JAX 遗传质量控制流程

JAX 严格的遗传质量控制流程(GQC)提早在小鼠育种之前, 检测发现繁殖错误并立即消除,从而有效地防止遗传污染。

Core components

1. Highly skilled animal caretakers

  • Ongoing professional development includes comprehensive genetics and animal husbandry courses offered by JAX and other scientists.
  • Experienced veterans pass on proficiency in recognizing strain characteristics and deviant mice.

2. Rigorous colony management protocols

  • Adherence to proven mouse husbandry practices
  • Physically isolated foundation, expansion and production stocks
  • Detailed foundation and expansion stock pedigree records
  • Foundation and expansion stock generations limited to less than 10 from the main pedigree line
  • Production stocks systematically refreshed with foundation stock mice

3. Systematic screens for variant genotypes and phenotypes

Verifying genetic background
  • All foundation stock breeders are genotyped periodically with a unique JAX SNP marker panel to look for genetic contamination (Petkov et al. 2004;Petkov et al. 2004). (Any genetically contaminated mice are not bred.)
  • At least once annually, expansion and production colony mice from each strain are randomly selected and SNP-genotyped. (Any mice suspected of being genetically contaminated are removed and examined.)
Verifying mutant alleles in mutant stocks
  • Allele-specific assays are used to verify mutant alleles of genetically engineered and spontaneous mutants.
Discovering phenotypic variants
  • Our highly skilled animal care technicians are constantly on the lookout for any phenotype deviations—including coat color, behavioral, and physical abnormalities.
  • JAX scientists examine and determine causes and heritability of new and interesting deviant phenotypes.
  • Deviant mice are removed from breeding colonies, and parent strains are closely monitored for phenotype recurrence.

Contact Us

Call: 1-800-422-6423 (US, Canada & Puerto Rico)
Call: 1-207-288-5845 (from any location)
Email: jaxservices@jax.org