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Mutant = G/G
Heterozygote = A/G
Wild type = A/A
Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
24890 | Fluorophore-1 | GCC ACT GCA AAT GTC TTC C | Quencher-1 | WT Probe | ||
24891 | Fluorophore-2 | GCC ACT GCA GAT GTC TTC C | Quencher-2 | MUT Probe | ||
25187 | AAC CAT CTG TGG CTC CTG TC | Forward | A | |||
25188 | AGC ACT GGA CCT ACC TTA TGC | Reverse | A |
Component | Final Concentration |
---|---|
Kapa Probe Fast QPCR | 1.00 X |
ddH2O | |
25187 | 0.40 uM |
25188 | 0.40 uM |
Wt Probe | 0.15 uM |
Mutant Probe | 0.15 uM |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 95.0 | -- | |
2 | 95.0 | -- | |
3 | 60.0 | -- | |
4 | -- | repeat steps 2-3 for 40 cycles | |
5 | 40.0 | -- | Forever |