Did you know that CRISPR engineering can sometimes yield more than the allele you originally intended? Depending on timing, efficiency, and specificity of the Cas9 enzyme, resulting modifications in founder animals can be diverse, called mosaicism. Learn about more about mosaicism, how to resolve it, and other CRISPR basics in this 3 minute video short.
Do you need to generate new mouse models within 3-4 months instead of 1-1.5 years? Revolutionary CRISPR/Cas technology enables rapid, economical model generation through precise genome editing. During this webinar, we will help you understand the potential of CRISPR/Cas to accelerate your research.
CRISPR/Cas9 techniques are quickly growing in popularity for the generation of new mouse models for research. Though the approach is relatively simple, many researchers struggle with understanding the complexities associated with the diverse alleles that can be generated, how to deal with mosaicism, and how to move from a single founder mouse to larger cohorts for phenotype characterization.
Dr. Haoyi Wang shares how CRISPR/Cas has been successful in generating mice with knock-in alleles, conditional as well as multiple mutations in a single generation.