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This assay will NOT distinguish hemizygous from homozygous transgenic animals.
Transgene = 288 bp
Internal positive control = 324 bp
Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
9703 | AGG ACG GCG AGT TCA TCT AC | Transgene Forward | mCherry | |||
9704 | TGG TGT AGT CCT CGT TGT GG | Transgene Reverse | mCherry | |||
oIMR7338 | CTA GGC CAC AGA ATT GAA AGA TCT | Internal Positive Control Forward | ||||
oIMR7339 | GTA GGT GGA AAT TCT AGC ATC ATC C | Internal Positive Control Reverse |
Stock Number | Strain Name |
---|---|
024046 | C57BL/6-Tg(Csf1r-HBEGF/mCherry)1Mnz/J |
018542 | FVB-Tg(Kdr-mCherry)1Medi/J |
018067 | FVB-Tg(Prism)1849Htz/J |
018071 | FVB-Tg(Prism)1861Htz/J |
014177 | STOCK Tg(Afp-mCherry)1Hadj/J |
025693 | STOCK Tg(CAG-GFP,-mCherry/Pxn)#Mgin/KleyJ |
017470 | STOCK Tg(Tnc-mCherry)2Pmay/J |
011108 | STOCK Tg(Ttr-RFP)1Hadj/J |
8 strains use this protocol |