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Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
13926 | TGC AAG AAC TCT TCC TCA CG | Forward | B | |||
13927 | CGA TCT CGG CGA ACA CC | Reverse | B | |||
13928 | Fluorophore-1 | ACA TCG GCA AGG TGT GGG TCG | Quencher-1 | |||
oIMR1544 | CAC GTG GGC TCC AGC ATT | Internal Positive Control Forward | A | |||
oIMR3580 | TCA CCA GTC ATT TCT GCC TTT G | Internal Positive Control Reverse | A | |||
TmoIMR0105 | Fluorophore-2 | CCA ATG GTC GGG CAC TGC TCA A | Quencher-2 |
Component | Final Concentration |
---|---|
ddH2O | |
Kapa Probe Fast QPCR | 1.00 X |
oIMR1544 | 0.40 uM |
oIMR3580 | 0.40 uM |
Primer 3 | 0.40 uM |
Primer 4 | 0.40 uM |
Tg Probe | 0.15 uM |
IC Probe | 0.15 uM |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 95.0 | -- | |
2 | 95.0 | -- | |
3 | 60.0 | -- | repeat steps 2-3 for 40 cycles |
Component | Final Concentration |
---|---|
Kapa Probe Fast QPCR | 1.00 X |
ddH2O | |
13926 | 0.40 uM |
13927 | 0.40 uM |
Primer 3 | 0.40 uM |
Primer 4 | 0.40 uM |
Tg Probe | 0.15 uM |
IC Probe | 0.15 uM |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 95.0 | -- | |
2 | 95.0 | -- | |
3 | 60.0 | -- | repeat steps 2-3 for 40 cycles |
Stock Number | Strain Name |
---|---|
024906 | 129-Dp(16Tiam1-Il10rb)8Yey/J |
035506 | B6.Cg-Gt(ROSA)26Sortm3(CEBPB)Myz/J |
038621 | B6;129P2-Ctcftm2.2Gal/J |
003208 | STOCK Dnmt1tm3Jae Hprt1b-m3 Tg(pPWL512hyg)1Ems/J |
031010 | STOCK Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy TgTn(pb-tetO-Pou5f1,-Klf4,-Myc,-Sox2,-mCherry)250Nagy Tg(Pou5f1-EGFP)1Nagy/J |
5 strains use this protocol |