My primary research focuses on studying various cell types especially cancer cells using single-cell technology. Separately, I am also interested in understanding the impact of TET2 mutation in B cells functions and malignancy.
During my PhD I focused on the development of bioinformatics methods for single-cell genome sequence data analysis and subsequently the application of these tools to a diversity of cell types such as cancer and embryo samples and also rare cells like disseminated tumor cells, to understand genetic heterogeneity in primary normal and diseased tissues. In addition, I was also involved in a novel method to study the genome and the transcriptome of same single cell in parallel.
The simultaneous sequencing of a single cell's genome and transcriptome offers a powerful means to dissect genetic variation and its effect on gene expression. Here we describe G&T-seq, a method for separating and sequencing genomic DNA and full-length mRNA from single cells. By applying G&T-seq to over 220 single cells from mice and humans, we discovered cellular properties that could not be inferred from DNA or RNA sequencing alone.