For in-depth product & services help, ask our
Technical Information Scientists
Taqman qPCR protocols are run on a real time PCR instrument. Use an appropriate instrument specific Fluorophore/Quencher combination.
Mutant= 84 bp
Wild Type = 96 bp
>chr6:3324616-3324711 96bp ACCAACTTTCTCCAAAAGAACA TCATGAACGAATAAAAGTTTTCAC
Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
44744 | ACC AAC TTT CTC CAA AAG AAC A | Wild type Forward | A | |||
44745 | TCA TGA ACG AAT AAA AGT TTT CAC | Common | A | |||
44746 | Fluorophore-1 | CTT TGA GGC CAA ACT ACA GGA | Quencher-1 | WT Probe | ||
44747 | GCA AAG TTA GCA AAT AGC ATT TCA | Mutant Forward | A | |||
44748 | Fluorophore-2 | AAC TTT CTC CAA GGA GCA TAA GAA | Quencher-2 | MUT Probe |
Component | Final Concentration |
---|---|
Kapa Probe Fast QPCR | 1.00 X |
ddH2O | |
44744 | 0.40 uM |
44745 | 0.40 uM |
44747 | 0.40 uM |
Wt Probe | 0.15 uM |
Mutant Probe | 0.15 uM |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 95.0 | -- | |
2 | 95.0 | -- | |
3 | 60.0 | -- | |
4 | -- | repeat steps 2-3 for 40 cycles | |
5 | 40.0 | -- | Forever |