First Look Strains

Stock Number	Name	Common Name	Description
034061	C57BL/6J-Stmn2^{em6(STMN2)Lutzy}/Mmjax	Stmn2	Stmn2^em6(STMN2) is a CRISPR/cas9 generated mutant of the stathmin-like 2 (Stmn2) gene carrying 394nt of human STMN2 intron 1 replacing 479 nt of murine Stmn2 intron 1. The effect of this substitution is to introduce a functional exon 2a present in human but absent in mouse into the mouse genome. As exon 2a utilization is predicted to be TDP43 protein dependent, these mice may be useful in pre-clinical studies of Amyotrophic Lateral Sclerosis (ALS).
035602	BXD183/RwwJ		The BXD#/Rww recombinant inbred (RI) strains originate from crosses between C57BL/6J (000664) females and DBA/2J (000671) males and were generated using a strategy of advanced intercrosses (AI). They may be used to study the genetics of behavioral phenotypes (including alcohol and drug addiction, stress, and locomotor activity) and complex or potentially complex physiologic phenotypes (including differences in organ weight and bone mineral density).
035721	C57BL/6J-Stmn2^{em8(STMN2)Lutzy}*/Mmjax	Stmn2	Stmn2^em8(STMN2) is a CRISPR/cas9 generated mutant of the stathmin-like 2 (Stmn2) gene carrying 222 nt of human STMN2* exon 2a. The STMN2 sequence is modified to include the human MS2 stem loop sequence and replace the TDP43 binding element. These mice may be useful in pre-clinical studies of Amyotrophic Lateral Sclerosis (ALS).
036225	C57BL/6J-Pbk^em1Xxh/J	Pbk	Pbk^KI/KI mice carry two CRISPR/Cas9 generated lysine to alanine mutations in exon 5 of the Pbk gene, resulting in an inactive kinase. This strain may be useful when studying the role of PBK in the regulation of high fat diet (HFD)-induced beta cell proliferation, as well as tumor development, cancer growth, apoptosis and inflammation.
037042	STOCK Ascl1^tm2.1Fgu/JejoJ	Ascl1	Ascl1^flox/flox mice have loxP sites flanking the entire coding region of the Ascl1 gene and also contain a Venus fluorescent sequence downstream of the floxed region. This mutant mouse strain may be useful in studying distinct neuronal cell populations, neuronal turnover, and neuronal migration. The donating investigator reports that the level of expression of the Venus reporter gene is below detection via direct fluorescence and immunohisotchemistry.
037071	B6.129-Gt(ROSA)26Sor^{tm1(CAG-cas9,-EGFP)Fezh}* Is(14)2Rdf/CjwuJ	MDRCas9	MDRCas9 double mutant mice contain the 14qC3-MDR allele (Stock No. 017642), containing a loxP- and frt-flanked region on Chr 14 that has been implicated in B cell chronic lymphocytic leukemia (CLL). They also contain a Rosa26-LSL-Cas9 knockin allele (Stock No. 026175) with cre recombinase-dependent expression of CRISPR associated protein 9 (cas9) endonuclease and EGFP directed by a CAG promoter. When bred to MDRCD19 double homozygous mice (Stock No. 037070), these mice have applications in studies related to tumorigenesis and therapeutic management of chronic lymphocytic leukemia.
037205	B6.Cg-Tg(OPTN*E50K,-EGFP)1Mde/J	BAC-hOPTN	BAC-hOPTN^E50K transgenic mice overexpress low, near-physiological levels of human OPTN carrying the glaucoma-associated, autosomal dominant E50K (GAG to AAG) missense mutation fused to IRES-EGFP. Hemizygotes demonstrate age-related loss of retinal ganglion cells (RGCs) and selective visual impairment in contrast sensitivity by 18 months of age.
037206	B6.129-Optn^tm1.1Htse/J	Optn	Exon 1 of the mouse Optn gene is flanked by loxP sites in these Optn^flox conditional mutant mice. Cre recombinase-mediated excision of the floxed region results in a knock-out allele useful in a range of research areas including glaucoma, amyotrophic lateral sclerosis (ALS), and Paget's Disease, as well as Alzheimer's, Parkinson's and Huntington's diseases.
037208	B6.Cg-Sp7^em1(flpo)Oam/J	Osterix-FLPo	Osterix-FLPo knock-in mice are designed to have the endogenous Sp7 (Sp7 transcription factor 7, also known as Osterix) promoter/enhancer regions directing expression of optimized FLP recombinase (FLPo) to osteocytes, osteoblasts, bone marrow adipocytes, and a subset of marrow stromal cells within the bone. These mice are useful for studying bone marrow adipocyte and bone homeostasis. To create BMAd-Cre mice, Osterix-FLPo mice are combined with a FLP-inducible Cre recombinase expressed in adipocytes and bone marrow adipocytes (B6.Cg-Adipoq^em1(cre)Oam/J, Stock No. 037702), this combination creates cre expression directed exclusively to bone marrow adipocytes, but not to osteoblasts or other adipose depots.
037333	B6.Cg-Chodl^em1(cre)Ngai/HzeJ	Chodl-P2A-Cre	Chodl-P2A-Cre knock-in mice are designed to have Cre recombinase expression/activity directed by the endogenous chondrolectin promoter/enhancer sequences. These mice may be used to generate conditional mutations for studying function of Chodl-expressing cells.
037334	B6.Cg-Hpse^em1(cre)Ngai/HzeJ	Hpse-P2A-Cre	Hpse-P2A-Cre knock-in mice are designed to have Cre recombinase expression/activity directed by the endogenous heparanase promoter/enhancer sequences. These mice may be used to generate conditional mutations for studying function of Hpse-expressing cells.
037335	B6.Cg-Parm1^em1(cre)Ngai/HzeJ	Parm1-P2A-Cre	Parm1-P2A-Cre knock-in mice are designed to have Cre recombinase expression/activity directed by the endogenous prostate androgen-regulated mucin-like protein 1 promoter/enhancer sequences. These mice may be used to generate conditional mutations for studying function of Parm1-expressing cells.
037336	B6.Cg-Rxfp1^em1(cre)Ngai/HzeJ	Rxfp1-P2A-Cre	Rxfp1-P2A-Cre knock-in mice are designed to have Cre recombinase expression/activity directed by the endogenous relaxin/insulin-like family peptide receptor 1 promoter/enhancer sequences. These mice may be used to generate conditional mutations for studying function of Rxfp1-expressing cells.
037337	B6.Cg-Slco2a1^em1(cre)Ngai/HzeJ	Slco2a1-P2A-Cre	Slco2a1-P2A-Cre knock-in mice are designed to have Cre recombinase expression/activity directed by the endogenous solute carrier organic anion transporter family member 2a1 promoter/enhancer sequences. These mice may be used to generate conditional mutations for studying function of Slco2a1-expressing cells.
037338	B6.Cg-Cplx3^{em1(flpo)Ngai}*/HzeJ	Cplx3-P2A-FlpO	Cplx3-P2A-FlpO knock-in mice are designed to have optimized Flp recombinase expression directed to complexin 3-expressing cells. These mice may be used to generate conditional mutations for studying the function of this SNARE complex regulator protein in photoreceptor synaptic transmission.
037339	B6.Cg-Cpne4^{em1(flpo)Ngai}*/HzeJ	Cpne4-P2A-FlpO	Cpne4-P2A-FlpO knock-in mice are designed to have optimized Flp recombinase expression directed to copine 4-expressing cells. These mice may be used to generate conditional mutations for studying the function of this calcium-dependent, phospholipid-binding protein.
037340	B6.Cg-Lamp5^{em1(flpo)Ngai}*/HzeJ	Lamp5-P2A-FlpO	Lamp5-P2A-FlpO knock-in mice are designed to have optimized FLP recombinase expression directed to lysosomal associated membrane protein family member 5 (LAMP5)-expressing cells. These mice may be used to generate conditional mutations for studying the function of this lysosomal associated membrane protein and membrane trafficking in synaptic neurotransmission.
