For in-depth product & services help, ask our
Technical Information Scientists
Mutant = AGACAGTCCTTC
Wild type = GGGGAATTCATC
>chr5:137835515-137835681 167bp GGGTCAACCAACCTGAAAGC TATGAAAGGCAGGGAGGAGT
The WT Probe (primer 54453) anneals over the nucleotide sequence containing mouse genomic variation rs215118515.
SEQ
Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
54451 | GGG TCA ACC AAC CTG AAA GC | Forward | A | |||
54452 | TAT GAA AGG CAG GGA GGA GT | Reverse | A | |||
54453 | Fluorophore-1 | ATT TCC ATG GGG AAT TCA TCT AC | Quencher-1 | WT Probe | ||
54454 | Fluorophore-2 | AAG GAT TTC CAT AGA CAG TCC TTC TAC | Quencher-2 | MUT Probe |
Component | Final Concentration |
---|---|
Kapa Probe Fast QPCR | 1.00 X |
ddH2O | |
54451 | 0.40 uM |
54452 | 0.40 uM |
Wt Probe | 0.15 uM |
Mutant Probe | 0.15 uM |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 95.0 | -- | |
2 | 95.0 | -- | |
3 | 60.0 | -- | |
4 | -- | repeat steps 2-3 for 40 cycles | |
5 | 40.0 | -- | Forever |