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Mutant = A/A
Heterozygote = -/A
Wild type = -/-
Mutation is an A insertion
Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
26910 | Fluorophore-1 | CGC CCA GCC CCG | Quencher-1 | WT Probe | ||
26911 | Fluorophore-2 | CGC CCA AGC CCC G | Quencher-2 | MUT Probe | ||
28586 | CCG GAG CAT TAA CCT TGT TT | Forward | A | |||
28587 | TGG TGG GTG CGC AGG A | Reverse | A |
Component | Final Concentration |
---|---|
Kapa Probe Fast QPCR | 1.00 X |
ddH2O | |
28586 | 0.40 uM |
28587 | 0.40 uM |
Wt Probe | 0.15 uM |
Mutant Probe | 0.15 uM |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 95.0 | -- | |
2 | 95.0 | -- | |
3 | 60.0 | -- | repeat steps 2-3 for 40 cycles |