037369	STOCK H2^u H2-T18^a Tg(CD2-TcraAc1-9,-TcrbAc1-9)4Kio/WdcJ	Tg4	Tg4 H-2^u mice express rearranged Vα 4/Vβ 8.2 T cell receptor chains generated from T cell hybridoma cDNA raised against the acetylated N-terminal peptide of murine myelin basic protein (MBP), Ac1-9, and directed by human CD2 regulatory elements on the H2^u background. Tg4 mice are highly susceptible to induction of experimental autoimmune encephalomyelitis (EAE). This strain may be useful in studies of immunological tolerance to self-antigen, antigen-specific immunotherapy of autoimmune disease including CNS inflammation and EAE/multiple sclerosis.
037370	STOCK Rag1^tm1Bal H2^u H2-T18^a Tg(CD2-TcraAc1-9,-TcrbAc1-9)4Kio/WdcJ	RTO; Tg4 Rag1-/-	Tg4 Rag-1^-/- (RTO) mice express a B and T cell deficient Rag1 knockout allele combined with a rearranged Vα 4/Vβ 8.2 T cell receptor chains generated from T cell hybridoma cDNA raised against the acetylated N-terminal peptide of murine myelin basic protein (MBP), Ac1-9, and directed by human CD2 regulatory elements on the H2-A^u background. Tg4 Rag-1^-/- mice provide a model for experimental autoimmune encephalomyelitis (EAE) on an immunodeficient background. This strain may be useful in studies of antigen specific immunotherapy in autoimmune disease including CNS inflammation and EAE/multiple sclerosis.
037371	STOCK Ptprc^a Pepc^b H2^u H2-T18^a Tg(CD2-TcraAc1-9,-TcrbAc1-9)4Kio/WdcJ	Tg4 CD45.1	Tg4 CD45.1 mice express the pan leukocyte marker Ptprc^a (CD45.1) and rearranged Vα 4/Vβ 8.2 T cell receptor chains generated from T cell hybridoma cDNA raised against the acetylated N-terminal peptide of murine myelin basic protein (MBP), Ac1-9, and directed by human CD2 regulatory elements on the H2-A^u background. Tg4 CD45.1 mice are highly susceptible to induction of experimental autoimmune encephalomyelitis (EAE). The addition of the CD45.1 marker provides the ability to distinguish CD4+ T cells from this strain and the parental Tg4 CD45.2 strain (Stock No. 037369). This strain may be useful in studies of immunological tolerance to self-antigen, antigen-specific immunotherapy of autoimmune disease including CNS inflammation and EAE/multiple sclerosis.
037372	STOCK Il10^tm1Flv H2^u H2-T18^a Tg(CD2-TcraAc1-9,-TcrbAc1-9)4Kio/WdcJ	Tg4 Tiger; Tg4 IL10/GFP	Tg4 Tiger mice express an Il10 allele with a GFP reporter (Tiger) and rearranged Vα 4/Vβ 8.2 T cell receptor chains generated from T cell hybridoma cDNA raised against the acetylated N-terminal peptide of murine myelin basic protein (MBP), Ac1-9, and directed by human CD2 regulatory elements on the H2-A^u background. Tg4 Tiger mice are highly susceptible to induction of experimental autoimmune encephalomyelitis (EAE). This strain may be useful in studies of IL10 and antigen specific immunotherapy in autoimmune disease including CNS inflammation and EAE/multiple sclerosis.
037450	B6.Cg-Lox^em1Mech/J	Lox	Lox^Mut mice carry a CRISPR/Cas9 generated M292R missense mutation in the copper binding domain of the lysyl oxidase (Lox) gene. These mice may be useful for studying tumor suppression and thoracic aortic aneurysms and dissections (TAAD).
037466	C57BL/6-Tg(Tal1-cre/ERT)42-056Jrg/J	HSC-SCL-CreER	HSC-SCL-CreER^T mice express Cre/ERT fusion gene under the control of the T cell acute lymphocytic leukemia 1 (Tal1) promoter. These mice may be useful for lineage mapping of hematopoietic stem cells.
037467	C57BL/6-Tg(Tal1-cre/ERT)1Jrg/J	endothelial-SCL-Cre-ER	Endothelial-SCL-Cre-ER^T mice express Cre/ERT fusion protein driven by 5'-endothelial-SCL enhancer element. These mice may be useful for lineage mapping of endothelial cells.
037468	STOCK Sftpa1^tm1Haw Tg(SFTPC-SFTPA1*)T2Jflo/Mmjax	hTG SP-A1(6A4)	SP-A KO hTG SP-A1 mice express human SFTPA1 (surfactant associated protein A1 or SP-A1) variant 6A4 under the control of the human SFPTC promoter on a Sftpa1 null background. This strain may be useful for studying lung function, surfactant structure, host defense and inflammation.
037469	STOCK Sftpa1^tm1Haw Tg(SFTPC-SFTPA1*)T1Jflo/Mmjax	SP-A1 (6A<2>)	SP-A KO hTG SP-A1 mice express human SFTPA1 (surfactant associated protein A1 or SP-A1) variant 6A2 under the control of the human SFPTC promoter on a Sftpa1 null background. This strain may be useful for studying lung function, surfactant structure, host defense and inflammation.
037470	STOCK Sftpa1^tm1Haw Tg(SFTPC-SFTPA2*)T10Jflo/Mmjax	SP-A2 (1A<0>)	SP-A KO hTG SP-A2 mice express human SFTPA2 (surfactant associated protein A2 or SP-A2) variant 1A0 under the control of the human SFPTC promoter on a Sftpa1 null background. This strain may be useful for studying lung function, surfactant structure, host defense and inflammation.
037471	STOCK Sftpa1^tm1Haw/JfloMmjax	SP-A KO	SP-A knockout mice have exons 2- 4 of the Sftpa1 (surfactant associated protein A1 or SP-A) gene replaced by a neomycin cassette. Homozygous null mice have an increased susceptibility to IAV (influenza A virus) infection. This strain may be useful for studies of lung surfactant homeostasis and respiratory pathogens.
037474	B6.129S6(SJL)-Wwc1^tm1.2Rlh/J	Kibra conditional KO	KIBRA conditional KO mice have loxP sites flanking exons 4-5 of the Wwc1 gene. These mice may be useful when studying synaptic plasticity, learning, and memory.
037503	CAST/EiJ-Mecp2^em1Grib/J	Mecp2	Mecp2^NLucTom knock-in mice contain a CRISPR/cas9 generated insertion of a NanoLuciferase-P2A-TdTomato (NLucTom) immediately before the stop codon of the Mecp2 gene. These mice may be useful for studying impaired neurodevelopmental maturation associated with human Rett syndrome. They are also useful for studying X chromosome inactivation and reactivation.
037519	FVB.129(B6)-Mertk^tm1Grl/AmenJ	Mer<->	This knock-out mutation of the MER proto-oncogene tyrosine kinase gene (Mertk) exhibits photoreceptor degeneration and abnormal endotoxin response. In combination with mutations in other receptor tyrosine kinases, this Mer^- strain may be useful in studying autoimmunity, germ cell development, homeostatic regulation and apoptosis. Stock No. 037519 is a FVB/N-congenic Mer^- mouse line. Of note, the same Mer^- allele is also available on a mixed genetic background as Stock No. 011122.
037530	B6.Cg-Gt(ROSA)26Sor^{tm1(rtTAM2)Jae} Col1a1^{tm1(tetO-Plk4,-EYFP)Ahol}*/J	Plk4	Plk4^Dox mice may be used to induce overexpression of Plk4 via a tetracycline-controlled transactivator protein system. These mice may be useful for studying the role of Plk4 in centriole biology and centrosome amplification in multiple cancers.
037535	C57BL/6-Pgbd5^tm1.1Aken/J	Pgbd5-floxed	Pgbd5-floxed mice possess loxP sites flanking exon 4 of the piggyBac transposable element derived 5 (Pgbd5) gene. These mice may be useful for studying the role of Pgbd5 in neuronal development and plasticity as well as genomic rearrangements and instability in various cancers.
037536	B6.129S4-Sypl2^tm1Hta/J	MG29	MG29^m1 mice carry a targeted knock-out of the mouse Sypl2 (synaptophysin-like 2; also called MG29) gene, useful in studies of muscle calcium homeostasis and muscle function during aging. Genome-wide association studies (GWAS) have suggested involvement of the gene in Huntington's disease, Alzheimer's disease, kidney disorders, obesity, and severe COVID.
037537	B6.129S4-Srl^tm1Hta/J	Sar<->	Sar^- mice carry a targeted knock-out of the mouse Srl (sarcalumenin) gene, useful in studies of striated (skeletal and cardiac) muscle, muscle fatigue, and Store-Operated Calcium Entry (SOCE).
037538	B6.FVB-Tg(RP11-209M4)AGglo/J	SOST TG , SOST	SOST^wt transgenic mice express human SOST (sclerostin), a negative regulator of bone formation. Homozygous mice develop decreased bone mineral density and fused and missing digits in the forelimbs and hindlimbs. This strain may be useful for studying the physiologic role of SOST and as a model for drug development and mechanism validation.
