Embryo Cryopreservation

JAX has 40 years' experience in cryopreserving embryos. Leverage our expertise and protect the longevity of your research with our Embryo Cryopreservation Service.

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Proven successful recovery using Embryo Cryopreservation

JAX has successfully refined the embryo cryopreservation technique for more than 30 years to recover mice quickly and reliably. In addition to being ideal for strains with multiple mutations, this method also allows you to:

  • Preserve the genetic background of your strain upon recovery
  • Recover the precise genotype of your genetic mutation or transgene, compared to Sperm Cryopreservation

Custom embryo cryopreservation

For strains with unique genetic backgrounds or those that require homozygous cryopreservation.

Key benefits

  • Preserves the homozygous characteristics of your mouse strain
  • Ideal for strains with multiple genetic mutations
  • Preserves unique genetic backgrounds

Get started using our custom embryo cryopreservation form

Details

  • Best suited for inbreds, homozygotes, or strains with multiple genetic mutations
  • Total number of embryos cryopreserved determined by a test cryopreservation and recovery
  • Typical timeframe of four to six months
  • Requires four to six males and six to ten females from your colony

Pricing

By quotation only. Please contact JAX® Services for more information.

  • Email jaxservices@jax.org
  • Call 1-800-422-6423 (US, Canada & Puerto Rico) or 1-207-288-5845 (from any location)

Cryo storage

JAX maintains over 14,000 strains in a cryopreserved state. All tanks are auto filled, auto alarmed, routinely inspected and under 24/7 surveillance in secure facilities. We divide samples among multiple tanks in multiple locations for extra safety and security.

Benefits

  • Ideal for safely storing large numbers of your cryopreserved strains off-site
  • Storage included with all our cryopreservation services
  • No need to invest in and maintain infrastructure—including databases—to keep mouse germplasm safely cryopreserved
  • Germplasm can be quickly recovered to produce specified and opportunistic pathogen free (SOPF) mice
  • Ideal for transgenic cores and investigators with many cryopreserved samples
  • Great 'back up' plan for disaster recovery

Capabilities

  • Safe storage of your cryopreserved mouse sperm, embryos, or ES cells
  • Additional years of storage available

Embryo Cryo Details

  • Available for strains on C57BL/6J, FVB/NJ, DBA/2J, BALB/cByJ, or NOD/ShiLtJ backgrounds

  • At least 250 two-cell embryos/strain cryopreserved in 10 straws (25 embryos/straw)
  • Cryopreserved embryos are heterozygous (if homozygous males are supplied) or heterozygous and wild-type (if heterozygous males are supplied)
  • Quality control: systematic checks throughout cryopreservation process
    • Test thaws of control embryos
    • IVF of control embryos to validate the media and environmental conditions
    • Attempt to recover live born mice (except for strains on C57BL/6J backgrounds)