1. Highly skilled animal caretakers
- Ongoing professional development includes comprehensive genetics and animal husbandry courses offered by JAX and other scientists.
- Experienced veterans pass on proficiency in recognizing strain characteristics and deviant mice.
2. Rigorous colony management protocols
- Adherence to proven mouse husbandry practices
- Physically isolated foundation, expansion and production stocks
- Detailed foundation and expansion stock pedigree records
- Foundation and expansion stock generations limited to less than 10 from the main pedigree line
- Production stocks systematically refreshed with foundation stock mice
3. Systematic screens for variant genotypes and phenotypes
Verifying genetic background
- All foundation stock breeders are genotyped periodically with a unique JAX SNP marker panel to look for genetic contamination (Petkov et al. 2004;Petkov et al. 2004). (Any genetically contaminated mice are not bred.)
- At least once annually, expansion and production colony mice from each strain are randomly selected and SNP-genotyped. (Any mice suspected of being genetically contaminated are removed and examined.)
Verifying mutant alleles in mutant stocks
- Allele-specific assays are used to verify mutant alleles of genetically engineered and spontaneous mutants.
Discovering phenotypic variants
- Our highly skilled animal care technicians are constantly on the lookout for any phenotype deviations—including coat color, behavioral, and physical abnormalities.
- JAX scientists examine and determine causes and heritability of new and interesting deviant phenotypes.
- Deviant mice are removed from breeding colonies, and parent strains are closely monitored for phenotype recurrence.
Call: 1-207-288-5845 (from any location)