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Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
10280 | GTA CGG GGA TGT GAT GGA TG | Transgene Forward | A | |||
10281 | CGC AAT CTG ATC ATC TGC AA | Transgene Reverse | A | |||
10282 | Fluorophore-1 | CCA AGC CAT TCA GGG CCT TTG C | Quencher-1 | Tg Probe | ||
oIMR1544 | CAC GTG GGC TCC AGC ATT | Internal Positive Control Forward | A | |||
oIMR3580 | TCA CCA GTC ATT TCT GCC TTT G | Internal Positive Control Reverse | A | |||
TmoIMR0105 | Fluorophore-2 | CCA ATG GTC GGG CAC TGC TCA A | Quencher-2 | IC Probe |
Component | Final Concentration |
---|---|
Kapa Probe Fast QPCR | 1.00 X |
ddH2O | |
10280 | 0.40 uM |
10281 | 0.40 uM |
oIMR1544 | 0.40 uM |
oIMR3580 | 0.40 uM |
Tg Probe | 0.15 uM |
IC Probe | 0.15 uM |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 95.0 | -- | |
2 | 95.0 | -- | |
3 | 60.0 | -- | repeat steps 2-3 for 40 cycles |
Stock Number | Strain Name |
---|---|
010700 | B6.Cg-Tg(Prnp-TARDBP*A315T)95Balo/J |
017933 | B6.Cg-Tg(Prnp-TARDBP*Q331K)103Dwc/J |
028413 | B6;C3-Tg(NEFH-tTA)8Vle Tg(tetO-TARDBP)12Vle/J |
016841 | B6;C3-Tg(tetO-TARDBP)12Vle/J |
017930 | B6N.Cg-Tg(Prnp-TARDBP*Q331K)109Dwc/J |
016608 | C57BL/6-Tg(Prnp-TARDBP)3cPtrc/J |
017604 | C57BL/6-Tg(Prnp-TARDBP*M337V)4Ptrc/J |
016144 | STOCK Tg(Prnp-TARDBP)4Jlel/J |
8 strains use this protocol |