037540	B6.129(SJL)-Ins2^{tm1.1(cre)Wpiz}/ArvnJ	Ins2-Cre	Ins2-Cre knock-in mice express Cre recombinase under the direction of the Ins2 promoter in β cells of the pancreas.
037558	C57BL/6N-Tg(JAK2*V617F)FF1Rsko/J	JAK2-FF1	JAK2-V617F transgenic mice conditionally express the human JAK2 (Janus kinase 2) gene carrying the pathological V617F substitution. Breeding JAK2-V617F mice to a strain carrying Cre recombinase flips the orientation of the JAK2 cDNA and allows expression of the mutant transgene in offspring. This strain may be useful for studying myeloproliferative disorders, as well as the myeloproliferative neoplasm (MPN) polycythemia vera (PV). Of note, mice carrying the JAK2 exon 12 (N542-E543del) mutation are available as Stock No. 037560).
037578	B6;C3-Oxtr^{em2(icre)Yinn}/J	Oxtr<1xPA-iCre>	Oxtr^1xPA-iCre knock-in mice express a PA-tag and a codon improved cre recombinase (icre) under direction of the endogenous oxytocin receptor (Oxtr) promoter in brain regions. This mouse strain could be useful for manipulation of oxytocin receptor-expressing cells.
037579	B6;C3-Oxtr^{em4(icre/ERT2)Yinn}/J	Oxtr<3xHA-iCreERT2>	Oxtr^{3xHA-iCreERT2} knock-in mice express a 3xHA-tag and icre/ERT2 under direction of the endogenous oxytocin receptor (Oxtr) promoter in brain regions. This mouse strain could be useful for oxytocin receptor visualization and manipulation.
037580	B6;C3-Oxtr^em1Yinn/J	Oxtr<1xPA-tdTom>	Oxtr^1xPA-tdTom knock-in mice express a PA-tag and tdTomato under direction of the endogenous oxytocin receptor (Oxtr) promoter in brain regions. This mouse strain could be useful for oxytocin receptor visualization and manipulation.
037608	BALB/c-Hdc^em1Huhu/J	Hdc, Hdc	Hdc^GCbox mice carry a CRISPR/Cas9-generated deletion of noncoding Hdc (histamine decarboxylase) GC box sequences that possess enhancer activity. Mast cells, basophils, brain cells, and stomach cells from GC box-deficient mice transcribe the Hdc gene much less than similar cells from wildtype mice. Homozygotes fail to develop anaphylaxis following administration of IgE anti-TNP (trinitrophenyl) antibody.
037609	C57BL/6-Ppp1r15a^em1Hato/J	Ppp1r15a uORF	Ppp1r15a uORF mice carry a CRISPR/cas9 generated methionine to isoleucine change change in the start codon of the third upstream open reading frame (uORF3) of the Ppp1r15a gene. This strain may be useful for studying the interplay between kinases and phosphatases involved in the eIF2α axis.
037619	B6.Cg-Rgs2^em1Jgro/J	Rgs2	Rgs2^flox mice possess loxP sites flanking exons 2-4 of the regulator of G-protein signaling 2 (Rgs2) gene. These mice may be useful for studying hypertension and atherosclerosis, as well as neurodegenerative diseases, such as Alzheimer's Disease, and some cancers.
037624	B6(Cg)-Klf9^tm2Sahay/J	Klf9	Klf9^fl conditional mutant mice possess loxP sites flanking exon 1 of the Klf9 (Kruppel-like factor 9) gene and may be useful in generating conditional mutations to study stem cell regulation.
037625	B6.129S(Cg)-Klf9^tm1.1Sahay/J	tetO-Klf9	These teto-Klf9 knock-in mice provide inducible and reversible overexpression of the transcription factor Klf9 (Kruppel-like factor 9) gene regulated by the tetracycline-responsive promoter (tetO). Silencing of KLF9 protein may be regulated with tetracycline or its analog doxycycline (dox) when combined with a strain expressing tetracycline-controlled transcriptional silencer protein (tTS or rtTS). These mice are a Tet tool strain - allowing dox-conditional expression of TRE promoter-driven target genes.
037626	C57BL/6-Gt(ROSA)26Sor^{em1(CAG-Kcnq2/EGFP)Hjch}*/J	Kcnq2-M547V	Kcnq2‐M547V^fl mice carry a floxed‐STOP cassette upstream of a Kcnq2 M547V‐ires‐EGFP cassette placed in the Gt(ROSA)26Sor gene. Upon excision of the floxed‐STOP cassette by Cre recombinase, this line expresses murine Kcnq2 gene containing the epileptic encephalopathy mutation M547V and enhanced green fluorescent protein (EGFP).
037628	STOCK Sftpa1^tm1Haw Tg(SFTPC-SFTPA2*)T1Jflo/Mmjax	hTG SP-A2(1A3)	SP-A KO hTG SP-A2 mice express human SFTPA2 (surfactant associated protein A2 or SP-A2) variant 1A3 under the control of the human SFPTC promoter on a Sftpa1 null background. This strain may be useful for studying lung function, surfactant structure, host defense and inflammation.
037669	C57BL/6J-Tg(SERPINA1)1Mlb/J	PI*M	PiM mice express human SERPINA1* (also known as AAT or α1-antitrypsin), which carries the common M variant (PIM). PIM mouse may be used a coisogenic control for the PIZ transgenic mouse (Stock No. 037670), which carries a glutamic acid to lysine subsitution at residue 342 (E342K). The PIZ variant is associated with alpha-1 antitrypsin (AAT) deficiency.
037670	C57BL/6J-Tg(SERPINA1*E342K)1Mlb/J	Pi*Z	PIZ mice express a mutant human SERPINA1* (also known as AAT or α1-antitrypsin), which carries a glutamic acid to lysine substitution at residue 342 (E342K). The Z allele is associated with Alpha-1 Antitrypsin Deficiency (AATD). PIZ mice exhibit polymerization and accumulation of AAT in the liver as well as the suppression of selected unfolded protein response branches. A coisogenic control carrying the common M variant (PIM) is available: C57BL/6J-Tg(SERPINA1)1Mlb/J Stock No. 037669.
037675	STOCK Ddx25^{tm2.1(DDX25R242H)Mld}*/J	GRTH-KI	GRTH knock-in/knock-out mice have a floxed human DEAD box polypeptide 25 (DDX25) cDNA encoding the human R242H mutation replacing exons 1-5 of the mouse Ddx25 gene. Before exposure to Cre recombinase, these mice express human DDX25 R242H mutation instead of endogenous Ddx25. Upon exposure to Cre recombinase, this is designed to be a knock-out allele. These mice may be useful for studying the role of Ddx25 in spermiogenesis and male fertility.
037677	C57BL/6J-Gt(ROSA)26Sor^em1Naik/J	LoxCode	LoxCode knock-in mice have the LoxCode cassette inserted into the endogenous Gt(ROSA)26Sor locus. The LoxCode cassette is composed of 14 loxP sites in alternating orientation flanking 13 small (8-14nts) code elements. Upon Cre exposure, random recombination between the loxP sites lead to inversions and excisions, resulting in DNA sequence reshuffling with a theoretical DNA diversity of over 30 billion barcodes. This is an inducible, cellular barcoding technology based on the Cre-loxP system that allows for sensitive, high diversity lineage tracing at the cellular, spatial, and tissue level.
037702	B6.Cg-Adipoq^em1(cre)Oam/J	FAC	FAC (FLPo-dependent Adipoq-Cre) knock-in mice express a FLPo-inducible Cre recombinase under the control of the Adipoq (adiponectin, C1Q and collagen domain containing) endogenous promoter. Following FLPo-mediated recombination cre is expressed in adipose tissue. This strain may be used in studies of bone marrow adipocytes and bone homeostasis. To create BMAd-Cre mice, FAC mice are combined with a FLP expressed in osteoblasts and bone marrow adipocytes (B6.Cg-Sp7^em1(flpo)Oam/J, Stock No. 037208), this combination creates cre expression directed exclusively to bone marrow adipocytes, but not to osteoblasts or other adipose depots.
037725	C57BL/6J-Slc25a39^em2Brsy/J	Slc25a39	Slc25a39^fl mice carry a CRISPR/Cas9 generated mutation resulting in loxP sites flanking exons 2-3, including the translation initiation codon, of the Slc25a39 gene. This strain may be useful for studying the regulation of regulated mitochondrial glutathione import.
037727	C57BL/6J-Cst7^em1Aduci/J	Cst7-null	Cst7-null mice carry a CRISPR/Cas9-generated deletion of part of exon 1 and part of intron 1 of the mutant of the cystatin F (leukocystatin) gene. These mice may be useful in pre-clinical studies of Alzheimer's Disease.

