Previous First Look Strains

Strains Added in July 2023

Stock Number	Name	Common Name	Description
037952	STOCK Igs7^{tm3(tetO-GCaMP8s,CAG-tTA2)Genie}/J	TIGRE2-jGCaMP8s-IRES-tTA2-WPRE	TIGRE2-jGCaMP8s-IRES-tTA2-WPRE knock-in mice co-express the jGCaMP8s genetically encoded calcium indicator (GECI) and a tetracycline controlled transactivator (tTA2) in a Cre recombinase-dependent manner from the intergenic Igs7 (TIGRE) locus. An internal ribosome entry site (IRES) introduced upstream of tTA ameliorates previously reported tTA overexpression toxicity issues. jGCaMP8s is a fast rise, medium decay green fluorescent-based GECI with improved sensitivity and faster kinetics.
020074	C57BL/6-Tg(Dbh-cre/ERT2)198.1Hroh/J	DBH	DBH^CreERT2 transgenic mice express a tamoxifen-inducible Cre recombinase directed by mouse Dbh (dopamine beta hydroxylase) promoter elements. When induced, Cre recombinase activity is observed in noradrenergic cells.
038103	C57BL/6J-Trem2^{em3(TREM2)Aduci}/J	hTREM2-KI	hTREM2_KI mice contain wildtype human TREM2 sequence replacing the corresponding mouse sequence. These mice serve as a control for mice carrying the hTREM2R47H_KI* allele, which express human TREM2 with a R47H amino acid substitution within the extracellular Ig-like domain, and may be useful as a control in pre-clinical studies of Alzheimer's disease (AD).
038249	C57BL/6-Gt(ROSA)26Sor^{tm1(CAG-Gria2)Bli}*/J	Rosa26-stop-GluA2	The Rosa26-stop^flox-GluA2^mut knock-in mice harbor a floxed stop cassette followed by two point mutations (L504Y and R845A) of the glutamate receptor, ionotropic, AMPA2 (alpha 2), gene (Gria2), knocked-into the Gt(ROSA)26Sor locus. These mice may be useful for studying the role of the Gria2 in cre-expressing tissues, specifically as it relates to neurodevelopmental disorders and neurodegenerative diseases.
034855	B6.129(CBA)-Fto^tm1.2Pzg/J	Fto<#Δ>	Fto^#Δ knockout mice have exon 3 deleted in the fat mass and obesity associated (Fto) gene. Homozygotes exhibit immediate growth retardation and 50% of homozygous mice die with in 2 weeks. These mice may be useful for studying susceptibility to diet induced obesity, growth rate and metabolism. Of note, Fto conditional knockout mice (loxP-flanked exon 3) are distributed as B6.129-Fto^tm1.1Pzg/YxtJ (Stock No. 027830).
037719	STOCK Igs7^{tm2(tetO-GCaMP8s,CAG-tTA2)Genie}/J	TIGRE2‐jGCaMP8s‐IRES‐tTA2	TIGRE2-jGCaMP8s-IRES-tTA2 knock-in mice co-express the jGCaMP8s genetically encoded calcium indicator (GECI) and a tetracycline controlled tetracycline controlled transactivator (tTA2) in a Cre recombinase-dependent manner from the intergenic Igs7 (TIGRE) locus. The addition of an internal ribosome entry site (IRES) introduced upstream of tTA and the omission of a WPRE enhancer element downstream ameliorate previously reported tTA overexpression toxicity issues. jGCaMP8s is a fast rise, medium decay green fluorescent-based GECI with improved sensitivity and faster kinetics.
037718	STOCK Igs7^{tm1(tetO-GCaMP8m,CAG-tTA2)Genie}/J	TIGRE2‐jGCaMP8m‐IRES‐tTA2‐WPRE	TIGRE2-jGCaMP8m-IRES-tTA2-WPRE knock-in mice co-express the jGCaMP8m genetically encoded calcium indicator (GECI) and a tetracycline controlled transactivator (tTA2) in a Cre recombinase-dependent manner from the intergenic Igs7 (TIGRE) locus. An internal ribosome entry site (IRES) introduced upstream of tTA ameliorates previously reported tTA overexpression toxicity issues. jGCaMP8m is noted for its faster decay rate and offers a useful compromise between sensitivity and kinetics.
038372	C57BL/6J-Del(Gm41148-Gm35360)14Zbch/J	leene-KO	Leene-KO mice carry a 47 kb deletion in mouse chromosome 14 (mm39 chr14:48,019,230–48,066,441) which corresponds to the human long intergenic non-protein coding RNA 520 locus (LINC00520). This strain may be useful for studying endothelial homeostasis and eNOS regulation in metabolic disorders, cardiovascular diseases and some cancers.
019062	B6.C3-Pla2g6^m1J/CxRwb		This spontaneous mutation provides a model for infantile neuroaxonal dystrophy (INAD). Axonal spheroids are evident in the brain stem, spinal cord and peripheral axons of affected mice upon histological examination.
035931	B6N(129S4)-Pex10^{tm1c(EUCOMM)Wtsi}/LutzyJ	Pex10	Pex10^flox mice have loxP sites flanking exon 3 of the peroxisomal biogenesis factor 10 (Pex10) gene. These mice are useful for studying peroxisome import.
038457	STOCK Atp1a3^tm1.1Mika/LutzyMmjax	Atp1a3 , Matoub , Matb+/-	Atp1a3^E815K knock-in mice carry a E815K amino acid substitution of the ATPase, Na+/K+ transporting, alpha 3 polypeptide or Matb. The E815K mutation is lethal in homozygotes. These mice may be useful in studying alternating hemiplegia of childhood (AHC). Of note, Atp1a3^E815K mice on the C57BL/6 congenic background are distributed as B6.129(Cg)-Atp1a3^tm1.1Mika/Mmjax (MMRRC Stock No. 069591). The C57BL/6 congenic background has a more severe phenotype.
