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Mutant = ----/----
Heterozygote = GTGT/----
Wild type = GTGT/GTGT
Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
25525 | GCT CAT GGA GGA TGG CTT C | Forward | A | |||
25526 | CAT GTC GTG GCA CAG CAG | Reverse | A | |||
25527 | Fluorophore-1 | CCT GGT GTG TCG CCG G | Quencher-1 | Internal Positive Control Forward | A | |
25528 | Fluorophore-2 | CCC TGG TCG CCG GC | Quencher-2 | Internal Positive Control Reverse | A |
Component | Final Concentration |
---|---|
ddH2O | |
Kapa Probe Fast QPCR | 1.00 X |
25525 | 0.40 uM |
25526 | 0.40 uM |
25527 | 0.40 uM |
25528 | 0.40 uM |
Tg Probe | 0.15 uM |
IC Probe | 0.15 uM |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 95.0 | -- | |
2 | 95.0 | -- | |
3 | 60.0 | -- | |
4 | -- | repeat steps 2-3 for 40 cycles | |
5 | 40.0 | -- | Forever |