For in-depth product & services help, ask our
Technical Information Scientists
Mutant = A/A
Heterozygote = G/A
Wild type = G/G
Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
24482 | TGT GGA GTG TTG GAG TGA CA | Forward | A | |||
24483 | GGT GCT TAC ATT ACT TCT TTG TTC C | Reverse | A | |||
24484 | Fluorophore-1 | AGC CAC GGG GTC GCT | Quencher-1 | WT Probe | ||
24488 | Fluorophore-2 | CAG CCA CGA GGT CGC TG | Quencher-2 | MUT Probe |
Component | Final Concentration |
---|---|
Kapa Probe Fast QPCR | 1.00 X |
ddH2O | |
24482 | 0.40 uM |
24483 | 0.40 uM |
Wt Probe | 0.15 uM |
Mutant Probe | 0.15 uM |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 95.0 | -- | |
2 | 95.0 | -- | |
3 | 60.0 | -- | |
4 | -- | repeat steps 2-3 for 40 cycles | |
5 | 40.0 | -- | Forever |