038294	B6C3-Atp1a3^em3Lutzy/Mmjax	Atp1a3	Atp1a3^D801N is a CRISPR/Cas9 generated mutant of the ATPase, Na+/K+ transporting, alpha 3 polypeptide gene carrying the D801N point mutation. These mice may be useful in studying dystonia-12, alternating hemiplegia of childhood-2, and cerebellar ataxia, areflexia, pes cavus, optic atrophy, and sensorineural hearing loss (CAPOS). The MMRRC distributes the Atp1a3^D801Nmutation (MMRRC Stock No. 066968) on a C57BL/6J background.
038293	FVB.Cg-Pde6b⁺ Tyr^c-ch Ccdc198^em1Iad/J	FAME	Knock-out mice have a double stop codon inserted following the initiator ATG site of the Ccdc198 (coiled-coil domain containing 198 or FAME) locus resulting in a null mutation. Mice homozygous for the mutation develop decreased ferritin levels and high amounts of albumin in urine. This strain maybe useful in studies examining iron exchange, metabolite excretion and energy expenditure.
038235	C57BL/6J-Eln^em1Mech/JwagJ	Eln	Eln^f mice possess loxP sites flanking exons 4-29 of the elastin (Eln) gene. These mice may be useful in generating conditional mutations for studying diseases such as atherosclerosis, aortic stenosis and emphysema.
037822	129S-Kcnab2^tm1Kmc/NystJ	Kvβ2-null	Kvβ2 knock-out mice have exons 7-9 of the Kcnab2 (potassium voltage-gated channel, shaker-related subfamily, beta member 2) gene replaced by a neomycin cassette. Homozygous null mice have a reduced lifespan, seizures, and cold swim-induced tremors. This strain may be useful for studies involving potassium channel regulation and chromosome 1p36 deletion syndrome.
037823	129S-Kcnab2^tm2Mes/NystJ	Kvβ2-Y90F	Kvβ2-Y90F knock-in mice have a tyrosine to phenylalanine substitution at position 90 (Y90F). of the Kcnab2 (potassium voltage-gated channel, shaker-related subfamily, beta member 2) gene. This strain may be useful for studies involving potassium channel regulation.
038055	C57BL/6J-Tg(tetO-Pum2)1Jtm/J	TRE-Pum2	TRE-Pum2 transgenic mice enable conditional, tetracycline-dependent expression of FLAG-tagged mouse Pum2 (pumilio RNA-binding family member 2) from a tetO promoter when combined with a tTA or rtTA mutation. This strain has been useful in studies of Norad, a long noncoding RNA (lncRNA), whose deletion is associated with premature aging phenotypes (see Stock No. 038054).
038054	C57BL/6J-Norad^em1Jtm/J	Norad-KO , Norad<->	Norad-KO mice carry a CRISPR/Cas9-generated knock-out of mouse Norad (non-coding RNA activated by DNA damage), a long noncoding RNA (lncRNA). Deletion of NORAD in mice results in genomic instability and mitochondrial dysfunction, leading to a dramatic multi-system degenerative phenotype resembling premature aging.
037888	B6.129S1-Best1^tm1Web/J	Best1<->	Homozygous Best1 (bestrophin 1) knock-out mice on a C57BL/6J background demonstrate a subfertility phenotype due to enhanced abnormal sperm morphology related to impaired volume regulation.
038186	C57BL/6-Klrg1^em1Lbro/J	Klrg1<->	Klrg1^- mice express a CRISPR-made deletion of exon 3 the killer cell lectin-like receptor subfamily G, member 1 (Klrg1) gene. These mice may be useful for studying the role of KLRG1 in apoptosis, innate and adaptive immunity and cancer biology.
033122	C57BL/6J-Il2rg^em3Lutzy/J	B6J.Il2rg KO	B6J.Il2rg KO mice have a CRISPR/Cas9-generated deletion of exon 3 of the interleukin 2 receptor, gamma chain locus (Il2rg). These B6J.Il2rg KO mice may be useful for studying NK cell development, innate immunity, T and B cell deficiency.
038422	B6;129S-Npr2^tm1.2Laj/Mmjax	Npr2-7E-cKI	Of note, mice constitutively expressing the 7E mutations are available as B6;129-Npr2^tm1.2Laj/Mmjax (MMRRC Stock No. 071312). Conditional Npr2-7E cKI mice have a loxP-flanked EGFP reporter upstream of 7 glutamate substitutions (7E) in exons 8 and 9 of the Npr2 (natriuretic peptide receptor 2) gene. The 7E mutations prevent NPR2 dephosphorylation and inactivation. These floxed mice may be used as a tool for understanding signaling in mouse preovulatory follicles and bone growth regulation.
038421	C57BL/6J-Npr2^em1Laj/Mmjax	HA-NPR2	HA-NPR2 knock-in mice carry a hemagluttin (HA) tag inserted into the N-terminal of the Npr2 (natriuretic peptide receptor 2) gene. This strain may be used as a tool for understanding signaling in mouse preovulatory follicles and bone growth regulation.
038420	C57BL/6J-Lhcgr^em1Laj/Mmjax	HA-LHR	HA-LHR knock-in mice carry a hemagluttin (HA) tag inserted into the N-terminal of the Lhcgr (luteinizing hormone/choriogonadotropin receptor, also called LHR) gene. This strain may be used as a tool for understanding signaling in mouse preovulatory follicles.
031489	B6;129S-Npr2^tm1.1Laj/Mmjax	Npr2-7E	Npr2-7E mice have 7 glutamate substitutions (7E) in exons 8 and 9 of the Npr2 (natriuretic peptide receptor 2) gene. The 7E mutations prevent NPR2 dephosphorylation and inactivation. Homozygous mice develop longer and stronger bones. These mice may be used for research in signaling in mouse preovulatory follicles and bone growth regulation. Of note, mice conditionally expressing the 7E mutations are available as B6;129-Npr2^tm1.2Laj/Mmjax (MMRRC Stock No. 071310).
