CLN5 W26X REV/REV

Product Code: JIPSC1342

Background

Genetics

Gene

CLN5

Condition

Neuronal ceroid lipofuscinosis_ late infantile

Allele Charactertistics

Autosomal Recessive

View Information in ClinVar

Derived from Cell Line

JIPSC1158_CLN5_W26X_SNV/SNV

Genomic Features

Genomic Coordinate (GRCh38)

chr13:76992176 (+)

dbSNP

rs104894385

Genomic Sequence

CGCGGGCGCGGCTCGGGGACGCGCTTCCTG[G/A]TGCTGGGCCCTGGCGCTGCTTTGGCTCGCG
View With Genome Browser

Gene Editing Reagents

Nuclease

SpCas9

Protospacer Sequence

GGGACGCGCTTCCTGATGCT

Donor Template

GGGGATGCCCGAGACCCGGGACCAGCCCGGAACCACCGCGAGCCAAAGCAGCGCCAGGGCCCAGCACCAGGAAGCGCGTCCCCGAGCCGCGCCCGCGCCC

DNA Primers for Sequence Validation

PCR Forward

GACCAAAGCACCTTCCTGGA

PCR Reverse

CCCATCCCCCATCGTCAAC

Sanger Sequencing Primer