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Technical Information Scientists
C57BL/6 = no band, human band approximately 480 bp.
Stock# 012963 = 2-3 bands from ~1000bp -1100 bp
Stock# 008398 = 3-4 bands form ~810 bp -1050 bp
Stock# 012910 = 2-3 bands from ~1070 bp - 1300 bp
JR 24097 contains 9 GAA repeats
JR 24113 contains ~200 GAA repeats
Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
oIMR8595 | GGG ATT GGT TGC CAG TGC TTA AAA GTT AG | Forward | A | |||
oIMR8596 | GAT CTA AGG ACC ATC ATG GCC ACA CTT GCC | Reverse | A |
Component | Final Concentration |
---|---|
ddH2O | |
Kapa 2G HS buffer | 1.30 X |
MgCl2 | 2.60 mM |
dNTPS-kapa | 0.26 mM |
oIMR8595 | 0.50 uM |
oIMR8596 | 0.50 uM |
Glycerol | 6.50 % |
Dye | 1.00 X |
Kapa 2G HS taq polym | 0.03 U/ul |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 94.0 | -- | |
2 | 94.0 | -- | |
3 | 65.0 | -- | -0.5 C per cycle decrease |
4 | 68.0 | -- | |
5 | -- | repeat steps 2-4 for 10 cycles (Touchdown) | |
6 | 94.0 | -- | |
7 | 60.0 | -- | |
8 | 72.0 | -- | |
9 | -- | repeat steps 6-8 for 28 cycles | |
10 | 72.0 | -- | |
11 | 10.0 | -- | hold |