Strains Added in October 2022

Stock Number	Name	Common Name	Description
033707	B6C3-Sms^em2Lutzy/J	B6C3F1.Sms	Sms^G56S is a CRISPR/Cas9-generated mutant of the Sms gene harboring the G56S point mutation. This strain may be useful in studies related to X-linked Snyder-Robinson Syndrome mental retardation. Mice on this C57BL/6J-C3H/HeJ mixed background show improved viability over the original C57BL/6J genetic background line (Stock No. 031170).
037511	B6(129S4)-Pou2f3^{tm1.1(cre/ERT2)Imt}/J	Pou2f3-CreERT2-ires-EGFP	Pou2f3-Cre^ERT2-ires-EGFP knock-in mice express a CreER fusion protein and EGFP in cells that endogenously express the POU domain, class 2, transcription factor 3 (encoded by Pou2f3 gene). When these mice are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the floxed sequences in the Pou2f3-expressing cells of the offspring. These mice may be useful for studying proliferation and differentiation of epithelial sensory cells.
037426	C57BL/6-Itgb2^{tm1.1(ITGB2)Kley}/J	hITGB2 KI	Human ITGB2 knock-in/knock-out mice (hITGB2 KI) have a floxed human ITGB2 isoform 1 cDNA sequence replacing exon 2 of the mouse Itgb2 gene. Before exposure to Cre recombinase, this expresses hITGB2 instead of endogenous Itgb2. Upon exposure to Cre recombinase, this is designed to be a knock-out allele. These mice may be a valuable tool for studying immunodeficiency disease such as Leukocyte Adhesion Deficiency (LAD), as well as evaluating β2-integrin function, activation, kinetics, and bio-distribution in vivo.
037333	B6.Cg-Chodl^em1(cre)Ngai/HzeJ	Chodl-P2A-Cre	Chodl-P2A-Cre knock-in mice are designed to have Cre recombinase expression/activity directed by the endogenous chondrolectin promoter/enhancer sequences. These mice may be used to generate conditional mutations for studying function of Chodl-expressing cells.
037334	B6.Cg-Hpse^em1(cre)Ngai/HzeJ	Hpse-P2A-Cre	Hpse-P2A-Cre knock-in mice are designed to have Cre recombinase expression/activity directed by the endogenous heparanase promoter/enhancer sequences. These mice may be used to generate conditional mutations for studying function of Hpse-expressing cells.
037335	B6.Cg-Parm1^em1(cre)Ngai/HzeJ	Parm1-P2A-Cre	Parm1-P2A-Cre knock-in mice are designed to have Cre recombinase expression/activity directed by the endogenous prostate androgen-regulated mucin-like protein 1 promoter/enhancer sequences. These mice may be used to generate conditional mutations for studying function of Parm1-expressing cells.
037336	B6.Cg-Rxfp1^em1(cre)Ngai/HzeJ	Rxfp1-P2A-Cre	Rxfp1-P2A-Cre knock-in mice are designed to have Cre recombinase expression/activity directed by the endogenous relaxin/insulin-like family peptide receptor 1 promoter/enhancer sequences. These mice may be used to generate conditional mutations for studying function of Rxfp1-expressing cells.
037337	B6.Cg-Slco2a1^em1(cre)Ngai/HzeJ	Slco2a1-P2A-Cre	Slco2a1-P2A-Cre knock-in mice are designed to have Cre recombinase expression/activity directed by the endogenous solute carrier organic anion transporter family member 2a1 promoter/enhancer sequences. These mice may be used to generate conditional mutations for studying function of Slco2a1-expressing cells.
037338	B6.Cg-Cplx3^{em1(flpo)Ngai}*/HzeJ	Cplx3-P2A-FlpO	Cplx3-P2A-FlpO knock-in mice are designed to have optimized Flp recombinase expression directed to complexin 3-expressing cells. These mice may be used to generate conditional mutations for studying the function of this SNARE complex regulator protein in photoreceptor synaptic transmission.
037339	B6.Cg-Cpne4^{em1(flpo)Ngai}*/HzeJ	Cpne4-P2A-FlpO	Cpne4-P2A-FlpO knock-in mice are designed to have optimized Flp recombinase expression directed to copine 4-expressing cells. These mice may be used to generate conditional mutations for studying the function of this calcium-dependent, phospholipid-binding protein.
037340	B6.Cg-Lamp5^{em1(flpo)Ngai}*/HzeJ	Lamp5-P2A-FlpO	Lamp5-P2A-FlpO knock-in mice are designed to have optimized FLP recombinase expression directed to lysosomal associated membrane protein family member 5 (LAMP5)-expressing cells. These mice may be used to generate conditional mutations for studying the function of this lysosomal associated membrane protein and membrane trafficking in synaptic neurotransmission.
037458	B6.129(MRL)-Mirc35^tm1Dgk/J	miR-183C	miR-183C^fl conditional mutant mice possess loxP sites flanking microRNAs 182, 96 and 183 and may be useful in generating conditional mutations to study microRNA regulation of immunity and autoimmunity, specifically systemic lupus erythematosus (SLE).
037533	B6.Cg-Tg(Nr5a1-cre)2Klp/HyJ	SF1-Cre	SF1-Cre BAC transgenic mice express Cre recombinase from the mouse Nr5a1 (nuclear receptor subfamily 5, group A, member 1; also called SF-1) promoter in the somatic cells of the gonads, the adrenal cortex, the anterior pituitary, the spleen, and the ventromedial hypothalamic nucleus.
037518	FVB/NJ-Il4ra^em1Amen/J	Il4ra KO	Il4ra knock-out (KO) mice carry a CRISPR/Cas9-made mutation that knocks out exon 4 of the interleukin 4 receptor, alpha (Il4ra) gene. These mice may be useful for studying regulation of type II inflammatory responses, wound healing, cell proliferation and metastasis in various cancers, as well as tumor-associated macrophage activation in multiple cancers.
034149	C57BL/6J-Gldc^b2b2679Clo/LutzyJ	Gldc	This ENU-derived strain (derived from the original Stock No. 022673) carries an S814P mutation in the mouse Gldc (glycine decarboxylase) gene and may be useful in studies of glycine encephalopathy and non-ketotic hyperglycinemia (NKH). Four other ENU-generated mutations on mouse Chromosome 19 detected through exon genome sequencing were bred out of the original strain through marker-assisted backcrosses to C57BL/6J (N10).
037402	C57BL/6J-Epha1^em1Aduci/J	Epha1-P461L	Epha1^P461L is a CRISPR/Cas9-generated mutant of the Eph receptor A1 (Epha1) gene carrying a missense mutation that corresponds to the human SNP rs202178565 found in human EPHA1 and is associated with increased risk of sporadic Alzheimer’s disease (AD). These mice may be useful in pre-clinical studies of AD.
