For in-depth product & services help, ask our
Technical Information Scientists
This assay cannot distinguish hemi from hom
Tg= 85 bp
IPC = 74 bp
This assay distinguishes GCaMP6s from GCaMP6f and GCaMP3
aagacaggtcacgcagtcagagctataggtcggctgagctcactcgagaacgtctatatcaaggccgacaagcagaagaacggcatcaaggcgaacttc[CaC]atccgccacaacatcgaggacggcggcgtgcagctcgcctaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaacc
Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
27656 | TGG TAG TGG TAG GCG AGC TG | Transgene Reverse | A | |||
31033 | ACA AGC AGA AGA ACG GCA TC | Transgene Forward | A | |||
31034 | Fluorophore-1 | CGA ACT TCC ACA TCC GCC | Quencher-1 | Tg Probe | ||
oIMR1544 | CAC GTG GGC TCC AGC ATT | Internal Positive Control Forward | A | |||
oIMR3580 | TCA CCA GTC ATT TCT GCC TTT G | Internal Positive Control Reverse | A | |||
TmoIMR0105 | Fluorophore-2 | CCA ATG GTC GGG CAC TGC TCA A | Quencher-2 | IC Probe |
Component | Final Concentration |
---|---|
Kapa Probe Fast QPCR | 1.00 X |
ddH2O | |
27656 | 0.40 uM |
31033 | 0.40 uM |
oIMR1544 | 0.40 uM |
oIMR3580 | 0.40 uM |
Wt Probe | 0.15 uM |
Mutant Probe | 0.15 uM |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 95.0 | -- | |
2 | 95.0 | -- | |
3 | 60.0 | -- | |
4 | -- | repeat steps 2-3 for 40 cycles | |
5 | 40.0 | -- | Forever |
Stock Number | Strain Name |
---|---|
024115 | B6.Cg-Igs7tm94.1(tetO-GCaMP6s)Hze Tg(Camk2a-tTA)1Mmay/J |
024104 | B6.Cg-Igs7tm94.1(tetO-GCaMP6s)Hze/J |
025776 | C57BL/6J-Tg(Thy1-GCaMP6s)GP4.12Dkim/J |
024275 | C57BL/6J-Tg(Thy1-GCaMP6s)GP4.3Dkim/J |
028278 | C57BL/6J-Tg(Thy1-GCaMP6s)GP4.6Dkim/J |
024112 | STOCK Gt(ROSA)26Sortm5(ACTB-tTA)Luo Igs7tm94.1(tetO-GCaMP6s)Hze/J |
6 strains use this protocol |