For in-depth product & services help, ask our
Technical Information Scientists
Mutant = GTTC
Wild type = ACAG
>chr2:181113013-181113102 90bp CTGGAAGCTCTTGGGATCG CTTGAAGTCATGGTAAACAGCAG
The For primer (primer 54043) anneals over the nucleotide sequence containing mouse genomic variations rs27642401 and the Rev primer (primer 54044) anneals over the nucleotide sequence containing mouse genomic variation rs227134979.
ATCTTGCGGATGATCCGTATGGACCGGAGGGGTGGCACCTGGAAGCTCTTGGGATCGGTAGTCTACGCTC(a/g)(c/t)(a/t)(g/c)CAAGGTGAGCAGCACTGACCCAGCCTGGAGCCTGCTGTTTACCATGACTTCAAGGCTCCAGGCTGCTCTA
Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
53556 | Fluorophore-1 | CTC ACA GCA AGG TGA GCA GC | Quencher-1 | WT Probe | ||
53557 | Fluorophore-2 | CTC GTT CCA AGG TGA GCA GC | Quencher-2 | MUT Probe | ||
54043 | CTG GAA GCT CTT GGG ATC G | Forward | A | |||
54044 | CTT GAA GTC ATG GTA AAC AGC AG | Reverse | A |
Component | Final Concentration |
---|---|
Kapa Probe Fast QPCR | 1.00 X |
ddH2O | |
54043 | 0.40 uM |
54044 | 0.40 uM |
Wt Probe | 0.15 uM |
Mutant Probe | 0.15 uM |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 95.0 | -- | |
2 | 95.0 | -- | |
3 | 60.0 | -- | |
4 | -- | repeat steps 2-3 for 40 cycles | |
5 | 40.0 | -- | Forever |