Protocol 30103: Separated PCR Assay - GAL Panel-B6J vs NOD

NOD.Cg-Tg(K18-ACE2)2Prlmn Prkdc^scid Il2rg^tm1Wjl/J

Stock No: 034901

Version 1.0

Notes

The genotyping protocol(s) presented here have been optimized for reagents and conditions used by The Jackson Laboratory (JAX). To genotype animals, JAX recommends researchers validate the assay independently upon receipt of animals into their facility. Reaction cycling temperature and times may require additional optimization based on the specific genotyping reagents used.

Expected Results

JAX Protocol

Protocol Primers

Primer	5' Label	Sequence 5' → 3'	3' Label	Primer Type	Reaction	Note

Strains Using This Protocol

Stock Number	Strain Name
035002	NOD.Cg-Prkdc^scid Il2rg^tm1Wjl Ace2^tm1(ACE2)Dwnt/J
034901	NOD.Cg-Tg(K18-ACE2)2Prlmn Prkdc^scid Il2rg^tm1Wjl/J
2 strains use this protocol