037496	C57BL/6J-Clu^{em1(CLU)Aduci}*/J	Clu-h2kbKI	Clu-h2kbKI mice contain a CRISPR/Cas9-generated replacement of part of the mouse clusterin (Clu) gene with an approximately 2kb region of human DNA sequence, including a human late-onset Alzheimer's disease (LOAD) risk allele (SNP rs2279590). These mice may be useful in pre-clinical studies of AD.
037497	C57BL/6-Trem2^{em2(TREM2R47H)Aduci}*/J	hTREM2-R47H_KI	hTREM2R47H_KI* mice contain human TREM sequence, with a R47H mutation, replacing the corresponding mouse sequence. These mice may be useful in pre-clinical studies of Alzheimer's disease (AD).
035733	B6(129S4)-Nras^tm1.1Kshn/J	Nras	Nras^G12D,C181S mice carry the point mutations G12D in exon 1 and C181S in exon 5 of the neuroblastoma ras oncogene (Nras) gene, expression of which is blocked by the presence of a loxP-flanked STOP cassette. When bred to a strain expressing Cre recombinase under control of various tissue specific promoters, Cre recombination deletes the LSL cassette and allows the expression of the mutant NRAS oncogenic protein. This strain may be useful in studying cancer and development, such as palmitoylation cycle in NRAS mutant blood cancers and myeloid transformation.
035732	B6.129S6-Kras^tm2Kshn/J	Kras	Kras^LSL-P34R mice carry a point mutation (P34R) in exon 1 of the Kirsten rat sarcoma viral oncogene homolog (Kras) gene, expression of which is blocked by the presence of a loxP-flanked STOP cassette. When bred to a strain expressing Cre recombinase under control of various tissue specific promoters, Cre recombination deletes the LSL cassette and allows the expression of the mutant KRAS oncogenic protein. This strain may be useful in studying RASopathies, cancer and development.
035731	B6.129S6-Kras^tm1Kshn/J	Kras	Kras^LSL-T58I mice carry a point mutation (T58I) in exon 2 of the Kirsten rat sarcoma viral oncogene homolog (Kras) gene, expression of which is blocked by the presence of a loxP-flanked STOP cassette. When bred to a strain expressing Cre recombinase under control of various tissue specific promoters, Cre recombination deletes the LSL cassette and allows the expression of the mutant KRAS oncogenic protein. This strain may be useful in studying RASopathies, cancer and development.
036796	B6.Cg-Actn2^tm1Begg/WtpJ	Actn2	Actn2^F/F mice have loxP sites flanking exons 2-4 of the Actn2 gene. This mutant mouse strain may be useful when studying the cell-autonomous roles of sarcomeres in postnatal cardiomyocyte maturation.
037604	STOCK Gt(ROSA)26Sor^{tm1.1(CAG-HL9-MGV70A,-mBFP,-SNCA,-mTFP1,-SNCAA30P,-mKO2,-SNCAA53T)Rali}* Tg(Vil1-cre/ERT2)23Syr/J	SNCAbow;Vil‐Cre	These SNCAbow;Vil-Cre^ERT2 mice contain α‐synuclein-Crainbow (SNCAbow) reporter and a transgene with tamoxifen-inducible Cre recombinase expression in intestinal epithelium (Vil-Cre^ERT2), making this strain useful for studying the role of α‐synuclein expression in gut mucosal cells in early stages of Parkinson's and related neurodegenerative diseases. α‐synuclein-Crainbow (SNCAbow) has a CAG promoter followed by three pairs of orthogonal lox sites (LoxN, Lox2272, and LoxP), and the SNCAbow construct, all targeted into the Gt(ROSA)26Sor locus. SNCAbow contains four fluorescent proteins, three of which are associated with three human α-synuclein protein sequences (SNCA^WT, SNCA^A30P, or SNCA^A53T), and functions as a Cre recombinase-inducible reporter of either a chemically inducible near‐infrared fluorogen‐activating peptide (FAPMars1) or one of the three nuclear localized fluorescent proteins. This reporter allele is available on its own as Stock No. 037603. The Vil-Cre^ERT2 transgene is available on its own as Stock No. 020282.
037523	C57BL/6J-Arid5a^em1Gaff/J	Arid5a<->	Arid5a^-/- knock-out mice carry a CRISPR/Cas9 generated deletion of a 3,359 bp region of the Arid5a gene containing exons 3-6 and part of exon 7. These mice are useful when studying signaling pathways critical in host defense and those required in autoimmunity.
037603	129S;B6-Gt(ROSA)26Sor^{tm1.1(CAG-HL9-MGV70A,-mBFP,-SNCA,-mTFP1,-SNCAA30P,-mKO2,-SNCAA53T)Rali}*/J	SNCAbow	These α‐synuclein-Crainbow (SNCAbow) mice have a CAG promoter followed by three pairs of orthogonal lox sites (LoxN, Lox2272, and LoxP), and the SNCAbow construct, all targeted into the Gt(ROSA)26Sor locus. SNCAbow contains four fluorescent proteins, three of which are associated with three human α-synuclein protein sequences (SNCA^WT, SNCA^A30P, or SNCA^A53T), and functions as a Cre recombinase-inducible reporter of either a chemically inducible near‐infrared fluorogen‐activating peptide (FAPMars1) or one of the three nuclear localized fluorescent proteins. This strain may be useful for studying the role of α‐synuclein expression Parkinson's and related neurodegenerative diseases. Of note, this reporter allele is also available with a transgene driving tamoxifen-inducible Cre recombinase expression to intestinal epithelium - see SNCAbow;Vil-Cre^ERT2 (Stock No. 037604).
037350	C57BL/6N-Chd8^em1Fezh/J	Chd8<->	Chd8^+/- knock-out mice carry a CRISPR/Cas9 generated deletion of a 7-nucleotide region in exon 1 of the Chd8 gene, resulting in a frameshift mutation. These mice are useful when studying the roles of chromatin modification and striatal dysfunction on the molecular pathology of autism spectrum disorder (ASD).
037548	B6.129S4-Xist^tm2Jae/GribJ	Xist<2lox>	Xist^2lox/2lox floxed mice have loxP sites flanking exons 1-3, as well as 5 kb of the proximal promoter, of the X-linked Xist gene. These mice may be useful when studying X chromosome inactivation and reactivation.
037530	B6.Cg-Gt(ROSA)26Sor^{tm1(rtTAM2)Jae} Col1a1^{tm1(tetO-Plk4,-EYFP)Ahol}*/J	Plk4	Plk4^Dox mice may be used to induce overexpression of Plk4 via a tetracycline-controlled transactivator protein system. These mice may be useful for studying the role of Plk4 in centriole biology and centrosome amplification in multiple cancers.

