For in-depth product & services help, ask our
Technical Information Scientists
Mutant = A, G, C, C
Wild type = G, A, T, T
>chr14:27966492+27966587 96bp GCTGGAGAGATCCGTGATATG AAAGGGGTTGTATCAGCTACTCAC
AGAGAAGGGGACCCTAGCTGGAGAGATCCGTGATATGAAAGATATGTTAGAAGTAAA(g/a)GAAAGGAAAATCA(a/g)TGT(t/c)CT(t/c)CAGAAAAAAGTGAGTAGCTGATACAACCCCTTTAACCTG
Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
50286 | GCT GGA GAG ATC CGT GAT ATG | Forward | A | |||
50287 | AAA GGG GTT GTA TCA GCT ACT CAC | Reverse | A | |||
50288 | Fluorophore-1 | AGG AAA GGA AAA TCA ATG TTC TTC A | Quencher-1 | WT Probe | ||
50289 | Fluorophore-2 | AAG AAA GGA AAA TCA GTG TCC TCC | Quencher-2 | MUT Probe |
Component | Final Concentration |
---|---|
Kapa Probe Fast QPCR | 1.00 X |
ddH2O | |
50286 | 0.40 uM |
50287 | 0.40 uM |
Wt Probe | 0.15 uM |
Mutant Probe | 0.15 uM |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 95.0 | -- | |
2 | 95.0 | -- | |
3 | 60.0 | -- | |
4 | -- | repeat steps 2-3 for 40 cycles | |
5 | 40.0 | -- | Forever |