For in-depth product & services help, ask our
Technical Information Scientists
Mutant = A/A
Heterozygote =G/A
Wild type = G/G
ATA TATTT CTCCA TGAAG GCCTC ACGGT GAAAA TTGGA (G/A)ATTT TGGTT TGGCA ACAGT GAAGT CACGC TGGAG TGGTT CTCAG CAGGT TGAAC AGCCC ACTGG CTCTG TGCT C TAG AT GGTAT ATAAA GAGGT ACTTC CGGAG TGCCA CTTTT AACCT CTA AA ACCAA ACCGT TGTCA CTTCA GTGCG ACCTC ACCAA GAGTC GTCCA ACTTG TCGGG TGACC GAGAC ACGA G ATCTA CC
Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
16425 | AAG TGA AGG AGG CCA GAA GC | Forward | A | |||
16426 | AGC TTC CTC ACC ATC CAC AG | Reverse | A | |||
16427 | Fluorophore-1 | GAA AAT TGG AGA TTT TGG TTT G | Quencher-1 | WT Probe | ||
16428 | Fluorophore-2 | GAA AAT TGG AAA TTT TGG TTT G | Quencher-2 | MUT Probe |
Component | Final Concentration |
---|---|
Kapa Probe Fast QPCR | 1.00 X |
ddH2O | |
16425 | 0.40 uM |
16426 | 0.40 uM |
Wt Probe | 0.15 uM |
Mutant Probe | 0.15 uM |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 95.0 | -- | |
2 | 95.0 | -- | |
3 | 60.0 | -- | |
4 | -- | repeat steps 2-3 for 40 cycles | |
5 | 40.0 | -- | Forever |