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Mutant = A, A
Wild type = G, G
>chr7:25766740-25766807 68bp CTCTTGGCACCATCACCATC TTTAGTAGCAGCCAGGCTTACC
TCTTCATCATGGCTAACATCCCACTGCCTCTTGGCACCATCACCATCCTCTGCATT(g/a)ACCT(g/a)GGTACCGACATGgtaagcctggctgctactaaaggctggagagggccacccccacacgcttg
Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
45924 | CTC TTG GCA CCA TCA CCA TC | Forward | A | |||
45925 | TTT AGT AGC AGC CAG GCT TAC C | Reverse | A | |||
45926 | Fluorophore-1 | CTG CAT TGA CCT GGG TAC C | Quencher-1 | WT Probe | ||
45927 | Fluorophore-2 | TCT GCA TTA ACC TAG GTA CCG AC | Quencher-2 | MUT Probe |
Component | Final Concentration |
---|---|
Kapa Probe Fast QPCR | 1.00 X |
ddH2O | |
45924 | 0.40 uM |
45925 | 0.40 uM |
Wt Probe | 0.15 uM |
Mutant Probe | 0.15 uM |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 95.0 | -- | |
2 | 95.0 | -- | |
3 | 60.0 | -- | |
4 | -- | repeat steps 2-3 for 40 cycles | |
5 | 40.0 | -- | Forever |