Please contact the event organizer for more information
Sep 22 - 27
2019
This is a unique didactic/hands-on workshop that provides a comprehensive overview of respiratory biology and, in particular, the use of mice as model systems of human lung diseases. The objectives of the workshop include reviews of basic lung biology, structure-function issues surrounding respiratory mechanics, immune responses linked with the pulmonary compartments as well as the lung microenvironment, and the techniques/strategies for reproducible measurements of lung function in mice. Laboratory-based exercises will provide participants with hands-on experience measuring airway hyper-responsiveness (AHR), lung volumes and pressure-volume (PV) curves, lung morphometry, sampling techniques and mouse lung histopathology. In addition, the course will review basic mouse genetics, housing and breeding strategies, and survival surgical techniques. The course faculty is comprised of prominent members of the research community who are supported by a core group of research scientists providing insights into cutting edge technologies and experimental strategies.
Part of this workshop includes pathology sessions. If you have any FIXED slides you would like to have analyzed by the workshop pathologist, please bring them with you. Additionally, if you have any interesting FIXED slides you would like to share with the group, please bring these as well. IMPORTANT NOTE: DO NOT BRING FRESH TISSUE TO THE JACKSON LABORATORY!
During this workshop, participants have the opportunity to present their work (or proposed work) and receive constructive and encouraging feedback from course faculty. While not a requirement, participants are strongly encouraged to take advantage of this offer and bring a 10 minute PowerPoint presentation. Presentations have the added benefit of promoting dialogue and potential collaborative opportunities among fellow participants and faculty.
Participants completing this workshop will have a working knowledge of basic pulmonary biology and the pathophysiology/histopathology accompanying lung diseases. More importantly, participants will gain the needed exposure to laboratory-based techniques for quantifying pulmonary structure, immunobiology, and lung function – all key endpoint metrics needed to utilize mice as model systems of human respiratory diseases.
8:30 PM |
Sunday, September 22nd 4:30pm Registration and reception 6:00pm Dinner 7:30pm Orientation and Introductions Wayne Mitzner, Ph.D., John Hopkins Bloomberg School of Public Health Michael Borchers, Ph.D., University of Cincinnati Jennifer Corrigan, M.S., The Jackson Laboratory |
12:00 PM |
Monday, September 23rd 8:00am Welcome Wayne Mitzner, Ph.D., John Hopkins Bloomberg School of Public Health Mouse Models of Lung Disease Wayne Mitzner, Ph.D., John Hopkins Bloomberg School of Public Health
9:30am Normal Mouse Cytology and the Role of Immune Cells in Mouse Models of Lung Disease Michael Borchers, Ph.D., University of Cincinnati
10:30am Introduction to Mouse Ventilation Systems Lennart K.A. Lundblad, Ph.D., Meakins-Christie Labs, McGill University
11:30am Lunch
12:30pm Mouse Husbandry, Breeding and Genetic Quality Control TBA
1:45pm Hands-on Mouse Techniques: Handling, Restraint & IP Injection Kristen Cough, B.S., The Jackson Laboratory
3:15pm Sampling Techniques: Cardiac Puncture, Mandibular Blood Collection & Bone Marrow Collection Michael Borchers, Ph.D., University of Cincinnati Jennifer Corrigan, M.S., The Jackson Laboratory Kristen Cough, B.S., The Jackson Laboratory Jeffrey Loube, B.S., John Hopkins Bloomberg School of Public Health
4:30pm Blood Smears Michael Borchers, Ph.D., University of Cincinnati
6:00pm Dinner
7:00pm Participant Presentations (Bring a 10 min PowerPoint presentation of your work.) |
12:00 PM |
Tuesday, September 24th 8:00am Foundations of Lung Function and Assessment with Forced Oscillation Lennart K.A. Lundblad, Ph.D., Meakins-Christie Labs, McGill University
9:00am Lung Volumes, Pressure-Volume Curves, and Lung Diffusing Capacity Wayne Mitzner, Ph.D., John Hopkins Bloomberg School of Public Health
10:00am Mouse Lung Intubation Wayne Mitzner, Ph.D., John Hopkins Bloomberg School of Public Health
10:15 am Introduction to the Flexivent Annette Robichaud, Ph.D., SCIREQ Scientific Respiratory Equipment Inc.
