Visit our booth to learn about our model generation services,
transgenic mouse models, breeding services,
genotyping services, and more.
Genome editing with CRISPR/Cas9 enables the generation of new mouse models with unprecedented speed and simplicity. The Jackson Laboratory was an early adopter of CRISPR/Cas9 technology for mouse model development, and in this brief guide we describe the technology and lessons learned to generate knockouts and knockins in more than a dozen different genetic backgrounds.
JAX MGS utilized a short oligo-mediated knockin approach to generate a small mouse model panel in which each model carries a mouse allele that has been modified to carry a different, specified amino acid at key orthologous positions identified in the FALS patient analysis.
Dr. Haoyi Wang shares how CRISPR/Cas has been successful in generating mice with knock-in alleles, conditional as well as multiple mutations in a single generation.
To support a collaboration between Dr. Cat Lutz of The Jackson Laboratory and the Friedrich’s Ataxia Research Alliance, our Model Generation Services (MGS) team undertook a project to create a conditional knockout allele of the Frataxin (Fxn) gene using the Cre-loxP Recombination System.