037954	B6.Cg-Gt(ROSA)26Sor^{tm4(CAG-cas9/GAG-POL,-mNeonGreen)Tyj} Trp53^tm1Brn*/TyjJ	Rosa26;Trp53	Rosa26^PE2 Trp53^flox knock-in mice allow cre-inducible expression of a prime editor (PE2) allele consisting of a human optimized cas9 fused to a MMLV-RT (Moloney murine leukemia virus reverse transcriptase, GAG-POL) and an mNeonGreen reporter in the context of a cre-mediated Trp53 null allele. The Rosa26^PE2 Trp53^flox model facilitates in vivo prime editing for precise editing of transition and transversion single nucleotide variants (SNVs) and indels in murine tissues and in vitro prime editing in organoids and cell lines derived from various tissues. This strain may be used to accelerate preclinical functional studies of cancer-associated alleles and complex genetic combinations. Of note, a strain carrying only the Rosa26^PE2 allele is distributed as B6.Cg-Gt(ROSA)26Sor^{tm4(CAG-cas9/GAG-POL,-mNeonGreen)Tyj}*/J (Stock No. 037953).
037953	B6.Cg-Gt(ROSA)26Sor^{tm4(CAG-cas9/GAG-POL,-mNeonGreen)Tyj}*/J	Rosa26 , LSL PE2	Rosa26^PE2 knock-in mice allow cre-inducible expression of a prime editor (PE2) allele consisting of a human optimized cas9 fused to a MMLV-RT (Moloney murine leukemia virus reverse transcriptase, GAG-POL) and an mNeonGreen reporter. The Rosa26^PE2 model facilitates in vivo prime editing for precise editing of transition and transversion single nucleotide variants (SNVs) and indels in murine tissues and in vitro prime editing in organoids and cell lines derived from various tissues. This strain may be used to accelerate preclinical functional studies of cancer-associated alleles and complex genetic combinations. Of note, a strain carrying the Rosa26^PE2 allele and a floxed Trp53 allele is distributed as B6.Cg-Gt(ROSA)26Sor^{tm4(CAG-cas9/GAG-POL,-mNeonGreen)Tyj} Trp53^tm1Brn*/TyjJ (Stock No. 037954).

Strains Added in June 2023

Stock Number	Name	Common Name	Description
037394	C57BL/6-Ctnna3^em1Cgot/J	Ctnna3	Ctnna3^KO mice harbor a membrane associated eGFP construct inserted into exon 2 of the catenin (cadherin associated protein), alpha 3 gene (Ctnna3). These mice express eGFP-caax under the Ctnna3 promoter, while knocking-out endogenous Ctnna3 expression. These mice may be useful for studying localization and function of CTNNA3 at the subcellular level in the heart, brain and testes.
037502	C57BL/6NJ-Gaa^em2Jhng/J	GaaA>	Gaa^c.1935C>A mice carry a CRISPR/Cas9 generated aspartic acid to glutamic acid change at amino acid 645 (Asp645Glu) in the glucosidase, alpha, acid (Gaa) gene, making this strain useful when studying Infantile Onset Pompe Disease (IOPD).
037512	B6.Cg-Slc17a7^{tm1.1(cre)Hze}/J	Slc17a7-IRES2-Cre-D	Slc17a7-IRES2-Cre-D knock-in mice have Cre recombinase expression directed to Vglut1-expressing cells, without disrupting endogenous vesicular glutamate transporter 1 expression. These mice may be useful for studying glutamatergic synaptic vesicle trafficking and vesicle-bound, sodium-dependent phosphate transportation. Stock No. 037512 is a C57BL/6J-congenic Slc17a7-IRES2-Cre-D knock-in mouse line. Of note, the same allele is also available on a B6;129S genetic background as Stock No. 023527.
037592	B6;129S4-Cdh8^{tm2c(KOMP)Wtsi}/DlbeJ	Cdh8	Cdh8^FL/FL mice have loxP sites flanking exon 3 of the cadherin 8 gene, making them useful for studying synaptic adhesion, axon outgrowth and guidance.
037627	STOCK Nrp1^tm1.1Chgu/J	Nrp1	Nrp1^VEGF- carry a D320K mutation in exon 6 of the mouse neuropilin 1 (Nrp1) gene that selectively disrupts binding to VEGF (vascular endothelial growth factor), a key regulator of angiogenesis.
037647	C57BL/6-Ch25h^tm1.1Syfu/J	Ch25h floxed	Ch25h floxed mice possess loxP sites flanking the only exon of the cholesterol 25-hydroxylase (Ch25h) gene. These mice may be useful for studying the role of Ch25h in lipid metabolism, inflammation, and innate immune responses.
037658	C57BL/6-Tg(Myh11-cre/ERT2)F31Gko/J	Myh11-CreER-RAD	Myh11-CreER^T2-RAD transgenic mice allow sufficient Myh11-driven tamoxifen-inducible cre expression in smooth muscle cells (SMCs) in the brachiocephalic artery (BCA) and aorta. This transgene integrated into mouse Chromosome 2 in a region devoid of other genes [Chr2:178,826,578-178,826,802 (GRCm39)]. Of note, Stock No. 019079 and Stock No. 036935 each contain a similar BAC transgene inserted on the Y chromosome and X Chromosome, respectively.
037673	B6.129S-Atxn1^em3Hzo/J	Atxn1<154Q[V591A;S602D]/2Q>	Atxn1^{154Q[V591A;S602D]/2Q} mice carry a 154Q repeat as well as V591A and S602D (mouse V566A and S577D) mutations in the mouse Atxn1 (ataxin 1) gene. Protein interactions with transcriptional repressor CIC (capicua) are disrupted. Heterozygotes display phenotypes similar to the Atxn1^154Q/2Q model of spinocerebellar ataxia type 1 (SCA1) (Stock No. 005601), however they are less severe.
037674	B6.129S7-Atxn1^em4Hzo/J	Atxn1<2Q[V591A;S602D]/2Q>	Atxn1^{2Q[V591A;S602D]/2Q} mice carry CRISPR/Cas9-generated V591A and S602D (mouse V566A and S577D) mutations in the wildtype Atxn1^2Q (ataxin 1) allele. Homozygotes demonstrate no ATXN1 binding to CIC (capicua). This strain has been useful in studies of spinocerebellar ataxia type 1 (SCA1), and acts as a potential control for Atxn1^{154Q[V591A;S602D]/2Q} mice (Stock No. 037673). These mice will also be useful for the study of ATXN1-CIC protein interactions, independent of SCA1 disease.
