Taqman qPCR protocols are run on a real time PCR instrument. Use an appropriate instrument specific Fluorophore/Quencher combination.
chr16:23108407+23108548 142bp GTCCAAGTCCCAAGCTGTTG AGCCACGCAGAGACCCTTA
Mutant= 137 bp
Wild Type = 142 bp
Fam = Mut
Hex = Wt
Wt Sequence (deletions in lower case):
TCGGCGCGCGGAGCACTCGCGAGAGCTTCGCTTCCCAAGCGGGAGGGCCTGGTCCTAGGGCACTGAGTAAAGGGGCGTCTATACTTAGGGGTCCAAGTCCCAAGCTGTTGGGGAGGGCGAATGGCGCTTCGCAGCATTGCGGCCGGTTTTGAGGCGTTTTCAGCAGAGCacaggggcgcggaggggaatgaggtacggggtggaagggccctttaagggtctctgcgtggcttggaagcagtatgtctaactgcagtgttgttgtcagcagagaacatggcggcccagagggaatggaccccgatggtgtcatcgaggtaaggagtgggtgtggacgttaaaggtttggaaaggtacatttttttttcatgtcttattcatagtttcattattaacttacagagcaactggaatgaaattgttgataactttgatgatatgaatttaaaggagtcccttcttcgaggcatctatgcatatggttttgagaagccttcagctattcagcaaagagctattatcccttgtattaaaggtaaaactctattgccgttaggataagtgctctgcacatacatggaccggaaaagggaaagtgacctttggggaagtactagcacctgattgtattccttaaagtgaaatgatggcaaatcatctttcgggactgacctgaaatgaagagaatactcttgctgatcacttatgtgggcgtaactttatgttccaactggaatacatggtgtatagtagaagtaacgatttgatgtgggatcggtaaatatgtatcctactttttttagggtatgatgtgattgctcaagctcagtcaggtactggcaagacagccacatttgctatttccatcctgcaacagttggagattgagttcaaggagacccaagcactagtattggcccccaccagagaactggctcaacaggtattgatagtgtagttcaaaaaactgcttgcgtgttgcataggtttcaggtttcacaactgtgaagaatttaaaattaaaacgtataaattgtcctagtcctgtgagagcccccattttaattgtccatgcatgcagggagttttgttgaaagctaagcaggaaatgggtattgtaggtatgactttagggattgattgcacctcaacagttaaacccatAGGTTTCTCAGGGTGGATTATATCTGTACCCATTTAAAATTTGGAATGCAGTTGTGCAACATGAAAACTGCAGTGACATGTTAAACATTTGACTGTCTCAGTAGTTTGTGATGCATCTGTTGCATGCTATGTTTTCAAAGCACTGCTATATTGGCTTTGAAGTAATACTTCCTGATGAAGCTGCAAGCTTTATTCG
This mutation is a 995 bp deletion beginning at Chromosome 16 position 23,108,486 bp and ending after 23,109,480 bp (GRCm38/mm10).
Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
66408 | GTC CAA GTC CCA AGC TGT TG | Common | ||||
66409 | AGC CAC GCA GAG ACC CTT A | Wild type Reverse | ||||
66410 | TGC ACA ACT GCA TTC CAA A | Mutant Reverse | ||||
66411 | Fluorophore-1 | CAG CAG AGC AGG TTT CTC AGG G | Quencher-1 | MUT Probe | ||
66441 | Fluorophore-2 | TTC CAC CCC GTA CCT CAT T | Quencher-2 | WT Probe |