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Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
oIMR9392 | GCC CTA ATG TCG GAA CTG AA | Forward | ||||
oIMR9393 | GTC TGA AGG CTC CCA GCA TA | Reverse |
Component | Final Concentration |
---|---|
ddH2O | |
AB PCR BufferII | 1.00 X |
MgCl2 | 2.00 mM |
dNTP | 0.20 mM |
oIMR9392 | 1.00 |
oIMR9393 | 1.00 |
Taq DNA Polymerase | 0.03 U/ul |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 94.0 | -- | |
2 | 94.0 | -- | |
3 | 60.0 | -- | |
4 | 72.0 | -- | repeat steps 2-4 for 35 cycles |
5 | 72.0 | -- | |
6 | 10.0 | -- | hold |
Component | Final Concentration |
---|---|
ddH2O | 0.00 |
NEB Buffer 3 | 1.50 X |
NsiI |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 37.0 | -- |