037700	B6.129P2-Gata2^tm1.1Dzk/J	Gata2Venus	Gata2Venus knock-in mice have an IRES and a Venus sequence inserted in the 3' UTR of the Gata2 gene. These mice are useful for studying real-time transcription and protein expression of Gata2 in vivo without affecting hematopoietic development or function.
037713	STOCK Igdmr^em1Yste/J	IG-CGI	IG-CGI^f mice possess loxP sites flanking the CpG island (IG-CGI) located at the 5prime portion of an intergenic differentially methylated region (IG-DMR), which controls imprinting across the Dlk1-Dio3 domain. This strain may be useful for studying DNA methylation and genomic imprinting.
037723	STOCK Dmrt1^em1Zark/J	Dmrt1	Dmrt1^R111G knock-in mice express the R109G (aspargine to glycine amino acid substitution in the Dmrt1 (doublesex and mab-3 related transcription factor 1) locus. The R109G mutation is equivalent to the R111G mutation in humans. Male heterozygotes have gonadal dysgenesis with partial feminization of the transcriptome and are infertile. These mice may be useful in studies of male germ cells development as well as dominant complete gonadal dysgenesis and 46,XY sex reversal.
037781	C57BL/6J-Tg(Prnp-CHCHD10)U34Dkan/J	CHCHD10-WT	CHCHD10-WT mice harbor the human wildtype coiled-coil-helix-coiled-coil-helix domain containing 10 (CHCHD10) transgene driven by the endogenous prion (Prnp) promoter. These mice may be useful for studying amyotrophic lateral sclerosis (ALS), frontotemporal dementia (FTD) and mitochondrial myopathies. These CHCHD10-WT mice may be used as controls for CHCHD10-R15L (Stock No. 037782) and CHCHD10-S59L (Stock No. 037783) transgenic models.
037782	C57BL/6J-Tg(Prnp-CHCHD10*R15L)U2Dkan/J	CHCHD10-R15L	CHCHD10-R15L mice harbor the human R15L mutation in the coiled-coil-helix-coiled-coil-helix domain containing 10 (CHCHD10) transgene driven by the endogenous prion (Prnp) promoter. These mice may be useful for studying amyotrophic lateral sclerosis (ALS), frontotemporal dementia (FTD) and mitochondrial myopathies. The donating laboratory has made multiple Prnp-CHCHD10 transgenic lines available: CHCHD10-WT (Stock No. 037781), CHCHD10-R15L (Stock No. 037782) and CHCHD10-S59L (Stock No. 037783).
037783	C57BL/6J-Tg(Prnp-CHCHD10*S59L)U1Dkan/J	CHCHD10-S59L	CHCHD10-S59L mice harbor the human S59L mutation in the coiled-coil-helix-coiled-coil-helix domain containing 10 (CHCHD10) transgene driven by the endogenous prion (Prnp) promoter. These mice may be useful for studying amyotrophic lateral sclerosis (ALS), frontotemporal dementia (FTD) and mitochondrial myopathies. The donating laboratory has made multiple Prnp-CHCHD10 transgenic lines available: CHCHD10-WT (Stock No. 037781), CHCHD10-R15L (Stock No. 037782) and CHCHD10-S59L (Stock No. 037783).
037787	C57BL/6J-Kcnma1^em1Alme/J	KCNMA1-N999S	KCNMA1-N999S knock-in mice express a gain-of-function (GOF) asparagine to serine substitution at position 999 of the potassium large conductance calcium-activated channel, subfamily M, alpha member 1 gene. Heterozygous mice exhibit lowered seizure thresholds, hypokinetic stress-induced paroxysmal dyskinesia (episodes of immobility), locomotor dysfunction, and increased BK channel activity. These mice may be useful in studies involving KCNMA1-linked channelopathy. Additional strains with patient-associated KCNMA1-linked channelopathy mutations include KCNMA1-D434G (Stock No. 037788) and KCNMA1-H444Q (Stock No. 037789).
037788	C57BL/6J-Kcnma1^em2Alme/J	KCNMA1-D434G	KCNMA1-D434G knock-in mice express a gain-of-function (GOF) autosomal dominant aspartic acid to glycine substitution at position 434 of the potassium large conductance calcium-activated channel, subfamily M, alpha member 1) gene. Mice exhibit decreased seizure thresholds, hypokinetic stress-induced paroxysmal dyskinesia (episodes of immobility), and locomotor dysfunction. These mice may be useful in studies involving KCNMA1-linked channelopathy. Additional strains with patient-associated PKND3 mutations include KCNMA1-N999S (Stock No. 037787) and KCNMA1-H444Q (Stock No. 037789).
037789	C57BL/6-Kcnma1^em3Alme/J	KCNMA1-H444Q	KCNMA1-H444Q knock-in mice express a loss-of-function (LOF) histidine to glutamine substitution at position 444 of the potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (Kcnma1) gene. Mice exhibit locomotor dysfunction and hyperactive dyskinesia. These mice may be useful in studies involving KCNMA1-linked channelopathy. Additional strains with patient-associated PKND3 mutations include KCNMA1-N999S (Stock No. 037787) and KCNMA1-D434G (Stock No. 037788).
037790	B6N.Cg-Cd40^tm1.1Wgnr/HodeJ	CD40	CD40^fl/fl mice have loxP sites flanking exon 1 of the Cd40 gene. These mice may be useful when studying the progression of autoimmune diseases by CD40-driven effector programs in B cells and dendritic cells.
037797	C57BL/6J-Il33^tm1Cln/J	Il33	Il33^cherry knock-in/knock-out mice harbor an H2B-mCherry reporter cassette inserted between exons 4 and 5 of the interleukin 33 (Il33) locus. These mice may be useful for studying and tracking interleukin 33 activity.
