For in-depth product & services help, ask our
Technical Information Scientists
WT=T/T
HET=T/A
HOM=A/A
TGCACCACAGGCCCCACCGCCTGCCCAGTCATGC
AGACCTGCAGCCAAACAGCC(t/a)GGAGTTCCTTCAGC
CCTGATCC
Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
20552 | GCA GAG TTT CCA GAG CCT GA | Forward | ||||
20553 | GAC TGC ACC CAG CTC TGA CT | Reverse |
Component | Final Concentration |
---|---|
ddH2O | |
Kapa 2G HS buffer | 0.24 |
MgCl2 | 0.48 |
dNTP KAPA | 0.05 |
Primer 1 | 0.12 |
Primer 2 | 0.12 |
Kapa 2G HS taq polym | 0.00 |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 94.0 | -- | |
2 | 94.0 | -- | |
3 | 65.0 | -- | -0.5 C per cycle decrease |
4 | 68.0 | -- | |
5 | -- | repeat steps 2-4 for 10 cycles | |
6 | 94.0 | -- | |
7 | 60.0 | -- | |
8 | 72.0 | -- | |
9 | -- | repeat steps 6-8 for 28 cycles | |
10 | 72.0 | -- | |
11 | 10.0 | -- | hold |