For in-depth product & services help, ask our
Technical Information Scientists
>chrX:102504962-102505067 106bp GAGATGGAGATGCCAGAGGA GGACCTTCACAAGGGAGTCG
Mutant = G, G, C
Wild type = T, T, T
X-Linked
AGATGGAGATGCCAGAGGAACCCGCCAACAGTGGGCATTCACTACCCCCGGT(t/g)TATA(tt/gc)TACAGTCCCGAGTATGTCAGCATCTGCGACTCCCTTGTGAAGGTC
Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
55258 | GAG ATG GAG ATG CCA GAG GA | Forward | A | |||
55259 | GGA CCT TCA CAA GGG AGT CG | Reverse | A | |||
55260 | Fluorophore-1 | CCG GTT TAT ATT TAC AGT CCC G | Quencher-1 | WT Probe | ||
55261 | Fluorophore-2 | CCG GTG TAT AGC TAC AGT CCC G | Quencher-2 | MUT Probe |
Component | Final Concentration |
---|---|
Kapa Probe Fast QPCR | 1.00 X |
ddH2O | |
55258 | 0.40 uM |
55259 | 0.40 uM |
Wt Probe | 0.15 uM |
Mutant Probe | 0.15 uM |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 95.0 | -- | |
2 | 95.0 | -- | |
3 | 60.0 | -- | |
4 | -- | repeat steps 2-3 for 40 cycles | |
5 | 40.0 | -- | Forever |
We use cookies to personalize our website and to analyze web traffic to improve the user experience. You may decline these cookies although certain areas of the site may not function without them. Please refer to our privacy policy for more information.