Therapeutic Antibody
Evaluation Services

The JAX FcRn portfolio includes flexible, in vivo models that deliver accurate data to get your therapeutic candidate into the clinic faster.

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From preclinical to clinical

The FcRn models are humanized mice models that are deficient in murine FcRn and over-express human FcRn. Developed by JAX professor Derry Roppenian, Ph.D., these mice allow for

  • faster, predicative clinically-relevant data,
  • an effective alternative to non-human primates,
  • preclinical pharmacokinetic (PK) analysis of therapeutic candidates,
  • utilizing much lower quantities of a given therapeutic candidate,
  • and wide-ranging applications, including immuno-oncology, autoimmune research, among others

Get relevant translational and fast half-life comparison data

Developed by JAX Professor Derry Roopenian, these humanized mouse models are deficient in murine FcRn and over-express human FcRn. Utilize our in-house expertise and get relevant translational and quick half-life comparison data by having our In Vivo Pharmacology Services test your therapeutic antibodies and albumin-conjugates.

Clinical Relevancy of Preclinical Models for Pharmacokinetics 

Model Cost Correlation with Human Data
In Vitro Low Low
Standard mice Moderate Low
Humanized FcRn mice Moderate Very good
Non-human primates High Very good

Featured FcRn Models

B6.Cg-Fcgrttm1Dcr Tg(FCGRT)32Dcr/DcrJ (014565) mice have a null mutation for the mouse gene and a transgene expressing the human FcRn under the endogenous promoter (hTg32). These mice have the highest, most human-like protection of humanized IgG and are the best model for use when maximum half-life data is required.

B6.Cg-Fcgrttm1Dcr Tg(CAG-FCGRT)276Dcr/DcrJ (004919) mice carry a null mutation for the mouse gene and a transgene expressing human FcRn. Mice hemizygous for the human FcRn transgene (hTg276) are best suited to detect subtle differences in antibody persistence in vivo.

B6.Cg-Fcgrttm1Dcr Prkdcscid Tg(FCGRT)32Dcr/DcrJ (018441) mice express the hTg32 transgene and are immunodeficient. These mice have the highest, most human-like protection of humanized IgG and are useful in evaluating Fc-domain based therapeutics that are potentially immunogenic or involve xenografts.

B6.Cg-Alb<em12Mvw> Fcgrt<tm1Dcr> Tg(FCGRT)32Dcr/MvwJ (025201) albumin knockout hTg32 mice are an effective, human-like model for characterization of the pharmacokinetics of albumin-conjugates, or therapeutics carried by albumin.

FcRn Humanized Mouse Models

Model Endogenous promoter Longest half-life Can see subtle differences
in PK values
Immuno-deficient Albumin PK

FcRn Tg32 (014565)

Yes Yes No No No

FcRn Tg276 (004919)

No No Yes No No

Tg32 Scid (018441)

Yes Yes No Yes No

Tg276 Scid* (021146)

No No Yes Yes No

Tg276 Rag1* (016919)

No No Yes Yes No

Tg32 Alb KO (025201

Yes Yes No  No Yes

*Only available via cryorecovery

Example Studies

Example Pharmacokinetic study design

Note: All studies are designed to client specifications

  • A standard study uses 6 B6.Cg-Fcgrttm1Dcr Tg(FCGRT)32Dcr/DcrJ (014565) mice.
  • Test articles and a vehicle control are administered via IV.
  • Blood samples are collected 1, 2, 6, 10, 14, 18, 22, and 26 days after test article administration.
  • The concentration of the test article is quantified by ELISA and PK Solutions software is used to calculate the pharmacokinetic data.

 Example Pharmacodynamic study design

 Note: All studies are designed to client specifications

  • A standard study uses 6 B6.Cg-Fcgrttm1Dcr Tg(FCGRT)32Dcr/DcrJ (014565) mice.
  • Human IgG is administered via IV injection and blood collected 24 hours later.
  • Test articles and a vehicle control are administered via IV 1 hour after the blood collection.
  • Blood samples are collected at 32, 48, 56, 72, 96, 120, and 144 hours.
  • The concentration of the human IgG or albumin can be quantified by ELISA and PK Solutions software is used to calculate the pharmacodynamic data.