For in-depth product & services help, ask our
Technical Information Scientists
Taqman qPCR protocols are run on a real time PCR instrument. Use an appropriate instrument specific Fluorophore/Quencher combination.
Mutant= 105 bp
Wild Type = 101 bp
>chr19:24351801-24351901 101bp TCAGTCCCTCAGCACTGTACC CTTCGTCTACTCTGATCCAGCA
Wt Sequence:
Mutant Sequence:
Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
42872 | TCA GTC CCT CAG CAC TGT ACC | Wild type Forward | A | |||
42873 | CTT CGT CTA CTC TGA TCC AGC A | Wild type Reverse | A | |||
42874 | TGC CTT CCA CTT CCT CTC TC | Mutant Forward | A | |||
42875 | CAT TAT ACG AAG TTA TGG GGG ATG | Mutant Reverse | A | |||
42876 | Fluorophore-1 | ATG CTG TAA TTG GCT ACT CTC GAG TAT AGG | Quencher-1 | WT Probe | ||
42877 | Fluorophore-2 | CCC TGG GAA CAA CCT TGA GTG AC | Quencher-2 | MUT Probe |
Component | Final Concentration |
---|---|
Kapa Probe Fast QPCR | 1.00 X |
ddH2O | |
42872 | 0.40 uM |
42873 | 0.40 uM |
42874 | 0.40 uM |
42875 | 0.40 uM |
Wt Probe | 0.15 uM |
Mutant Probe | 0.15 uM |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 95.0 | -- | |
2 | 95.0 | -- | |
3 | 60.0 | -- | |
4 | -- | repeat steps 2-3 for 40 cycles | |
5 | 40.0 | -- | Forever |