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Mutant =GcgG
Heterozygote = (A/G)cg(T/G)
Wild type = AcgT
chr1:106712600-106712731 132bp CTTCCAGCCTGAGAGCAAC AGGGTCAGATGGACCACA
Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
56955 | Fluorophore-1 | CTG CCA GGA CGT CTC CTC TC | Quencher-1 | WT Probe | ||
56956 | Fluorophore-2 | CCA GGG CGG CTC CTC T | Quencher-2 | MUT Probe | ||
56957 | CTT CCA GCC TGA GAG CAA C | Forward | A | |||
56958 | AGG GTC AGA TGG ACC ACA | Reverse | A |
Component | Final Concentration |
---|---|
Kapa Probe Fast QPCR | 1.00 X |
ddH2O | |
56957 | 0.40 uM |
56958 | 0.40 uM |
Wt Probe | 0.15 uM |
Mutant Probe | 0.15 uM |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 95.0 | -- | |
2 | 95.0 | -- | |
3 | 60.0 | -- | |
4 | -- | repeat steps 2-3 for 40 cycles | |
5 | 40.0 | -- | Forever |