Strains Added in August 2022

Stock Number	Name	Common Name	Description
037255	C57BL/6-Mecp2^em1Gfng/J	Mecp2 huExon4*R270X	B6-MeCP2-huExon4R270X knock-in mice express a humanized exon 4 and the R270X (arginine to stop) amino acid substitution in the Mecp2* (methyl CpG binding protein 2) locus. The R270X mutation is associated with Rett Syndrome (RTT). Heterozygous females and hemizygous males develop RTT-like symptoms including reduced brain weight, decreased locomotor activity, and breathing problems. These mice be useful in studies of Rett Syndrome and gene therapy testing. Of note, as a control, we recommend the B6-MeCP2-huExon4 model (Stock No. 037256), which carries the same humanized exon 4 region but lack the RTT-associated mutations.
037256	C57BL/6-Mecp2^em2Gfng/J	Mecp2 huExon4	B6-MeCP2-huExon4 knock-in mice express humanized exon 4 inserted into the mouse Mecp2 (methyl CpG binding protein 2) locus. This strain serves as a control for B6-MeCP2-huExon4*R270X (Stock No. 037255). These mice be useful in studies of Rett Syndrome and gene therapy testing.
037209	B6.129(FVB)-Gabra1^tm1.1Uru/J	α1(H101R)	α1(H101R) knock-in mice were generated by incorporating a point mutation in exon 4 of the Gabra1 gene, which encodes a single amino acid substitution (Histidine-101 to Arginine). These mice may be useful for studying physiologic and therapeutic properties of GABA_A α-subunits in such diseases as epilepsy or substance abuse disorders.
037210	B6.129(Cg)-Gabra2^tm1.1Uru/J	α2(H101R)	α2(H101R) knock-in mice were generated by incorporating a point mutation in exon 4 of the Gabra2 gene, which encodes a single amino acid substitution (Histidine-101 to Arginine). These mice may be useful for studying physiologic and therapeutic properties of GABA_A α-subunits in such diseases as epilepsy or substance abuse disorders.
037211	B6.129(Cg)-Gabra3^tm1.1Uru/J	α3(H126R)	α3(H126R) knock-in mice were generated by incorporating a point mutation in exon 4 of the Gabra3 gene, which encodes a single amino acid substitution (Histidine-126 to Arginine). These mice may be useful for studying physiologic and therapeutic properties of GABA_A α-subunits in such diseases as epilepsy or substance abuse disorders.
037212	STOCK Gabra5^tm1.1Uru/J	α5(H105R)	α5(H105R) knock-in mice were generated by incorporating a point mutation in exon 4 of the Gabra5 gene, which encodes a single amino acid substitution (Histidine-105 to Arginine). These mice may be useful for studying physiologic and therapeutic properties of GABA_A α-subunits in such diseases as epilepsy or substance abuse disorders.
037310	STOCK Lmna^tm1.1Hjw/J	Lmna	Lmna^L648R knock-in mice express an L648R (leucine to arginine) amino acid substitution in the Lmna (lamin A) locus. Mice homozygous for the mutation exhibit cranial, mandibular, and dental defects, as well as a slightly shortened lifespan. This strain may be useful for studies of aging.
037331	B6;129S1-Aptx^tm1Pmc/PmthJ	Aptx<->	Aptx^-/- mice lack the two exons encoding the HIT domain of the aprataxin gene. This strain may be useful for studying the role of oxidative stress and DNA break repair in the onset of neurological conditions such as Ataxia Telangiectasia (A-T) and Ataxia oculomotor apraxia 1 (AOA1).
037040	C57BL/6J-Pou2f3^em1Cbwi/J	Pou2f3<->	Pou2f3^-/- knock-out mice have 8 base pairs deleted from exon 4 of Pou2f3 (POU domain, class 2, transcription factor 3) locus. Mice homozygous for the mutation lack the ability to taste, have impaired ability to sense helminth and parasite infection, and are resistant to persistent murine norovirus. These mice be useful in studies of norovirus and parasite pathogenicity, tuft cells, and taste.
031922	C57BL/6J-Fxn^em10Lutzy/J	Fxn	Fxn^I151F is a CRISPR/Cas9 generated mutant of the frataxin (Fxn) gene carrying the I151F knock-in mutation, corresponding to the human I154F point mutation. These mice may be useful in pre-clinical studies of Friedreich's Ataxia. Our preclinical efficacy testing services offer scientific expertise and an array of target-based and phenotype-based outcome measures, both in vivo and at endpoint, for flexible study designs and assay development in mouse models of Friedreich's Ataxia. See our full service platform.
037192	B6;FVB-Tg(Thy1-CAST)12Rnix/J	hCAST Tg, Thy-1-hCAST line 12	hCAST Tg mice express the human CAST (calpastatin) gene under the control of the neuron specific Thy1.1 promoter. hCAST is highly expressed in neuronal tissues including brain, optic nerve, spinal cord, sciatic nerve, and motor neurons (but not astrocytes). This strain may be useful for studying the function of calpain in neurodegenerative disease including ALS, Alzheimer’s, Huntington’s, FTDP, and Parkinson’s.
026883	B6.129S6(SJL)-Klb^tm1.1Sakl/J	Klb	Klb^fl mice have loxP sites flanking exon 1 of the Klb gene. These mice are useful when studying nutrient regulation, metabolic homeostasis, and water consumption.
037100	C57BL/6-Khdrbs1^tm1Gqin/J	Sam68	Sam68^f/f floxed mice have loxP sites flanking exons 5-8 of the Khdrbs1 gene. This mutant mouse strain may be useful in studies of hepatic gluconeogenesis and type 2 diabetes.
037260	B6(129S4)-Ncf2^{tm1c(EUCOMM)Wtsi}/DinJ	Ncf2	Ncf2^fl/fl floxed mice have loxP sites flanking exon 3 of the neutrophil cytosolic factor 2 gene. These mice may be suitable for use in studies related to chronic granulomatous disease and reactive oxygen species (ROS) and their roles in host defense and immunoregulation.
037528	B6.129S6(Cg)-Foxo3^tm1.2Rdp/JussJ	FoxO3	FoxO3^L/L floxed mice may be useful for studying the regulatory role of the FOXO3 transcription factor on cell survival and tumor suppression. Stock No. 037528 is a C57BL/6J-congenic FoxO3^L/L floxed mouse line. Of note, the same FoxO3^L/L floxed allele is also available on a mixed genetic background as Stock No. 024668.
037499	B6.129S6(Cg)-Foxo1^tm1Rdp/JussJ	FoxO1	FoxO1^L floxed mice may be useful for studying the regulatory role of the FOXO1 transcription factor on insulin signaling, glucose production, diabetic cardiomyopathy and tumor suppression. Stock No. 037499 is a C57BL/6J-congenic FoxO1^L floxed mouse line. Of note, the same FoxO1^L floxed allele is also available on a mixed genetic background as Stock No. 024756.
