Guide: Improved In Vitro Fertilization via Zona Thinning

Mouse In Vitro Fertilization (IVF) can be used to rapidly expand mouse lines from a few males that carry the desired genotype or to maintain strains with poor breeding efficiency. This guide describes a detailed method using acidified Tyrode solution to open the zona pellucida, and increase the fertilization capacity of frozen mouse sperm. The embryos generated are suitable for transfer into pseudopregnant recipient females, culture or cryopreservation.

Inside this guide you will find:

  • How to use Tyorde solution to optimize the fertilization rate when using frozen sperm
  • List of materials and equipment needed for IVF
  • A step-by-step method to generate 2-cell embryos

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