I study the implication of myeloid cells, particularly antigen-presenting cells (APCs), in antiviral immune response.
My work is focused on implementing in vitro models in order to explore the molecular/cellular mechanisms of the immune system against respiratory virus. To this end, we are using organoids to expand lung progenitor cells from primary lung tissues to subsequently derive air-liquid interface (ALI) cultures. ALI cultures are then infected with influenza or SARS-CoV-2 in the presence or absence of immune cells. Responses to infection of both epithelial cells and immune cells are analyzed by flow cytometry, immunofluorescence and RNA-Seq. Those approaches will enable us to determine molecular mechanisms underlying alterations in the innate immune cells at epithelial barriers.