence, there is always the possibility of going back and being able to trace a particular modification.

In due course, all the inventory and experiment design information will be transferred into the JaxTrack.

3. – Every mouse undergoes the following tests:

Immuno-aging Markers, Hematology assays, Chemistry Analysis, Hormone tests (IGF-1, Glucose, Insulin, Leptin), CCMS, Grip, Gait and Urine assays.

4. – Linking samples, phenotype data and an individual mouse:

4.a – Every mouse tested has a chip (the chip number is unique). Chip number can be easily read and saved into our inventory file or online database.

4.b – Every sample that is obtained from one of the physiological tests has a unique sample ID. Each tube containing a plasma/blood sample is tagged with a printed label that is pasted to the tube.

4.c – The chip number along with other information such as strain name, sex, DOB, test day, protocol used are saved in the hard and the soft copies of the experiment record. The sample ID information is linked to all of the above.

4.d – Most of our plasma samples are saved in a freezer box at -70. The box ID for each sample is recorded.

4.f – Any of the samples that are sent for testing are recorded and from the sample ID the mouse information including Chip ID can be matched. From the Chip ID all other test parameters for the mouse can be tracked.