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Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|
Component | Final Concentration |
---|---|
ddH2O | |
Kapa 2G HS buffer | 1.00 |
MgCl2 | 2.00 |
dNTPS-kapa | 0.20 |
Primer 1 | 0.50 |
Primer 2 | 0.50 |
10x Loading Dye | 0.50 |
Kapa 2G HS taq polym | 0.01 |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 94.0 | -- | |
2 | 94.0 | -- | |
3 | 65.0 | -- | -0.5 C per cycle decrease |
4 | 68.0 | -- | |
5 | -- | repeat steps 2-4 for 10 cycles | |
6 | 94.0 | -- | |
7 | 60.0 | -- | |
8 | 72.0 | -- | |
9 | -- | repeat steps 6-8 for 28 cycles | |
10 | 72.0 | -- | |
11 | 10.0 | -- | hold |
Stock Number | Strain Name |
---|---|
003070 | ALR/LtJ |
026277 | B6.129S2(B10)-Il3tm1Tyb/J |
033315 | B6.129S4(B6J)-Pparatm1Gonz/J |
033903 | B6.129S4(C)-C5ar1tm1Cge/BaoluJ |
026293 | B6.Cg-Gt(ROSA)26Sortm1(LRRK2*R1441C)Djmo/J |
021772 | B6.MRL-Atp6v1b1vtx/Kjn |
017893 | B6;CBA-Tg(Thy1-GCaMP3)6Gfng/J |
028239 | B6J.129(Cg)-Igs2tm1.1(CAG-cas9*)Mmw/J |
028861 | B6J.129S6-Chattm2(cre)Lowl/MwarJ |
025114 | B6N.Cg-Tg(Camk2a-P2rx2/YC3.1)21Khakh/J |
025975 | BXD124/RwwJ |
025985 | BXD161/RwwJ |
035247 | C.Cg-Tg(K18-ACE2)2Prlmn/J |
007600 | C57BL/6J-Chr 10.1PWD/Ph/ForeJ |
006226 | C57BL/6J-Chr 4PWD/Ph/ForeJ |
005304 | C57BL/6NJ |
014535 | CAST.129S1(B6)-Kif18agcd2/JcsMmjax |
000656 | CBA/J |
022527 | D2.Cg-Terctm1Rdp/J |
026558 | FVB.129(B6)-Gt(ROSA)26Sortm1.1(CAG-cas9*,-EGFP)Fezh/J |
021771 | MRL/MpJ-Atp6v1b1vtx/Kjn |
21 strains use this protocol |