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>chr7:79460564-79460674 111bp CGGATGCGTTTTCTCGATAC CTGCTGGGTCTTGTGCTTTC
Mutant = T, T
Wild type = C, C
SEQ
gctggcccctgtgttgcagccttccctcccctgcccttccttcagGACTCCCGGATGCGTTTTCTCGATACTATGAGCATGCACATGGCCATCTCGGGGCTGAGCAGCTT(c/t)CAG(c/t)GCAGCCTGtggatgGGAGCCAAGCAGGGAAAGCACAAGACCCAGCAGTCCACAAAGCGAGGGCAGAAGTCCCCGAGGAAAGCCAATGGTCCAGCGgtgagtgt
Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
64093 | CGG ATG CGT TTT CTC GAT AC | Forward | A | |||
64094 | CTG CTG GGT CTT GTG CTT TC | Reverse | A | |||
64095 | Fluorophore-1 | AGC TTC CAG CGC AGC | Quencher-1 | WT Probe | ||
64096 | Fluorophore-2 | CAG CTT TCA GTG CAG CCT | Quencher-2 | MUT Probe |
Component | Final Concentration |
---|---|
Kapa Probe Fast QPCR | 1.00 X |
ddH2O | |
64093 | 0.40 uM |
64094 | 0.40 uM |
Wt Probe | 0.15 uM |
Mutant Probe | 0.15 uM |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 95.0 | -- | |
2 | 95.0 | -- | |
3 | 60.0 | -- | |
4 | -- | repeat steps 2-3 for 40 cycles | |
5 | 40.0 | -- | Forever |