Techniques in Modeling Human Cancer in Mice

8:00AM   
Course Evaluations
Sharon LaBrie, M.S., University of Maine Center for Research and Evaluation

8:15AM   
Laboratory Mouse – History, genetics, tools, nomenclature and more
David Threadgill, Ph.D., Texas A&M University

9:15AM   
Van transports participants to GRB 3rd Floor Training Laboratory

9:30AM   
Mouse Biomethodology: Handling, restraint, mandibular blood collection, IP and tail vein injection
Jennifer Corrigan, M.S., The Jackson Laboratory
Kristin Cough, B.S., The Jackson Laboratory

12:00PM   
Lunch

12:45PM   
Necropsy
Stephanie Montgomery, Ph.D., DVM, University of North Carolina   

2:45PM    
Break

3:00PM   
Pathology review (bring your own fixed slides for analysis)
Stephanie Montgomery, Ph.D., DVM, University of North Carolina

4:00PM  
Tumor heterogeneity
Chelsea Sievers, B.S., University of Wisconsin

5:00PM    
Van transports participants back to Highseas Conference Center

6:00pm   
Dinner

7:00PM   
Pathology Exercise
Stephanie Montgomery, Ph.D., DVM, University of North Carolina
Tips on Good Specimen Sampling & Fixation
Stephanie Montgomery, Ph.D., DVM, University of North Carolina

Techniques in Modeling Human Cancer in Mice

8:30AM   
Models of Breast Cancer
Amy Moser, Ph.D., University of Wisconsin

9:30AM   
Mammary metastasis models and use of cancer cell lines
Doug Hurst, Ph.D., University of Alabama at Birmingham

10:30AM   
Van transports participants to GRB 3rd Floor Training Laboratory

10:45AM   
Dissection of mammary glands, whole mount preparation, whole mount analysis
Amy Moser, Ph.D., University of Wisconsin
Doug Hurst, Ph.D., University of Alabama at Birmingham

12:45PM   
Lunch

1:30PM   
Mammary gland palpations, tumor and fat pad collection, lung collection, lymph node collection
Amy Moser, Ph.D., University of Wisconsin
Doug Hurst, Ph.D., University of Alabama at Birmingham

3:30PM   
Break

3:45PM    
Fat pad clearing for mammary cell transplantation, orthotopic injections into the mammary fat pad
Amy Moser, Ph.D., University of Wisconsin
Doug Hurst, Ph.D., University of Alabama at Birmingham

5:15PM    
Van transports participants back to Highseas Conference Center

6:00PM   
Dinner

7:00pm   
Models of colon cancer
David Threadgill, Ph.D., Texas A&M University

Techniques in Modeling Human Cancer in Mice

8:30AM   
Predicting colon polyp fate and response to therapy
Richard Halberg, Ph.D., University of Wisconsin

9:30AM   
Van transports participants to GRB 3rd Floor Training Laboratory

9:45AM   
Rotating Sessions
Group 1: Endoscopy techniques
Group 2: Dissection and tumor quantification    
David Threadgill, Ph.D. & Carolina Mantilla Rojas, B.S., Texas, A&M University
Richard Halberg, Ph.D., Chelsea Sievers, B.S., & Quincy Rosemarie, B.S., University of Wisconsin, 
Alyssa Leystra, Ph.D., Fox Chase Cancer Center

10:45AM   
Switch Groups
Group 2: Endoscopy techniques
Group 1: Dissection and tumor quantification    
David Threadgill, Ph.D. & Carolina Mantilla Rojas, B.S., Texas, A&M University
Richard Halberg, Ph.D., Chelsea Sievers, B.S., & Quincy Rosemarie, B.S., University of Wisconsin,
Alyssa Leystra, Ph.D., Fox Chase Cancer Center

11:45AM  
Lunch

12:45PM  
Organoid cultures and their utility
Alyssa Leystra, Ph.D., Fox Chase Cancer Center

1:15PM     
Preparation of organoid cultures
Alyssa Leystra, Ph.D., Fox Chase Cancer Center
Chelsea Sievers, B.S., University of Wisconsin
Quincy Rosemarie, B.S., University of Wisconsin

Techniques in Modeling Human Cancer in Mice

8:15AM  
PDX
Lenny Shultz, Ph.D., The Jackson Laboratory

9:15AM  
Break

9:30AM    Xenografting (Subcutaneous PDX and kidney capsule)
Bonnie Lyons, D.V.M., Jennifer Corrigan, M.S., & Andree Lapierre, B.S., The Jackson Laboratory

11:30AM   
Break

11:45AM   
PDX data collection and analysis
Joel Graber, Ph.D., The Jackson Laboratory

12:45PM   
Lunch

1:30PM
Blood Cancer (GRB)
Jennifer Trowbridge, Ph.D., The Jackson Laboratory

2:30PM  
Leukemia/Lymphoma Techniques
Muneer Hasham, Ph.D., & Jane Branca, The Jackson Laboratory

4:30PM   
Introducing adenovirus into the colon
TBA, Texas, A&M University

5:30PM   
Van transports participants back to Highseas Conference Center

6:00PM    
Lobster Dinner

7:00PM   
Participant presentations

Techniques in Modeling Human Cancer in Mice

8:30AM   
Models of Lung Cancer
Katerina Politi, Ph.D., Yale University

9:30AM  
Translational Value of Mouse Models of Lung Cancer
Margie Clapper, Ph.D., Fox Chase Cancer Center

10:30AM   
Van transports participants to GRB 3rd Floor Training Laboratory

10:45AM   
Lung Preparation and Injections
Margie Clapper, Ph.D., Fox Chase Cancer Center
Katerina Politi, Ph.D., Yale University

12:30PM 
Lunch

1:30PM   
Models of prostate and ovarian cancer
Nigel Crawford, M.B., Ch.B., Ph.D., NHGRI

2:30PM   
Break

2:45pm   
Palpating for internal tumors, prostate preparation and injections
Nigel Crawford, M.B., Ch.B., Ph.D., NHGRI

5:30PM    
Van transports participants back to Highseas Conference Center

6:00PM   
Dinner

7:00PM   
Participant presentations

Techniques in Modeling Human Cancer in Mice

11:30AM   
Van transports participants to JAX Main Lobby

11:45AM   
Rotating Sessions
Group 1 =  Mouse Ultrasound Demo, CT and Xenogen Imaging (FGB 44) **
Jennifer Ryan, B.S., The Jackson Laboratory
Fred Roberts, B.S., VisualSonics, Inc.
Group 2 = Lunch

1:00PM   
Switch Groups
Group 2 =  Mouse Ultrasound Demo, CT and Xenogen Imaging (FGB 44) **
Jennifer Ryan, B.S., The Jackson Laboratory
Fred Roberts, B.S., VisualSonics, Inc.
Group 1 = Lunch

2:15PM    
Break

2:30PM    
Brain cancer models
Raquel Sitcheran, Ph.D., Texas A & M Health Science Center

3:30PM    Brain isolation, intracranial injection and perfusion
Raquel Sitcheran, Ph.D., Texas A & M Health Science Center

5:30PM    
Van transports participants back to Highseas Conference Center

6:00PM   
Dinner

7:00PM   
Perspectives gained and needed