For in-depth product & services help, ask our
Technical Information Scientists
Mut = A
WT= G
GAGGTCACAAGGTTTAAGGTCCTCGTCTATCGCTGTCTTCATTAGCTGCTTGAATTTGC
TGTGTTCCGTTCTAGGCACGACTGCCC(g/a)TGAAGTGGATGGCACCAGAGAGCATTTT
CAGCTGCGTGTACACATTTGAAAGTGATGTCTGGTCCTATGGGATTTTCCTCTGGGAG
Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
24704 | AGG TCC TCG TCT ATC GCT GT | Forward | A | |||
24705 | AAA TGC TCT CTG GTG CCA TC | Reverse | A | |||
24706 | Fluorophore-1 | ACT GCC CGT GAA GTG GAT | Quencher-1 | WT Probe | ||
24707 | Fluorophore-2 | ACT GCC CAT GAA GTG GAT G | Quencher-2 | MUT Probe |
Component | Final Concentration |
---|---|
Kapa Probe Fast QPCR | 1.00 X |
ddH2O | |
24704 | 0.40 uM |
24705 | 0.40 uM |
Wt Probe | 0.15 uM |
Mutant Probe | 0.15 uM |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 95.0 | -- | |
2 | 95.0 | -- | |
3 | 60.0 | -- | |
4 | -- | repeat steps 2-3 for 40 cycles | |
5 | 40.0 | -- | Forever |
Stock Number | Strain Name |
---|---|
039112 | B6.Cg-Ptprca Pepcb KitW-41J Gpi1a/IslabMmjax |
000119 | C57BL/6J-KitW-41J/J |
026622 | NOD.Cg-KitW-41J Tyr + Prkdcscid Il2rgtm1Wjl/ThomJ |
026497 | NOD.Cg-KitW-41J Prkdcscid Il2rgtm1Wjl/WaskJ |
026014 | NOD.Cg-Rag1tm1Mom KitW-41J Il2rgtm1Wjl/EavJ |
5 strains use this protocol |