Protocol 21404: Standard PCR Assay - Tg(FXN) repeat copy number

STOCK Fxn^em2.1Lutzy Tg(FXN)YG8Pook/800J

Stock No: 030395

Version 1.0

Notes

The genotyping protocol(s) presented here have been optimized for reagents and conditions used by The Jackson Laboratory (JAX). To genotype animals, JAX recommends researchers validate the assay independently upon receipt of animals into their facility. Reaction cycling temperature and times may require additional optimization based on the specific genotyping reagents used.

Expected Results

Number of Repeats depends on JR and mouse.
Flanking Sequence: 498 bp (subtract from PCR size and divide by 3 for # of repeats present for each animal)

JAX Protocol

Protocol Primers

Primer	5' Label	Sequence 5' → 3'	3' Label	Primer Type	Reaction	Note
43604		GGC TTG AAC TTC CCA CAC GTG TT
43605		AGG ACC ATC ATG GCC ACA CTT

Strains Using This Protocol

Stock Number	Strain Name
008586	B6.Cg-Tg(FXN*)1Sars Fxn^tm1Mkn/J
031007	STOCK Fxn^em2.1Lutzy Tg(FXN)Y47Pook/J
030395	STOCK Fxn^em2.1Lutzy Tg(FXN)YG8Pook/800J
030930	STOCK Fxn^em2.1Lutzy Tg(FXN)YG8Pook/J
024097	STOCK Fxn^tm1Mkn Tg(FXN)Y47Pook/J
024113	STOCK Fxn^tm1Mkn Tg(FXN)YG8Pook/2J
6 strains use this protocol