Blood plasma chemistry protocol

0.0 Abstract: The Beckman Coulter Synchron CX5 (“Chemistry Analyzer”) is used to measure four blood plasma constituents for this PGA: HDL cholesterol (HDL), total cholesterol (CHOL), triglycerides (TG), and glucose (GLU).

1.0 Instrument layout: A dedicated DOS-based desktop computer controls the programming of the Chemistry Analyzer. A dedicated printer prints the results as they are measured, and an electronic file is simultaneously transferred to a second, Windows-based computer, which stores the data files.

2.0 Reagents and expendables

2.1 Expendables: The following supplies and quantities are used for each sample tested (for all four of the above chemistries): 1.5 m l Eppendorf tube (1), 0.5ml Beckman Coulter Microtube™ Tubecup (“sector cup”) (2), and 200 m l pipette tips (3-4, depending on whether dilution is necessary).

2.2 Reagents: The Chemistry Analyzer (or “CX5”) uses Beckman Coulter three-compartment reagent cartridges for HDLc, CHOL, TG, and GLU. Each cartridge contains enough reagent for 300 tests (approx. 104mL). In addition, in order to run the HDL Cholesterol test, HDL Cholesterol Separation Reagent (15 m l per sample) is needed. The bottle from Beckman Coulter contains a volume of 34ml. If the dilution of plasma samples becomes necessary due to low plasma volume, use 0.9% saline solution for the dilution.

Reagent: Reorder Number:

Cholesterol (CHOL) Reagent 467825

Glucose (GLU) Reagent 442640

Triglycerides (TG) Reagent 445850

2.3 Calibration reagents: The two calibration reagents are “Synchron® Systems HDL Cholesterol Calibrator” (for HDL only), and “Synchron® Systems Multi Calibrator” (for CHOL, GLU, and TG).

2.4 Controls : The controls for HDLc are Beckman Coulter™ Vigil™ Lipid Control 1 and Beckman Coulter™ Vigil™ Lipid Control 2. The controls for CHOL, GLU and TG are Synchron® Control Comprehensive Chemistry Control Serum Level 1, Level 2 and Level 3.

3.0 Setup:

3.1 Reagent preparation: Us