036670	B6.Cg-Polg^tm1Prol Prkn^tm1Shn/J	PolgA; Parkin-	The double mutant Stock No. 036670 was created by breeding PolgA^D257Aneo males from Stock No. 017341 (B6.129S7(Cg)-Polg^tm1Prol/J) to Parkin knock-out females from Stock No. 006582 (B6.129S4-Prkn^tm1Shn/J). The Polg^D257A mitochondrial mutator mice have a D257A mutation in the N-terminal "proofreading" exonuclease domain of the DNA polymerase γ gene (Polg), rendering the expressed mutant protein devoid of polymerase proofreading function in mitochondria. Homozygous Polg^D257A mice develop a premature aging phenotype. Prkn (parkin RBR E3 ubiquitin protein ligase) knock-out mice develop increased extracellular dopamine concentration in the striatum, dysfunctional nigrostriatal pathways, and dysfunctional mitochondria. Double mutant mice gain less weight than either controls or the Polg^D257A homozygotes. This strain maybe useful for studies of mitochondrial dysfunction.
037396	STOCK Rbbp8^tm2Rjbr/J	Ctip	The Ctip^T855A allele has a single amino acid substitution (Threonine-855 to Alanine) in the mouse Retinoblastoma Binding Protein 8 (Rbbp8, also called Ctip) gene. This point mutation removes an ATM/ATR phosphorylation site that regulates the DNA resection activity of the Ctip/MRN complex. These mice may be useful for studying DNA resection and homology-directed repair after DNA damage, as well as cancer biology.
031132	B6.Cg-Tnni3k^tm1.1Tfo/HsvJ	Tnni3k knock-out	Tnni3k KO mice lack exons 1-2 of the TNNI3 interacting kinase (Tnni3k) gene, making them useful when studying oxidative stress, injury, and adverse remodeling in the ischemic heart.
036767	B6.FVB-Mir486^em1Mtm/MsalxJ	miR-486 KO	miR-486 knock-out mice have 85 base pairs deleted from the Mir486 (microRNA 486) locus. Mice homozygous for the mutation exhibit cardiomyopathy and skeletal muscle weakness and slightly decreased fertility in females. These mice be useful in studies of dystrophic skeletal muscles and cardiac muscle pathologies as well as Ago2-dependent RNA silencing in the hematopoietic system. Please note: Mir486 KO mice on the FVB/N background are available as FVB/N-Mir486^em1Mtm/MsalxJ Stock No. 036901.
037120	B6.Cg-Ncl^em1Mfz/J	GAR<->	GAR^+/- mice carry a CRISPR/Cas9 generated deletion of the glycine arginine rich (GAR) domain of nucleolin gene. The GAR domain drives subcellular localization nucleolin, making these mice useful when studying the localization and growth of neurons associated with some neurodegenerative diseases.
037549	B6;SJL-Plk4^em2Ahol/J	Plk4	Plk4^f mice possess loxP sites flanking exon 5 of the Plk4 (Polo-like kinase 4) gene. These mice may be useful for studying the role of Plk4 in centriole biology and centrosome loss in multiple disorders.
037526	C57BL/6N-Opn3^em1Eoan/J	OPN3-mCh	Opn3-mCh knock-in mice carry a CRISPR/Cas9 generated knock-in that expresses a fusion protein OPN3-mCherry under the endogenous opsin 3 (Opn3) promoter. These mice may be useful for studying opsin 3 localization and function at the subcellular level in the brain and peripheral tissues.
037529	C57BL/6J-Ankrd26^em1Ahol/J	Ankrd26<->	Ankrd26^- mice carry a CRISPR/cas9 generated deletion of exons 23-30 of the ankyrin repeat domain 26 (Ankrd26) gene. These mice may be useful for studying the role of Ankrd26 in centriole signaling related to centrosome amplification and polyploidy. This is important in the context of cancer pathogenesis and chronic liver disease.
037482	B6;SJL-Plk4^em1Ahol/J	Plk4<Δu>	Plk4^Δu mice carry a CRISPR/Cas9-made mutation that knocks out start codons of the Plk4 upstream open reading frames uORF1 and uORF2. These mice may be useful for studying the role of Plk4 in centriole biology, regulation of gametogenesis and centrosome amplification in multiple cancers.
037560	C57BL/6N-Tg(JAK2*)N1Rsko/J	JAK2-N1; JAK2-N542-E543del	JAK2-N542-E543del transgenic mice conditionally express the human JAK2 (Janus kinase 2) gene carrying a deletion of codons 542 and 543 (AATGAA) in exon 12. Breeding JAK2-N542-E543del mice to a strain carrying Cre recombinase flips the orientation of the JAK2 cDNA and allows expression of the mutant transgene in offspring. This strain may be useful for studying the myeloproliferative neoplasm (MPN) polycythemia vera (PV). Of note, mice carrying the JAK2 V617F mutation are available as Stock No. 037558).
037266	C57BL/6-Eprs^em1Pyao/Mmjax	Eprs gKO	Eprs gKO mice have premature stop codons inserted in exon 3 of the Eprs (glutamyl-prolyl-tRNA synthetase) locus. In the homozygous state, the mutation is embryonic lethal. Heterozygotes are resistant to isoproterenol, myocardial infarction (MI) and transverse aortic constriction (TAC)-induced cardiac fibrosis. These mice be useful in studies of the regulation of profibrotic genes during cardiac fibrosis.
036737	STOCK Brca1^tm1Thl/J	Brca1, Brca1	Exon 2 of the mouse Brca1 gene, encoding the initiator methionine and part of the RING domain, is flanked by loxP sites in these Brca1^flex2 (Brca1^co) mice. Excision of the floxed region results in the expression of a defective, amino-terminally truncated "RINGless" polypeptide that initiates at methionine residue M90 or M99 (Li, 2016). Targeted inactivation in mammary epithelial cells in females results in the development of mammary tumors that resemble the basal-like triple-negative breast tumors of human BRCA1 mutation carriers (Shakya, 2008).
036649	STOCK Rbbp8^tm1.1Rjbr/J	Ctip	Exon 2 of the mouse Rbbp8 (retinoblastoma binding protein 8, endonuclease; also called CtIP) gene is flanked by loxP sites in these Ctip^co conditional mutant mice (Reczek, 2016). Cre recombinase-mediated excision of the floxed region results in a defective allele that encodes an amino‐terminally truncated polypeptide, initiated at methionines M120 and/or M141 (Wang, 2020). Widespread, homozygous knock-out of the gene is embryonic lethal (Reczek, 2016), while progenitor B cell-specific inactivation of the gene (created through crosses with Cd79a^tm1(cre)Reth (Stock No. 020505) mice) impairs early B cell development (Liu, 2019).