037800	B6.129X1(Cg)-Nfkbia^tm1.1Pjc/J	IκBα	IκBα^M knock-in mice harbor mutations in two NF-κB binding sites (M1 and M2) and 4 NF-κB-like binding sites (M3-M6) in the promoter region of the nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, alpha gene (Nfkbia). These mice may be useful in studying the regulatory role of Nfkbia on NF-κB activity and autoimmune diseases.
037803	B6.Cg-H2-K1^b-em1Tyj/AjgrJ	KStrep	K^bStrep knock-in mice have an inverted StrepTagII, flanked by two pairs of inward-facing heterospecific Lox sites, inserted into intron 1 of the H2-K1 gene. Upon cre induction, recombination of the Lox sites results in the inversion of the StrepTagII into the correct transcriptional orientation. These mice may be useful for the selective purification of MHC-I peptide antigens from cell type(s) of interest from intact tissues.
037804	B6.Cg-Clec9a^{tm4.1(cre/ERT2)Crs}/J	Clec9a	Clec9a^CreERT2 knock-in mice have a P2A peptide fused to a cre/ERT2 sequence inserted into the C-terminal of the Clec9a gene. This strain is useful for dendritic cell lineage fate mapping and for studying adaptive immune responses to antigens in apoptotic and necrotic cells.
037805	B6.Cg-Clec9a^tm5.1Crs/J	Clec9a	Clec9a^tdTomato knock-in/knock-out mice have tdTomato with a BGH_pA inserted into the ATG in exon 1 of the Clec9a gene. This strain is useful for dendritic cell lineage fate mapping and for studying adaptive immune responses to antigens in apoptotic and necrotic cells.
037827	B6.129(Cg)-Ep300^tm1.1Kahn/J	p300 S89A	p300 S89A mice have a serine to alanine mutation (S89A) in exon 2 of the Ep300 gene. This mutation removes a highly conserved phosphorylation site at S89 making these mice useful when studying p300 signaling and its effects on intestinal homeostasis and repair.
037835	C57BL/6J-Kcnb1^em1Sesf/J	Kcnb1	R312H knock-in (KI) is a CRISPR/Cas9 generated mutant of the potassium voltage gated channel, Shab-related subfamily, member 1 (Kcnb1) gene carrying the R312H knock-in mutation. These mice may be useful for studying the role of Kcnb1 in neocortical growth and Developmental and Epileptic Encephalopathies (DEEs).
037836	B6(FVB)-Il1b^em1Mora/J	IL-1β	IL-1β^fl mice possess loxP sites flanking exon 4 of the Interleukin 1 beta (Il1b) gene. These mice may be useful in studying inflammation and autoimmune disease.
037843	C57BL/6-Agpat5^tm1Thor/J	Agpat5	Exon 3 of the mouse Agpat5 (1-acylglycerol-3-phosphate O-acyltransferase 5 (lysophosphatidic acid acyltransferase, epsilon) gene is flanked by lox2272/loxP sites in these Agpat5^flox mice. Cre recombinase-mediated excision of the floxed region results in a knock-out allele and expression of a tdTomato reporter useful in studies of agouti-related peptide neurons and their roles in insulin-induced hypoglycemia sensing and glucagon secretion.
037848	C57BL/6J-Kcnb1^em2Sesf/J	Kcnb1 null	The Kcnb1 null allele is a CRISPR/Cas9 generated mutant of the potassium voltage gated channel, Shab-related subfamily, member 1 (Kcnb1) gene resulting in expression of a non-functional 337 amino acids truncated KCNB1 protein. These mice may be useful for studying the role of Kcnb1 in neocortical growth and Developmental and Epileptic Encephalopathies (DEEs).
037864	STOCK Atp5if1^{tm1c(EUCOMM)Wtsi}/RtiJ	ATPIF1-floxed	Exon 3 of the Atp5if1 (ATPase 5 inhibitory factor 1) gene is flanked by loxP sites in this conditional mutant strain. These mice may be useful in Cre-lox studies of cardiac hypertrophy.
037882	C57BL/6J-Slc6a2^em1(cre)Lbrl/J	Slc6a2-p2a-Cre	CRISPR-generated Slc6a2-p2a-Cre knock-in mice express cre recombinase from the mouse solute carrier family 6 (neurotransmitter transporter, noradrenalin), member 2 (Slc6a2) promoter. This strain has been useful in studies of area postrema excitatory neurons as they relate to nausea-associated behaviors.
037894	B6.Cg-Kcnq2^em3Frk/Mmjax	Kcnq2-T274M	Kcnq2^em3Frk is a CRISPR/Cas9 generated knock-in mutant to the Kcnq2 (potassium voltage-gated channel, subfamily Q, member 2) gene carrying the T274M (ACC to ATG) and T277T (silent PAM deleter) mutations. These mice may be useful in studying epilepsy.
037912	C57BL/6-Gt(ROSA)26Sor^{tm1.1(CAG-Nfatc,-tdTomato)Znlab}*/Mmjax	iNFATuation	iNFATuation knock-in mice conditionally express a peptide inhibitor (VIVIT) and tdTomato reporter. VIVIT selectively inhibits calcineurin/NFATC1-4 (nuclear factor of activated T cells, cytoplasmic, calcineurin dependent) interaction. iNFATuation mice maybe used for studies involving NFAT inhibition in a cell-type specific manner.
037919	B6.129S4(FVB)-Nos3^tm2.1Plh/J	eNos S1176A	eNos S1176A knock-in mice harbor an amino acid substitution of serine to alanine in exon 26 of the nitric oxide synthase 3, endothelial cell (Nos3 or eNos) gene. These mice may be useful for studying the role of eNos in cardiovascular disease, hypertension, and metabolic syndrome. The donating laboratory also created a phosphomimetic eNos knock-in mouse line, S1176D (Stock No. 037920), which may be utilized as a positive control to the eNos S1176A knock-in mice.
