Wayne Mitzner, Ph.D.
ProfessorJohns Hopkins Bloomberg School of Public Health
My interests in basic mechanisms underlying lung structure and function involve experimental work in several species over more than 30 years, with continuous funding from individual and program grants from NIH. In the late 1980s my lab was one of the first to routinely quantify lung function in mouse lungs, and we published the original study documenting the genetic variation in function in various mouse strains in vivo. This early work over 25 years ago has been followed up in many studies concerned with using a host of pulmonary function and quantitative morphologic measurements to probe mechanisms underlying various lung diseases in mouse models. My lab is involved with many other investigators as collaborator and/or consultant for their pulmonary function tests. I also oversee a Mouse Pulmonary Function facility at Johns Hopkins that is funded by several multidisciplinary grants from the National Institute of Health, and I am now primary director at the annual course on mouse pulmonary function and quantitative lung morphology at the Jackson Laboratories. In addition I was one of the participants of the joint ATS and ERS Task force document to standardize proper stereologic principles as applied to mouse lung histology. I have been involved with quantitative lung histology and pathology for many years, and I was a lecturer in a 2016 international course at the Welcome Trust on “Improving reproducibility of pathology endpoints in mouse challenge models.” My lab has also published several papers and videos showing how to intubate mice so that pulmonary function and even BAL can be assessed in individual mice in longitudinal studies. Overall, my lab has the ability to tightly link modern lung mechanics, pulmonary function, and quantitative morphology with state-of-the-art imaging. In addition to doing conventional pulmonary function measurements in mice, we now have the ability to measure the lung’s diffusing capacity in mice and have shown this to be the most sensitive metric to detect pathologic changes in nearly all mouse models of lung disease. This DLCO is also the only pulmonary function measurement that is common and easy to do in both humans and mice. My currently funded research program deals with the role of macrophage phenotypes in the progression of chronic emphysema.