For in-depth product & services help, ask our
Technical Information Scientists
Mutant (embryonic lethal) = 100 bp
Heterozygote = 100 bp and 250 bp
Wild type = 250 bp
Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
22907 | TGT CTT TCC CCA GCA CAG T | Wild type Forward | A | |||
22908 | CTG CAT AGT ACG CTA TAC CCT GT | Common | A | |||
oIMR9592 | GCA GGT CGA GGG ACC TAA TA | Mutant Forward | A |
Component | Final Concentration |
---|---|
ddH2O | |
Kapa 2G HS buffer | 1.30 X |
MgCl2 | 2.60 mM |
dNTP KAPA | 0.26 mM |
22907 | 0.50 uM |
22908 | 0.50 uM |
oIMR9592 | 0.50 uM |
Glycerol | 6.50 % |
Dye | 1.00 X |
Kapa 2G HS taq polym | 0.03 U/ul |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 94.0 | -- | |
2 | 94.0 | -- | |
3 | 65.0 | -- | -0.5 C per cycle decrease |
4 | 68.0 | -- | |
5 | -- | repeat steps 2-4 for 10 cycles (Touchdown) | |
6 | 94.0 | -- | |
7 | 60.0 | -- | |
8 | 72.0 | -- | |
9 | -- | repeat steps 6-8 for 28 cycles | |
10 | 72.0 | -- | |
11 | 10.0 | -- | hold |
Stock Number | Strain Name |
---|---|
008180 | 129S/Sv-Krastm4Tyj/J |
033964 | B6.129(Cg)-Ptf1atm2(cre/ESR1)Cvw Krastm4Tyj Ptentm1Hwu/J |
032435 | B6.129-Krastm4Tyj Trp53tm1Brn/J |
008179 | B6.129S4-Krastm4Tyj/J |
035169 | B6.Cg-Krastm4Tyj Apctm1Tno Tg(CDX2-cre/ERT2)752Erf/MaraJ |
019104 | B6N.Cg-Krastm4Tyj/CjDswJ |
032429 | STOCK Krastm4Tyj Trp53tm1Brn Tg(Pdx1-cre/Esr1*)#Dam/J |
7 strains use this protocol |