037920	B6.129S4(FVB)-Nos3^tm3.1Plh/J	eNos S1176D	eNos S1176D knock-in mice harbor an amino acid substitution of serine to aspartate in exon 26 of the nitric oxide synthase 3, endothelial cell (eNos) gene. These mice may be useful for studying the role of eNos in cardiovascular disease, hypertension, and metabolic syndrome. The donating laboratory also created a nonphosphorylatable eNos knock-in mouse line, S1176A (Stock No. 037919), which may be utilized as a negative control to the eNos S1176D knock-in mice.
037930	B6.Cg-Grb10^tm1.1Esze/J	Grb10-H2B-Venus	Grb10^P2A-H2B-Venus knock-in mice have a bicistronic reporter replacing the stop codon of exon 18 of the Grb10 gene. Specifically, this vector includes (from 5' to 3') an in frame replacement of the endogenous Grb10 stop codon with a P2A-H2B-Venus non-gene disruptive reporter cassette. Endogenous Grb10 expression is as expected in the brain, with nuclear Venus expression in cells that express the imprinted Grb10 locus. These mice may be useful in studying Grb10 function and imprinted-expression patterns in cellular growth, metabolism, neurogenesis and social behavior.
037935	C57BL/6J-Grm3^tm1.1Conn/J	Grm3	Grm3^Fl floxed mice have loxP sites flanking exon 3 of the Grm3 gene. These mice may be useful when studying the role of mGlu₃ receptors in the regulation of synaptic plasticity and cognition.
037951	B6.129(Cg)-Wapl^tm1.1Kpfe/J	Wapl	Wapl^Flox mice possess loxP sites flanking the endogenous promoter and exon 2 of the WAPL cohesin release factor (Wapl) gene. The mutation results in a hypomorph with WAPL protein levels reduced by two-fold. The strain may be useful in studies of cohesion and cohesinopathies.
037966	C57BL/6J-Trpm4^em1Sthg/J	TRPM4	TRPM4^I1029M knock-in mice express a gain-of-function (GOF) isoleucine to methionine substitution at position 1029 of the transient receptor potential cation channel, subfamily M, member 4 (Trpm4) gene. Heterozygous mice treated with imiquimod develop a psoriasis-like dermatitis. These mice may be useful as a model for progressive systemic erythrokeratodermia (PDEK).
037966	C57BL/6J-Trpm4^em1Sthg/J	TRPM4	TRPM4^I1029M knock-in mice express a gain-of-function (GOF) isoleucine to methionine substitution at position 1029 of the transient receptor potential cation channel, subfamily M, member 4 (Trpm4) gene. Heterozygous mice treated with imiquimod develop a psoriasis-like dermatitis. These mice may be useful as a model for progressive systemic erythrokeratodermia (PDEK).
037967	C57BL/6J-Il1a^em1Tdk/J	Il1a-KO	The Il1a KO^{line 2} mice have a CRISPR/Cas9 generated deletion of exons 2-6 of the interleukin 1 alpha (Il1a) gene. These mice may be useful when studying inflammatory pathways, hematopoiesis and immunity.
037968	C57BL/6J-Ppara^em1Ngwu/J	PPARα HA knock-in	PPARα HA knock-in mice express an N-terminal HA-tag under direction of the endogenous Ppara promoter. This mouse strain could be useful for studying the regulatory mechanisms required to drive the energy-burning characteristics of brown adipocytes to counter obesity.
037969	C57BL/6J-Cebpa^em1Ngwu/J	C/EBPα HA knock-in	C/EBPα HA knock-in mice express an N-terminal HA-tag under direction of the endogenous Cebpa promoter. This mouse strain could be useful for studying the energy-burning characteristics of brown adipocytes, weight homeostasis, gluconeogenesis, and lipogenesis.
037970	C57BL/6J-Cebpb^em1Ngwu/J	C/EBPβ HA knock-in	C/EBPβ HA knock-in mice express an N-terminal HA-tag under direction of the endogenous Cebpb promoter. This mouse strain could be useful for studying the energy-burning characteristics of brown adipocytes, adipogenesis, and immune and inflammatory responses.
037971	C57BL/6J-Ppargc1a^em1Ngwu/J	PGC1α HisHA knock-in	PGC1α HisHA knock-in mice express a C-terminal HisHA tag immediately upstream of the stop codon of the Ppargc1a gene. This mouse strain could be useful for studying the energy-burning characteristics of brown adipocytes and the regulation of energy metabolism.
037972	B6;129-Tshz1^tm1(flpo)Zjh/J	Tshz1-2A-FlpO	Tshz1-2A-FlpO knock-in mice are designed to have optimized Flp recombinase expression directed to teashirt zinc finger family member 1-expressing cells. These mice may be used to generate conditional mutations for studying the function of TSHZ1 in neuronal differentiation, craniofacial development, and complex cognitive pathways.
038030	B6(Cg)-Meis1^em1Bcca/AkarJ	MEIS1-EGFP	MEIS1-EGFP mice contain a CRISPR/Cas9-generated insertion of a GFP-P2A-HA immediately downstream of the endogenous Meis1 translational start site (TSS). These mice may be useful for tracking Meis1-expressing hematopoietic cells and for exploring MEIS1 function and regulation during normal and leukemic hematopoiesis.
038083	STOCK Is(SYNGAP1-ZBTB9)1Bpro/Mmjax	SYNGAP1	Humanized SYNGAP1^hu knock-in/knock-out mice express human SYNGAP1. RT-qPCR analysis from the mouse cortex indicates that these mice express no detectable mouse Syngap1, but do express an equivalent level of human SYNGAP1. This strain may be useful for studies of intellectual disability (ID) and autism spectrum disorder (ASD).

Strains Added in May 2023

Stock Number	Name	Common Name	Description
038257	STOCK Krt13^{em1(cre/ERT2)Raja}/J	KRT13-CreER	KRT13-CreER knock-in mice express tamoxifen-inducible cre/ERT2 recombinase from the mouse keratin 13 (Krt13) promoter in hillock cells of the tracheal epithelium. This strain has been useful in hillock cell lineage tracing studies with implications for injury repair, squamous metaplasia, and lung cancer.
