For in-depth product & services help, ask our
Technical Information Scientists
Mutant = ~330 bp
Heterozygote = 263 bp and ~330 bp
Wild type = 263 bp
Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
17895 | CCT AAG GGT TGT CCT GTC CA | Common | ||||
17896 | GGT GAG AAG GCG AAG TCT GA | Wild type Reverse | ||||
oIMR7808 | AAC AGC TCC TCG CCC TTG | Mutant Reverse |
Component | Final Concentration |
---|---|
Kapa Probe Fast QPCR | 0.42 |
ddH2O | |
Primer 1 | 0.03 |
Primer 2 | 0.03 |
Primer 3 | 0.02 |
Primer 4 | 0.02 |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 94.0 | -- | |
2 | 94.0 | -- | |
3 | 65.0 | -- | -0.5 C per cycle decrease |
4 | 68.0 | -- | |
5 | -- | repeat steps 2-4 for 10 cycles | |
6 | 94.0 | -- | |
7 | 60.0 | -- | |
8 | 72.0 | -- | |
9 | -- | repeat steps 6-8 for 28 cycles | |
10 | 72.0 | -- | |
11 | 10.0 | -- | hold |