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Mutant =G/G
Heterozygote = A/G
Wild type = A/A
chrX:136834117+136834201 85bp CTTATGAAGTTTACTCTGGCTGCT GTTGGAGCCACAAACCTTG
SEQ
Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
52184 | CTT ATG AAG TTT ACT CTG GCT GCT | Forward | A | |||
52185 | GTT GGA GCC ACA AAC CTT G | Reverse | A | |||
52186 | Fluorophore-1 | ATG TAT CTT AGG TGT TCT CCC ATG G | Quencher-1 | WT Probe | ||
52187 | Fluorophore-2 | ATG TAT CTT GGG TGT TCT CCC ATG | Quencher-2 | MUT Probe |
Component | Final Concentration |
---|---|
Kapa Probe Fast QPCR | 1.00 X |
ddH2O | |
52184 | 0.40 uM |
52185 | 0.40 uM |
Wt Probe | 0.15 uM |
Mutant Probe | 0.15 uM |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 95.0 | -- | |
2 | 95.0 | -- | |
3 | 60.0 | -- | |
4 | -- | repeat steps 2-3 for 40 cycles | |
5 | 40.0 | -- | Forever |