036633	FVB/NJ-Tg(C9orf72_i3)101Lutzy/J	Tg(C9orf72_3) line 101	Tg(C9orf72_3) line 101 mice have at least 3 tandem copies of the C9orf72_3 transgene, with each copy encoding the human C9orf72 with a hexanucleotide repeat expansion in the intron between the alternatively-spliced non-coding first exons 1a and 1b. Each transgene copy has between 100-750 repeats ([GGGGCC]_100-750). Hemizygous mice exhibit RNA foci and soluble poly(GP) dipeptides in cortex, hippocampus and cerebellum by three months of age. These mice may be useful in applications related to amyotrophic lateral sclerosis and frontotemporal dementia.
037678	B6.129(Cg)-Flt4^tm2.1Ali/OuluJ	Vegfr3	Vegfr3^lx mice possess loxP sites flanking exon 1 of the Flt4 (FMS-like tyrosine kinase 4 also known as Vegfr) gene. These floxed mice are useful for tissue specific deletion of Flt4 when studying the development of the lymphatic, circulatory, and nervous systems.
037878	FVB/N-Tg(KRT14-HPV16E6)5737Plam/J	K14E6	K14E6 transgenic mice carry a human keratin 14 (KRT14) promoter directing expression of the E6 gene from high-risk human papillomavirus type 16 (HPV16) to the basal layer of epithelia. Mice develop hyperproliferation and epidermal hyperplasia. Skin tumors are found in 14% of mice by 15 months of age. This strain may be useful for research involving HPVs. Of note, the K14E7 transgenic strain (expressing HPV16 E7) is distributed as FVB/N-Tg(KRT14-HPV16E7)2304Plam/J (Stock No. 037879).
037879	FVB/N-Tg(KRT14-HPV16E7)2304Plam/J	K14E7	K14E7 transgenic mice carry a human keratin 14 (KRT14) promoter directing expression of the E7 gene from high-risk human papillomavirus type 16 (HPV16) to the basal layer of epithelia. Mice develop epidermal and hyperkeratosis. Skin tumors are found in 3 out of 30 mice by 10.6 months of age. This strain may be useful for research involving HPVs and tumorigenesis. Of note, the K14E6 transgenic strain (expressing HPV16 E6) is distributed as FVB/N-Tg(KRT14-HPV16E6)5737Plam/J (Stock No. 037878).
036653	B6.Cg-Pilra^em1Adiuj/J	Pilra	Pilra^G85R has amino acids at positions 85-88 in the Pilra locus modified from GEFI to RQSF to model the Alzheimer's disease risk variant rs1859788 and humanize the surrounding region.
037960	B6.129-Daxx^em1Glo/J	Daxx	Daxx^fl mice possess loxP sites flanking exon 3 of the Fas death domain-associated protein (Daxx) gene. These mice may be useful in generating conditional mutations for studying apoptosis and regulation of gene transcription.
037890	C57BL/6-Gt(ROSA)26Sor^{tm1(birA)Srgj}/J	Rosa26	Rosa26^TurboID knock-in mice have the widely-expressed CAG promoter controlling the cre-inducible expression of a V5-tagged biotin ligase sequence, TurboID, with a nuclear export signal. Cre-mediated recombination results in the excision of a STOP sequence, enabling cell-type specific expression of TurboID. These mice provide a tool for in vivo biotinylation of proteins allowing characterization of specific proteomes in multiple tissues.
037596	B6.FVB-AU040320^em1Janc/J	Aavr<->, Kiaa0319l<->	Aavr knock-out mice have a frameshift mutation in exon 2 of the AU040320 (also known as Aavr - adeno-associated virus receptor) locus. Mice homozygous for the mutation are resistant to infection with adeno-associated virus (AAV). These mice may be useful in testing virus-based gene therapies.
037554	C57BL/6J-Lyset^em1Janc/J	Lyset KO	Lyset knock-out mice have 184 nucleotides deletion in exon 2 of the Lyset (lysosomal enzyme trafficking factor formerly, Tmem251) locus. Mice homozygous for the mutation exhibit increased lysosomal enzyme activity and morphological alterations in lysosomes. These mice may be useful for studies of severe lysosomal storage disorder mucolipidosis II.
029497	NOD/ShiLtDvs-Tgm2^em1Dvs/DvsJ	NOD.TGM2 KO	NOD.Tgm2 knock-out (KO) mice carry a CRISPR-made deletion of exons 1 and 2 the transglutaminase 2, C polypeptide (Tgm2) gene. These mice may be useful for studying the regulation of cell adhesion and protein aggregation.
037020	C57BL/6J-Nadk2^nmf421/JRwbJ	Nadk2<-/->; nmf421 S330P	Nadk2^nmf421 mice carry a recessive ENU-induced mutation that results in a 65% reduction of NADK2 protein. Mice homozygous for the mutation (Nadk2^-/-) exhibit neuromuscular degeneration, neurodegeneration, and metabolic defects. Homozygotes begin dying by 3-4 months of age.
037821	C57BL/6-Ass1^{tm1c(EUCOMM)Hmgu}/VantJ	ASS1	Ass1^F mice possess loxP sites flanking exon 8 of the argininosuccinate synthetase 1 (Ass1) gene. These mice may be useful for studying arginosuccinate synthetase deficiency in cre-expressing tissues.
028806	STOCK Tg(SNCA)1Xwy/J	BAC-aSyn	These BAC-aSyn mice conditionally express a wild-type human synuclein, alpha (non A4 component of amyloid precursor), SNCA, gene directed by the human SNCA promoter/enhancer regions on the BAC transgene. These mice may be useful for studying SNCA function and the pathogenesis of Parkinson's disease.
028808	FVB(B6)-Tg(SNCA*)BXwy/J	BAC-aSyn120	These BAC-aSyn120 mice express a conditional mutant human synuclein, alpha (non A4 component of amyloid precursor), SNCA, gene directed by the human SNCA promoter/enhancer regions on the BAC transgene that produces a C-terminal truncated protein. These mice may be useful for studying SNCA function and the pathogenesis of Parkinson's disease.