11:30am Tracheostomy Jeffrey Loube, B.S., John Hopkins Bloomberg School of Public Health Wayne Mitzner, Ph.D., John Hopkins Bloomberg School of Public Health
12:30pm Lunch
1:30pm Concurrent Rotating Labs
Annette Robichaud, Ph.D., SCIREQ Scientific Respiratory Equipment Inc. Lennart K.A. Lundblad, Ph.D., Meakins-Christie Labs, McGill University
Michael Borchers, Ph.D., Univ of Cincinnati
3:30pm Rotate Concurrent Labs
6:00pm Dinner 7:00pm Participant Presentations (Bring a 10 min PowerPoint presentation of your work.) |
12:00 PM |
Wednesday, September 25th 8:00am Introduction to the Light Microscope and Optimizing Image Acquisition Douglas Taatjes, Ph.D., The University of Vermont
9:00am Pathology of Mouse Models of Lung Disease Cory Brayton, D.V.M., ACVP, John Hopkins University School of Medicine
10:15am Harvest & Fixation Techniques Wayne Mitzner, Ph.D., John Hopkins Bloomberg School of Public Health
10:45am Introduction to Morphometry/Stereology and Sampling as Applied to the Lung Christian Mühlfeld, Ph.D., Hannover Medical School
11:45am Lunch
1:00pm Concurrent Rotating Labs
Annette Robichaud, Ph.D., SCIREQ Scientific Respiratory Equipment Inc.. Lennart K.A. Lundblad, Ph.D., Meakins-Christie Labs, McGill University
Wayne Mitzner, Ph.D., John Hopkins Bloomberg School of Public Health
Christian Mühlfeld, Ph.D., Hannover Medical School
2:30pm Rotate Concurrent Labs
4:00pm Slide Review (Bring your questions and fixed slides for analysis. Note: Do NOT bring fresh tissue to The Jackson Laboratory!) Cory Brayton, D.V.M., ACVP, John Hopkins University School of Medicine Douglas Taatjes, Ph.D., The University of Vermont
6:00pm Dinner
|
12:00 PM |
Thursday, September 26th 8:00am Practical Exercise in Lung Stereology I: Volume Estimation
9:15am Practical Exercise in Lung Stereology II: Surface Area Estimation Christian Mühlfeld, Ph.D., Hannover Medical School
11:00am Considerations for Image Presentation and Imaging Ethics Douglas Taatjes, Ph.D., The University of Vermont
12:00pm Humanized Mice and Patient Derived Xenografts (PDX) Brian Soper, Ph.D., The Jackson Laboratory
1:00pm Lunch
6:00pm Dinner |
Provided lodging will be at the gorgeous Highseas Conference Center, a Georgian style mansion built in 1912, overlooking the entrance to Frenchman’s Bay and adjacent to Acadia National Park. This casual, dormitory-style housing consists of shared bedrooms and bathrooms. Breakfast, lunch, dinner, and snacks will be provided at Highseas, including a lobster and steak dinner one night during the event. Wireless internet access is provided throughout the building and laundry facilities are available on site.
Highseas cannot accommodate families or pets. Please refer to the Bar Harbor Chamber of Commerce for lodging alternatives available.
Highseas check-in: Sunday, September 22nd at 2:00 pm or after
Highseas check-out: You must vacate your room on Friday, September 27th by 9:00 am. You may stay in the common areas on the first floor until 2:00 pm.
Bar Harbor is served by two airports:
New visa rules by the U.S. State Department now in effect beginning in August 2008, visitors traveling to the U.S. from visa waiver countries (i.e. Europe, Japan, Australia, etc.) will have to register online 3 days in advance of travel.
Car
Bar Harbor is approximately 5 hours by car from the greater Boston area. Rental cars are available from Hertz at either nearby airport. Additionally, there is a free Island Explorer bus system which can be used around the island and the national park.
Rideshare options like Uber or Lyft are not commonly available.
Additional Area Information:
Associate Professor of Molecular and Comparative Pathology & Director, Phenotyping Core
Vice President
Animal Use Trainer
Research Specialist
Director of Clinical Science
Technical Information Scientist
Professor
Associate Professor
Associate Director, Courses & Conferences | She/Her
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