Workshop on Techniques in Modeling Human Cancer in Mice

Techniques in Modeling Human Cancer in Mice

8:00AM   
Course Evaluations
Sharon LaBrie, M.S., University of Maine Center for Research and Evaluation

8:15AM   
Laboratory Mouse – History, genetics, tools, nomenclature and more
David Threadgill, Ph.D., Texas A&M University

9:15AM   
Van transports participants to GRB 3rd Floor Training Laboratory

9:30AM   
Mouse Biomethodology: Handling, restraint, mandibular blood collection, IP and tail vein injection
Jennifer Corrigan, M.S., The Jackson Laboratory
Kristin Cough, B.S., The Jackson Laboratory

12:00PM   
Lunch

12:45PM   
Necropsy
Stephanie Montgomery, Ph.D., DVM, University of North Carolina   

2:45PM    
Break

3:00PM   
Pathology review (bring your own fixed slides for analysis)
Stephanie Montgomery, Ph.D., DVM, University of North Carolina

4:00PM  
Managing long-term cancer studies
Selene Howe, B.S., Texas A&M University

4:30PM
Role of environment in cancer modeling
Danila Cuomo, M.S., Texas A&M University

5:00PM    
Van transports participants back to Highseas Conference Center

6:00PM  
Dinner

7:00PM   
Pathology Exercise
Stephanie Montgomery, Ph.D., DVM, University of North Carolina

7:30PM
Tips on Good Specimen Sampling & Fixation
Stephanie Montgomery, Ph.D., DVM, University of North Carolina

Techniques in Modeling Human Cancer in Mice

8:30AM   
Models of Breast Cancer
Amy Moser, Ph.D., University of Wisconsin

9:30AM   
Mammary metastasis models and use of cancer cell lines
Doug Hurst, Ph.D., University of Alabama at Birmingham

10:30AM   
Van transports participants to GRB 3rd Floor Training Laboratory

10:45AM   
Dissection of mammary glands, whole mount preparation, whole mount analysis
Amy Moser, Ph.D., University of Wisconsin
Doug Hurst, Ph.D., University of Alabama at Birmingham

12:45PM   
Lunch

1:30PM   
Mammary gland palpations, tumor and fat pad collection, lung collection, lymph node collection
Amy Moser, Ph.D., University of Wisconsin
Doug Hurst, Ph.D., University of Alabama at Birmingham

3:30PM   
Break

3:45PM    
Fat pad clearing for mammary cell transplantation, orthotopic injections into the mammary fat pad
Amy Moser, Ph.D., University of Wisconsin
Doug Hurst, Ph.D., University of Alabama at Birmingham

5:15PM    
Van transports participants back to Highseas Conference Center

6:00PM   
Dinner

7:00pm   
Models of colon cancer
David Threadgill, Ph.D., Texas A&M University

Techniques in Modeling Human Cancer in Mice

8:30AM   
Predicting colon polyp fate and response to therapy
Richard Halberg, Ph.D., University of Wisconsin

9:30AM   
Van transports participants to GRB 3rd Floor Training Laboratory

9:45AM   
Rotating Sessions
Group 1: Endoscopy techniques
Group 2: Dissection and tumor quantification    
David Threadgill, Ph.D. & Danila Cuomo, B.S., Selene Howe, B.S., Texas, A&M University
Richard Halberg, Ph.D., University of Wisconsin
Alyssa Leystra, Ph.D., Fox Chase Cancer Center

10:45AM   
Switch Groups
Group 2: Endoscopy techniques
Group 1: Dissection and tumor quantification    
David Threadgill, Ph.D. & Danila Cuomo, B.S., Selene Howe, B.S., Texas, A&M University
Richard Halberg, Ph.D., University of Wisconsin
Alyssa Leystra, Ph.D., Fox Chase Cancer Center

11:45AM  
Lunch

12:45PM  
Organoid cultures and their utility
Alyssa Leystra, Ph.D., Fox Chase Cancer Center

1:15PM     
Preparation of organoid cultures
Alyssa Leystra, Ph.D., Fox Chase Cancer Center
David Threadgill, Ph.D. & Danila Cuomo, B.S., Selene Howe, B.S., Texas, A&M University
Richard Halberg, Ph.D., University of Wisconsin

7:00PM
Imaging technologies as they relate to cancer research
Mohammed Farhoud, M.S., Emit Imaging

Techniques in Modeling Human Cancer in Mice

8:30am
Models of Lung Cancer
Katerina Politi, Ph.D., Yale University

9:30am
Translational Value of Mouse Models of Lung Cancer
Margie Clapper, Ph.D., Fox Chase Cancer Center

10:30am
Van transports participants to GRB 3rd Floor Training Laboratory

10:45am
Lung Preparation and Injections
Margie Clapper, Ph.D., Fox Chase Cancer Center
Katerina Politi, Ph.D., Yale University

12:30pm
Lunch

1:30pm
Leukemia/Lymphoma techniques: lymph node dissection, spleen dissection and splenocyte isolation, bone marrow isolation
Muneer Hasham, Ph.D., and Jane Branca, The Jackson Laboratory

4:00pm
Introducing adenovirus into the colon
TBA Texas, A&M University

5:00pm
Van transports participants back to Highseas Conference Center

6:00pm
Dinner

Techniques in Modeling Human Cancer in Mice

8:00am
Van transports participants to GRB 3225 Conference Room

8:15am
Humanized Mice and PDX
Brian Soper, Ph.D., The Jackson Laboratory

9:15am
Break

9:30am
Xenografting (Subcutaneous PDX and kidney capsule)
Bonnie Lyons, D.V.M., Jennifer Corrigan, M.S. and Andree Lapierre, B.S., The Jackson Laboratory

11:30am
Break

11:45am
PDX data collection and analysis
Joel Graber, Ph.D., The Jackson Laboratory

12:45pm
Lunch

1:30pm
Models of prostate and ovarian cancer
Magdalena Grabowska, Ph.D., Case Western Reserve University

2:30pm
Break

2:45pm
Prostate dissection, prostate injection and surgical orchiectomy
Magdalena Grabowska, Ph.D., Case Western Reserve University
Jennifer Corrigan, M.S., The Jackson Laboratory

5:30pm
Van transports participants back to Highseas Conference Center

6:00pm
Dinner

7:00pm
Participant presentations

Techniques in Modeling Human Cancer in Mice

10:00AM
Participant Presentations(if not completed Friday evening)

11:30AM   
Van transports participants to JAX Main Lobby

11:45AM   
Rotating Sessions
Group 1 =  Mouse Ultrasound Demo, CT and Xenogen Imaging (FGB 44) **
Jennifer Ryan, B.S., The Jackson Laboratory
Fred Roberts, B.S., VisualSonics, Inc.
Selene Howe, B.S., Texas A&M University
Group 2 = Lunch

1:00PM   
Switch Groups
Group 2 =  Mouse Ultrasound Demo, CT and Xenogen Imaging (FGB 44) **
Jennifer Ryan, B.S., The Jackson Laboratory
Fred Roberts, B.S., VisualSonics, Inc.
Selene Howe, B.S., Texas A&M University
Group 1 = Lunch

2:15PM    
Break

2:30PM    
Brain cancer models
Raquel Sitcheran, Ph.D., Texas A & M Health Science Center

3:30PM    Brain isolation, intracranial injection and perfusion
Raquel Sitcheran, Ph.D., Texas A & M Health Science Center
Jennifer Corrigan, M.S., The Jackson Laboratory

5:30PM    
Van transports participants back to Highseas Conference Center

6:00PM   
Dinner

7:00PM   
Perspectives gained and needed