038040	C57BL/6N-Trdc^{tm1(EGFP/HBEGF/luc)Impr}/J	Tcrd-GDL	Tcrd-GDL knock-in mice carry EGFP, the human diphtheria toxin receptor gene (HBEGF), and luciferase inserted into the 3' UTR of the Trdc (T cell receptor delta, constant region) gene. This strain may be used as a reporter for γδ T cell morphology and activity and for the conditional depletion of γδ T cells by injection of diphtheria toxin.
038153	B6.Cg-Tg(Pitx3-IFP,-tdTomato,-Citrine)2Past/Mmjax	Pitx3-ITC	Pitx3-ITC transgenic mice constitutively express an IFP (infrared fluorescent protein) in substantia nigra pars compacta (SNc) midbrain dopaminergic neurons (mDA). In addition, this strain expresses a cre-dependent tdTomato reporter and Flp-dependent Citrine reporter. In this model, conditional reporter expression can be used as an intersectional genetic tool to analyze substantia nigra development and neuron migration in vivo.
038174	C57BL/6-Tg(Slc1a2-RHO/ADRA1,-EYFP)941Hhir/J	Optoα1AR strong TG	Strong TG (line 941) mice express an optically-activatable G protein-coupled adrenergic receptor (Optoα1AR) and the EYFP reporter in astrocytes. Activation of astrocytes through exposure to blue light results in transient elevation of Ca2+. This strain may be useful for assessing astrocyte activation and the effects on neuronal activity and behavior.
037953	B6.Cg-Gt(ROSA)26Sor^{tm4(CAG-cas9/GAG-POL,-mNeonGreen)Tyj}*/J	Rosa26 , LSL PE2	Rosa26^PE2 knock-in mice allow cre-inducible expression of a prime editor (PE2) allele consisting of a human optimized cas9 fused to a MMLV-RT (Moloney murine leukemia virus reverse transcriptase, GAG-POL) and an mNeonGreen reporter. The Rosa26^PE2 model facilitates in vivo prime editing for precise editing of transition and transversion single nucleotide variants (SNVs) and indels in murine tissues and in vitro prime editing in organoids and cell lines derived from various tissues. This strain may be used to accelerate preclinical functional studies of cancer-associated alleles and complex genetic combinations. Of note, a strain carrying the Rosa26^PE2 allele and a floxed Trp53 allele is distributed as B6.Cg-Gt(ROSA)26Sor^{tm4(CAG-cas9/GAG-POL,-mNeonGreen)Tyj} Trp53^tm1Brn*/TyjJ (Stock No. 037954).
037954	B6.Cg-Gt(ROSA)26Sor^{tm4(CAG-cas9/GAG-POL,-mNeonGreen)Tyj} Trp53^tm1Brn*/TyjJ	Rosa26;Trp53	Rosa26^PE2 Trp53^flox knock-in mice allow cre-inducible expression of a prime editor (PE2) allele consisting of a human optimized cas9 fused to a MMLV-RT (Moloney murine leukemia virus reverse transcriptase, GAG-POL) and an mNeonGreen reporter in the context of a cre-mediated Trp53 null allele. The Rosa26^PE2 Trp53^flox model facilitates in vivo prime editing for precise editing of transition and transversion single nucleotide variants (SNVs) and indels in murine tissues and in vitro prime editing in organoids and cell lines derived from various tissues. This strain may be used to accelerate preclinical functional studies of cancer-associated alleles and complex genetic combinations. Of note, a strain carrying only the Rosa26^PE2 allele is distributed as B6.Cg-Gt(ROSA)26Sor^{tm4(CAG-cas9/GAG-POL,-mNeonGreen)Tyj}*/J (Stock No. 037953).
031489	B6;129S-Npr2^tm1.1Laj/Mmjax	Npr2-7E	Npr2-7E mice have 7 glutamate substitutions (7E) in exons 8 and 9 of the Npr2 (natriuretic peptide receptor 2) gene. The 7E mutations prevent NPR2 dephosphorylation and inactivation. Homozygous mice develop longer and stronger bones. These mice may be used for research in signaling in mouse preovulatory follicles and bone growth regulation. Of note, mice conditionally expressing the 7E mutations are available as B6;129-Npr2^tm1.2Laj/Mmjax (MMRRC Stock No. 071310).
038420	C57BL/6J-Lhcgr^em1Laj/Mmjax	HA-LHR	HA-LHR knock-in mice carry a hemagluttin (HA) tag inserted into the N-terminal of the Lhcgr (luteinizing hormone/choriogonadotropin receptor, also called LHR) gene. This strain may be used as a tool for understanding signaling in mouse preovulatory follicles.
038421	C57BL/6J-Npr2^em1Laj/Mmjax	HA-NPR2	HA-NPR2 knock-in mice carry a hemagluttin (HA) tag inserted into the N-terminal of the Npr2 (natriuretic peptide receptor 2) gene. This strain may be used as a tool for understanding signaling in mouse preovulatory follicles and bone growth regulation.
038422	B6;129S-Npr2^tm1.2Laj/Mmjax	Npr2-7E-cKI	Of note, mice constitutively expressing the 7E mutations are available as B6;129-Npr2^tm1.2Laj/Mmjax (MMRRC Stock No. 071312). Conditional Npr2-7E cKI mice have a loxP-flanked EGFP reporter upstream of 7 glutamate substitutions (7E) in exons 8 and 9 of the Npr2 (natriuretic peptide receptor 2) gene. The 7E mutations prevent NPR2 dephosphorylation and inactivation. These floxed mice may be used as a tool for understanding signaling in mouse preovulatory follicles and bone growth regulation.
033122	C57BL/6J-Il2rg^em3Lutzy/J	B6J.Il2rg KO	B6J.Il2rg KO mice have a CRISPR/Cas9-generated deletion of exon 3 of the interleukin 2 receptor, gamma chain locus (Il2rg). These B6J.Il2rg KO mice may be useful for studying NK cell development, innate immunity, T and B cell deficiency.

Previous Strains

Strains Added in July 2023

Strains Added in June 2023

Strains Added in May 2023