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Tg=120bp
IPC=74bp
Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
31698 | ACG CTC GTC GTT TGG TAT G | Mutant Forward | A | |||
31699 | CTG ACA ACG ATC GGA GGA C | Mutant Reverse | A | |||
31700 | Fluorophore-1 | TCA AGG CGA GTT ACA TGA TCC | Quencher-1 | MUT Probe | ||
oIMR1544 | CAC GTG GGC TCC AGC ATT | Internal Positive Control Forward | A | |||
oIMR3580 | TCA CCA GTC ATT TCT GCC TTT G | Internal Positive Control Reverse | A | |||
TmoIMR0105 | Fluorophore-2 | CCA ATG GTC GGG CAC TGC TCA A | Quencher-2 | IC Probe |
Component | Final Concentration |
---|---|
Kapa Probe Fast QPCR | 1.00 X |
ddH2O | |
31698 | 0.40 uM |
31699 | 0.40 uM |
oIMR1544 | 0.40 uM |
oIMR3580 | 0.40 uM |
Wt Probe | 0.15 uM |
Mutant Probe | 0.15 uM |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 95.0 | -- | |
2 | 95.0 | -- | |
3 | 60.0 | -- | |
4 | -- | repeat steps 2-3 for 40 cycles | |
5 | 40.0 | -- | Forever |
Stock Number | Strain Name |
---|---|
037318 | B6(Cg)-H3c6em1Sjes/J |
037333 | B6.Cg-Chodlem1(cre)Ngai/TasicJ |
029481 | B6.Cg-Pax9em1(cre/ERT2)Ychai/J |
034424 | B6;129S6-Sncgem1(flpo)Tasic/J |
033120 | C57BL/6J-Il2rgem1Lutzy/J |
033122 | C57BL/6J-Il2rgem3Lutzy/J |
033032 | C57BL/6J-Rab39bem6Lutzy/Mmjax |
034905 | C57BL/6J-Trex1em1Lutzy/J |
8 strains use this protocol |