030368	FVB-Tg(Myh6/tetO-Bex1)12.2Jmol/J	Bex1 Tg	These animals conditionally over-express full-length mouse Bex1 (brain expressed X-linked 1) cDNA from the cardiac-specific Myh6/tetO promoter. When bred with transgenic mice expressing a tetracycline transactivator or reverse transactivator protein, cardiac-specific expression can be controlled by withdrawal or administration of a tetracycline analog.
031094	B6(Cg)-Nrxn2^tm7.1Sud/J	NRXN2a/b flox/flox	Exon 18 of the mouse Nrnx2 gene is flanked by loxP sites. Cre-mediated excision of the floxed region results in a knock-out allele.
031598	B6.129S6(C)-Smarca4^tm2.1Grc/J	BRG1(N1507A)-Dendra2	These knock-in mice express a Dendra2 photoswitchable reporter from the mouse Smarca4 (Brg1) promoter. An N1507A mutation in the Smarca4 bromodomain is useful in studies of histone/DNA binding and chromatin remodeling. Neither the N1507A or Dendra2 tag disrupt normal expression of the targeted gene. The N1507A mutation is believed to be the cause of homozygous embryonic lethality.
032608	C57BL/6-Atrx^tm1Hzo/J	Atrx-GFP-Neo	These Atrx-GFP-Neo mice express reduced levels of GFP-tagged protein from the X-linked Atrx (ATRX, chromatin remodeler) gene. Excision of a loxP-flanked neomycin cassette restores normal expression levels of the fluorescently-labeled ATRX protein. This strain has applications in Rett Syndrome and Alpha-Thalassemia studies.
032855	C57BL/6J-Pnldc1^em1Pdz/J	Pnldc1<->	Pnldc1^- mice carry a CRISPR/Cas9-derived knock-out of the mouse Pnldc1 gene, useful in studies of piRNA biogenesis.
035186	B6.Cg-Htra3^tm1Sud Tyr^c-2J/J	5-HT3A cKO	In these 5-HT3A cKO mice, exon 2 of the mouse Htr3a (5-hydroxytryptamine (serotonin) receptor 3A; also called 5-HT3A) gene was replaced by a cassette consisting of two versions of exon 2: FLP-mediated recombination converts it randomly into HA-mVenus tagged exon 2 or FLAG tagged exon 2. Cre-mediated recombination is anticipated to result in deletion of exon 2 and a knock-out of the gene (not yet confirmed - April, 2020). This is a tool mouse line that will be useful for the community for identification of 5HT3A-receptor positive interneurons.
036591	C57BL/6J-Fcrl2^em1(cre)Gfng/J	Fcrls-2A-Cre	Fcrls-2A-Cre knock-in mice have the endogenous Fcrl2 (formerly Fcrls) promoter/enhancer sequences directing expression of Cre recombinase. These mice are a Cre-lox tool allowing Cre recombination in Fcrl2-expressing cells/tissues (primarily microglia), and may be useful in studying neural development and neurodegenerative diseases.
037118	B6.129S-Podn^tm1Pklt/J	podocan<->	Podocan^-/- mice have a neo cassette replacing exons 4-8 of the Podn gene, abolishing endogenous gene expression. These mice may be useful for studying cellular responses to injury.
037551	NOD.Cg-Tg(Pdx1-cre/Esr1*)1Mga/MirmJ	NOD.pdx1 CreER	This tamoxifen-inducible pdx1^PB CreER^TM strain on the diabetes susceptible NOD background may be used to create inducible pancreatic cell knockouts when crossed with strains carrying floxed alleles.
037552	NOD.Cg-Alox15^tm1.1Nadl/MirmJ	NOD-12/15-LO, NOD:Alox15	NOD:Alox15^loxP mice possess loxP sites flanking exons 2-5 of the arachidonate 15-lipoxygenase (Alox15) gene on the diabetes susceptible NOD/ShiLtJ background. These floxed mice are useful for tissue specific deletion of Alox15 when studying obesity and diabetes. 12/15-LO^loxP/loxP is available on the C57BL/6 background as Stock No. 031835.
037514	C57BL/6J-Appbp2^em1Kajim/J	Appbp2 flox	Appbp2 flox mice, in which exon 2 of the mouse Appbp2 (amyloid beta precursor protein (cytoplasmic tail) binding protein 2) gene is flanked by loxP sites, may be useful for studying the role of the gene in the regulation of thermogenesis, obesity, and diabetes. Cre recombinase-mediated excision of the floxed region results in a knock-out allele.
037267	B6J.Cg-Mettl5^em1.1Snac/J	Mettl5 floxed	Exon 2 of the mouse Mettl5 (methyltransferase like 5) gene is flanked by loxP sites. Cre recombinase-mediated excision of the exon results in a knock-out allele useful in studies of methylation, mRNA translation, and metabolic dysregulation. Of note, the donating investigator has also made a derived Mettl5 knock-out mouse line available (Stock No. 037268).
037268	STOCK Mettl5^em1.2Snac/J	Mettl5<->	Exon 2 of the mouse Mettl5 (methyltransferase like 5) gene has been excised to create this knock-out allele that is useful in studies of methylation, mRNA translation, and metabolic dysregulation. Of note, the donating investigator has also made a Mettl5 floxed mouse line available (Stock No. 037267).
037393	B6.Cg-Gt(ROSA)26Sor^{em1(CAG-Cd55)Hgr}*/J	DAF-TM	DAF-TM mice have a CRISPR/Cas9 generated insertion of a floxed STOP cassette and a mutated Cd55 gene inserted into the endogenous Gt(ROSA)26Sor locus. Specifically, the DAF-TM sequence contains the complement regulatory domain of the CD55 molecule, decay accelerating factor for complement (Cd55) gene, and the signal sequence for glycophosphatidylinositol-(GPI)-anchor addition of Cd55 replaced with that of the transmembrane helix domain of human tissue factor. When crossed with a Cre recombinase expressing strain, removal of the floxed STOP cassette will result in DAF-TM expression in Cre expressing tissues. These mice may be useful for studying complement activation and signaling cascades.
037569	C57BL/6J-Henmt1^em1Pdz/J	Henmt1	Henmt1^em1 mutant mice carry a CRISPR/cas9-generated mutation of the mouse Henmt1 (HEN1 methyltransferase homolog 1 (Arabidopsis)) gene. Transcription of the targeted gene is unperturbed, but the mutation in exon 4 results in a loss of catalytic activity and reduced expression of the protein. These mice are useful for studies of spermatogenesis, and piRNA biogenesis.

First Look Strains

Featured "First Look" Platforms:

Oxtr^1xPAiCre

HSC-SCL-CreERT

BAC-hOPTN^E50K

LoxCode

Osterix-FLPo

iPSC

Strains Added in October 2022

Strains Added in August 2022

First Look Strains

Featured "First Look" Platforms:

Oxtr1xPAiCre

HSC-SCL-CreERT

BAC-hOPTNE50K

LoxCode

Osterix-FLPo

iPSC

Strains Added in October 2022

Strains Added in August 2022

Oxtr^1xPAiCre

BAC-hOPTN^E50K