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Mutant =TCG/TCG
Heterozygote = CCC/TCG
Wild type = CCC/CCC
Converting to 12ul, email genotyping@jax.org with jpeg
Mutant =TCG/TCG
Heterozygote = CCC/TCG
Wild type = CCC/CCC
ATTTAGATCGAGTAAAGAAACCTGAATGGGTTATTCTGATAGG
TGTCTGTACTCATCTTGGTTGTGTA(CCC/TCG)ATTGCAAAC
GCAGGAGATTTTGGTGGCTATTATTGCCCCTGCCATGGGTC
ACACTATGACGCCTCTGG
Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
16344 | Fluorophore-1 | GGT TGT GTA CCC ATT GCA AAC | Quencher-1 | WT Probe | ||
16345 | Fluorophore-2 | GGT TGT GTA TCG ATT GCA AAC | Quencher-2 | MUT Probe | ||
16346 | GGA GAT TGA CCA GGA AGC TG | Forward | A | |||
16347 | CAT GGC AGG GGC AAT AAT AG | Reverse | A |
Component | Final Concentration |
---|---|
Kapa Probe Fast QPCR | 1.00 X |
ddH2O | |
16346 | 0.40 uM |
16347 | 0.40 uM |
Wt Probe | 0.15 uM |
Mutant Probe | 0.15 uM |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 95.0 | -- | |
2 | 95.0 | -- | |
3 | 60.0 | -- | |
4 | -- | repeat steps 2-3 for 40 cycles | |
5 | 40.0 | -- | Forever |