Protocol 20998: Separated PCR Assay - GAL Panel-T1DR

NOD.129(Cg)-Gt(ROSA)26Sor^{tm1.1(CAG-cas9*,-EGFP)Fezh}/J

Stock No: 026557

Version 1.0

Notes

The genotyping protocol(s) presented here have been optimized for reagents and conditions used by The Jackson Laboratory (JAX). To genotype animals, JAX recommends researchers validate the assay independently upon receipt of animals into their facility. Reaction cycling temperature and times may require additional optimization based on the specific genotyping reagents used.

Expected Results

JAX Protocol

Protocol Primers

Primer	5' Label	Sequence 5' → 3'	3' Label	Primer Type	Reaction	Note

Strains Using This Protocol

Stock Number	Strain Name
026431	NOD.129(B6)-Gt(ROSA)26Sor^tm1(CAG-cas9*,-EGFP)Fezh/J
026557	NOD.129(Cg)-Gt(ROSA)26Sor^tm1.1(CAG-cas9*,-EGFP)Fezh/J
026996	NOD.B6-Tg(CD68-EGFP)1Drg/J
3 strains use this protocol